Limosilactobacillus ingluviei
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1922 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R2H309|A0A0R2H309_9LACO Phosphoribosylformylglycinamidine synthase subunit PurQ OS=Limosilactobacillus ingluviei OX=148604 GN=purQ PE=3 SV=1
MM1 pKa = 7.57 KK2 pKa = 10.67 NMTKK6 pKa = 10.42 AVVVFATITGNNEE19 pKa = 3.57 DD20 pKa = 3.99 VADD23 pKa = 5.73 LITDD27 pKa = 3.64 ALEE30 pKa = 4.2 NLGVDD35 pKa = 3.5 VDD37 pKa = 4.02 EE38 pKa = 6.17 KK39 pKa = 10.61 EE40 pKa = 3.66 ITMADD45 pKa = 3.23 VADD48 pKa = 5.12 FEE50 pKa = 5.24 DD51 pKa = 4.9 ADD53 pKa = 3.72 ICVVCPYY60 pKa = 10.11 TYY62 pKa = 10.79 DD63 pKa = 3.34 EE64 pKa = 4.73 GALPEE69 pKa = 4.65 EE70 pKa = 4.81 GLDD73 pKa = 4.23 FYY75 pKa = 11.78 DD76 pKa = 4.92 DD77 pKa = 4.18 LKK79 pKa = 11.39 EE80 pKa = 4.3 ADD82 pKa = 4.53 LSGKK86 pKa = 9.92 IYY88 pKa = 10.56 GVAGSGDD95 pKa = 3.41 TFYY98 pKa = 11.67 ADD100 pKa = 3.8 DD101 pKa = 3.73 YY102 pKa = 11.26 CRR104 pKa = 11.84 AVDD107 pKa = 3.8 DD108 pKa = 4.96 FGAAFEE114 pKa = 4.74 SAGATKK120 pKa = 10.16 GAEE123 pKa = 4.08 YY124 pKa = 10.97 VHH126 pKa = 7.08 IDD128 pKa = 4.18 LAPEE132 pKa = 4.48 GDD134 pKa = 5.07 DD135 pKa = 5.09 VDD137 pKa = 4.18 TLDD140 pKa = 4.78 AFAASLVEE148 pKa = 4.09 KK149 pKa = 10.68 AVGKK153 pKa = 10.36
Molecular weight: 16.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.768
IPC2_protein 3.592
IPC_protein 3.605
Toseland 3.376
ProMoST 3.783
Dawson 3.605
Bjellqvist 3.757
Wikipedia 3.554
Rodwell 3.427
Grimsley 3.287
Solomon 3.592
Lehninger 3.554
Nozaki 3.719
DTASelect 3.973
Thurlkill 3.439
EMBOSS 3.567
Sillero 3.732
Patrickios 1.825
IPC_peptide 3.592
IPC2_peptide 3.694
IPC2.peptide.svr19 3.686
Protein with the highest isoelectric point:
>tr|A0A0R2H5T5|A0A0R2H5T5_9LACO Glycerol dehydrogenase OS=Limosilactobacillus ingluviei OX=148604 GN=IV41_GL001825 PE=3 SV=1
MM1 pKa = 6.82 QVKK4 pKa = 8.57 VHH6 pKa = 6.19 QRR8 pKa = 11.84 AQVNQPQQAQANRR21 pKa = 11.84 HH22 pKa = 4.57 QRR24 pKa = 11.84 VQAKK28 pKa = 8.77 VRR30 pKa = 11.84 QRR32 pKa = 11.84 VPVNRR37 pKa = 11.84 HH38 pKa = 4.02 QRR40 pKa = 11.84 VQAKK44 pKa = 7.02 VHH46 pKa = 5.2 QRR48 pKa = 11.84 AQVKK52 pKa = 9.32 VRR54 pKa = 11.84 QQVPANPLLLAPANRR69 pKa = 11.84 LQQAQVNRR77 pKa = 11.84 LQQVQVNRR85 pKa = 11.84 PQRR88 pKa = 11.84 AQVNPLQQAQVNQLLLVQANRR109 pKa = 11.84 LQRR112 pKa = 11.84 AQVV115 pKa = 3.05
Molecular weight: 13.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.389
IPC_protein 12.983
Toseland 13.144
ProMoST 13.642
Dawson 13.144
Bjellqvist 13.144
Wikipedia 13.627
Rodwell 12.749
Grimsley 13.188
Solomon 13.642
Lehninger 13.554
Nozaki 13.144
DTASelect 13.144
Thurlkill 13.144
EMBOSS 13.642
Sillero 13.144
Patrickios 12.486
IPC_peptide 13.656
IPC2_peptide 12.632
IPC2.peptide.svr19 9.284
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1922
0
1922
587619
43
2429
305.7
33.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.523 ± 0.09
0.523 ± 0.016
5.299 ± 0.068
5.089 ± 0.06
3.884 ± 0.038
6.89 ± 0.053
2.37 ± 0.027
5.57 ± 0.053
5.276 ± 0.064
10.484 ± 0.088
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.51 ± 0.026
4.002 ± 0.044
4.142 ± 0.036
5.592 ± 0.062
4.468 ± 0.044
4.875 ± 0.09
6.366 ± 0.05
7.402 ± 0.046
1.158 ± 0.024
3.438 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here