Oleibacter marinus
Average proteome isoelectric point is 5.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3470 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1N7N1X4|A0A1N7N1X4_9GAMM Predicted membrane protein OS=Oleibacter marinus OX=484498 GN=SAMN05421686_106113 PE=4 SV=1
MM1 pKa = 6.31 TTSLFSRR8 pKa = 11.84 ASLLAITVSLMACGGGSGGSSNNDD32 pKa = 3.23 QSPDD36 pKa = 3.47 SADD39 pKa = 3.31 NSTDD43 pKa = 3.46 NPSGGDD49 pKa = 3.44 QNTDD53 pKa = 2.99 NTDD56 pKa = 3.34 TQIVLSDD63 pKa = 3.85 TTWSAGNDD71 pKa = 3.6 RR72 pKa = 11.84 ILGMGFNGDD81 pKa = 3.81 SEE83 pKa = 5.0 SPEE86 pKa = 3.84 LTIMKK91 pKa = 8.34 GAPVSGQDD99 pKa = 3.18 GDD101 pKa = 3.68 IFSIRR106 pKa = 11.84 SVTYY110 pKa = 9.23 PSTEE114 pKa = 3.56 LSEE117 pKa = 4.25 IKK119 pKa = 10.75 SIDD122 pKa = 3.47 NQADD126 pKa = 3.49 RR127 pKa = 11.84 RR128 pKa = 11.84 DD129 pKa = 3.93 FQDD132 pKa = 3.05 MDD134 pKa = 3.33 IVSYY138 pKa = 11.13 NDD140 pKa = 2.94 EE141 pKa = 4.67 SYY143 pKa = 11.56 VVACQDD149 pKa = 3.39 GSDD152 pKa = 3.78 TPGYY156 pKa = 8.19 TSAVSLHH163 pKa = 6.44 IYY165 pKa = 7.77 KK166 pKa = 10.26 TSDD169 pKa = 2.82 TSKK172 pKa = 10.31 VAEE175 pKa = 4.28 IDD177 pKa = 3.89 LVDD180 pKa = 4.1 GSVEE184 pKa = 3.7 ARR186 pKa = 11.84 EE187 pKa = 4.28 CSGIAAEE194 pKa = 4.65 FTQGTSDD201 pKa = 3.79 DD202 pKa = 3.96 MGAVVYY208 pKa = 9.79 YY209 pKa = 10.3 AGNAMTTSGSPQYY222 pKa = 10.71 SYY224 pKa = 11.62 SRR226 pKa = 11.84 IMKK229 pKa = 9.89 VEE231 pKa = 3.73 LTIDD235 pKa = 3.09 TTANVDD241 pKa = 3.11 ATDD244 pKa = 3.87 AVVLDD249 pKa = 5.29 DD250 pKa = 3.5 ISTVVRR256 pKa = 11.84 ASQTEE261 pKa = 4.04 DD262 pKa = 3.02 FMSGVTGFGDD272 pKa = 3.41 HH273 pKa = 6.82 VYY275 pKa = 8.94 YY276 pKa = 10.32 TYY278 pKa = 11.5 YY279 pKa = 10.81 DD280 pKa = 3.89 DD281 pKa = 6.13 SEE283 pKa = 4.33 EE284 pKa = 4.45 TNNLMYY290 pKa = 10.67 ANSGSSPATIRR301 pKa = 11.84 NVDD304 pKa = 3.09 WSANDD309 pKa = 3.68 PFVTGVAQQMLVKK322 pKa = 10.65 DD323 pKa = 3.63 MFVIPGQQATDD334 pKa = 3.57 FDD336 pKa = 5.03 SIYY339 pKa = 10.07 MVSDD343 pKa = 3.9 SAASGIVVAGYY354 pKa = 10.14 RR355 pKa = 11.84 RR356 pKa = 11.84 DD357 pKa = 3.69 MNLKK361 pKa = 9.65 QAMTLSSDD369 pKa = 3.57 TSVQNCSDD377 pKa = 4.39 VITGISDD384 pKa = 3.09 QGVGQKK390 pKa = 9.53 LWCHH394 pKa = 6.64 DD395 pKa = 3.56 ATDD398 pKa = 3.6 SGNIIEE404 pKa = 5.02 VYY406 pKa = 10.77 SPVHH410 pKa = 6.2 PGNN413 pKa = 3.48
Molecular weight: 43.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.675
IPC2_protein 3.719
IPC_protein 3.77
Toseland 3.528
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.745
Rodwell 3.592
Grimsley 3.439
Solomon 3.783
Lehninger 3.732
Nozaki 3.897
DTASelect 4.19
Thurlkill 3.592
EMBOSS 3.745
Sillero 3.897
Patrickios 1.074
IPC_peptide 3.77
IPC2_peptide 3.872
IPC2.peptide.svr19 3.814
Protein with the highest isoelectric point:
>tr|A0A1N7Q192|A0A1N7Q192_9GAMM Uncharacterized protein OS=Oleibacter marinus OX=484498 GN=SAMN05421686_11290 PE=4 SV=1
MM1 pKa = 7.65 DD2 pKa = 4.08 TQRR5 pKa = 11.84 LMALEE10 pKa = 4.53 GSWLEE15 pKa = 3.85 EE16 pKa = 3.83 RR17 pKa = 11.84 VSHH20 pKa = 6.49 LRR22 pKa = 11.84 GRR24 pKa = 11.84 HH25 pKa = 5.08 LLYY28 pKa = 10.88 AGVDD32 pKa = 3.61 TDD34 pKa = 4.01 PKK36 pKa = 10.44 FLGRR40 pKa = 11.84 SRR42 pKa = 11.84 ADD44 pKa = 2.91 HH45 pKa = 5.93 TFRR48 pKa = 11.84 VGLPWQKK55 pKa = 11.3 GLVDD59 pKa = 4.2 CQAQIDD65 pKa = 3.94 NTRR68 pKa = 11.84 WPFPDD73 pKa = 3.08 EE74 pKa = 4.07 TLDD77 pKa = 4.0 VVVLQHH83 pKa = 6.7 ALDD86 pKa = 3.84 MTSRR90 pKa = 11.84 PHH92 pKa = 5.66 QLVRR96 pKa = 11.84 EE97 pKa = 4.44 ATRR100 pKa = 11.84 SLVSGGYY107 pKa = 9.87 LVVVGFNPYY116 pKa = 8.36 STWGGVRR123 pKa = 11.84 WLRR126 pKa = 11.84 TMSTEE131 pKa = 4.33 LPWVSNPVAPLRR143 pKa = 11.84 LSDD146 pKa = 3.51 WLMLLDD152 pKa = 4.61 FRR154 pKa = 11.84 VEE156 pKa = 4.42 NITTAAHH163 pKa = 6.6 LWPVKK168 pKa = 10.1 IGSEE172 pKa = 3.96 AMSRR176 pKa = 11.84 RR177 pKa = 11.84 VDD179 pKa = 3.25 RR180 pKa = 11.84 VLAGNRR186 pKa = 11.84 MVAGNIYY193 pKa = 10.56 LLVARR198 pKa = 11.84 KK199 pKa = 7.18 TVAGMTTIRR208 pKa = 11.84 SGRR211 pKa = 11.84 KK212 pKa = 6.98 VRR214 pKa = 11.84 RR215 pKa = 11.84 EE216 pKa = 3.9 PKK218 pKa = 10.22 LGFAIPTTRR227 pKa = 11.84 KK228 pKa = 8.16 PVSRR232 pKa = 11.84 IKK234 pKa = 11.02 GSLL237 pKa = 3.22
Molecular weight: 26.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.34
IPC2_protein 9.633
IPC_protein 10.672
Toseland 10.818
ProMoST 10.701
Dawson 10.891
Bjellqvist 10.672
Wikipedia 11.169
Rodwell 10.95
Grimsley 10.935
Solomon 11.096
Lehninger 11.052
Nozaki 10.804
DTASelect 10.657
Thurlkill 10.818
EMBOSS 11.242
Sillero 10.833
Patrickios 10.672
IPC_peptide 11.111
IPC2_peptide 9.721
IPC2.peptide.svr19 8.759
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3470
0
3470
1171308
27
5033
337.6
37.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.425 ± 0.053
0.932 ± 0.016
6.417 ± 0.079
6.788 ± 0.046
3.748 ± 0.028
7.299 ± 0.042
2.082 ± 0.022
5.593 ± 0.029
4.092 ± 0.046
10.136 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.545 ± 0.024
3.752 ± 0.043
4.239 ± 0.034
3.968 ± 0.029
5.538 ± 0.047
6.729 ± 0.038
5.534 ± 0.06
7.021 ± 0.04
1.302 ± 0.017
2.863 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here