Enterospora canceri
Average proteome isoelectric point is 6.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2147 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y1S749|A0A1Y1S749_9MICR Uncharacterized protein OS=Enterospora canceri OX=1081671 GN=ECANGB1_971 PE=4 SV=1
MM1 pKa = 7.26 YY2 pKa = 10.61 SSFLLALLNLQIILAAEE19 pKa = 4.13 KK20 pKa = 10.88 KK21 pKa = 8.61 EE22 pKa = 4.27 TGDD25 pKa = 4.45 DD26 pKa = 3.42 ATADD30 pKa = 3.66 STKK33 pKa = 10.28 TEE35 pKa = 3.95 QQQTVTEE42 pKa = 4.32 GEE44 pKa = 4.19 KK45 pKa = 10.46 LDD47 pKa = 3.85 PSAIIGGRR55 pKa = 11.84 DD56 pKa = 3.78 FVCVKK61 pKa = 10.62 CLDD64 pKa = 3.87 NGVDD68 pKa = 3.6 SSSDD72 pKa = 3.33 ATKK75 pKa = 10.55 DD76 pKa = 3.81 CCDD79 pKa = 3.91 CCCEE83 pKa = 5.08 NDD85 pKa = 4.33 DD86 pKa = 4.93 PCDD89 pKa = 3.74 GTIKK93 pKa = 10.56 PNPPPCACLDD103 pKa = 3.51 EE104 pKa = 4.9 TGNIDD109 pKa = 3.93 PKK111 pKa = 10.59 RR112 pKa = 11.84 GYY114 pKa = 10.51
Molecular weight: 12.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.765
IPC2_protein 3.986
IPC_protein 3.961
Toseland 3.732
ProMoST 4.126
Dawson 3.961
Bjellqvist 4.113
Wikipedia 3.897
Rodwell 3.783
Grimsley 3.643
Solomon 3.948
Lehninger 3.897
Nozaki 4.075
DTASelect 4.329
Thurlkill 3.795
EMBOSS 3.91
Sillero 4.075
Patrickios 1.036
IPC_peptide 3.935
IPC2_peptide 4.05
IPC2.peptide.svr19 3.966
Protein with the highest isoelectric point:
>tr|A0A1Y1S4K0|A0A1Y1S4K0_9MICR SGNH_hydro domain-containing protein OS=Enterospora canceri OX=1081671 GN=ECANGB1_208 PE=4 SV=1
MM1 pKa = 7.37 LWEE4 pKa = 4.28 AWWWWWWWWWFRR16 pKa = 11.84 IHH18 pKa = 7.01 CGNKK22 pKa = 9.03 FCVHH26 pKa = 6.42 LKK28 pKa = 10.13 IPYY31 pKa = 9.16 KK32 pKa = 9.8 RR33 pKa = 11.84 QTYY36 pKa = 9.89 SGSRR40 pKa = 11.84 SDD42 pKa = 3.95 AIRR45 pKa = 11.84 RR46 pKa = 11.84 ALIGPAGSSLFFPDD60 pKa = 3.76 PAAGINIEE68 pKa = 3.78 KK69 pKa = 10.23 RR70 pKa = 11.84 RR71 pKa = 11.84 NLRR74 pKa = 11.84 VIQLVDD80 pKa = 3.92 RR81 pKa = 11.84 VACAAAAAAATTRR94 pKa = 11.84 HH95 pKa = 5.61 PQQTTTTTTTTTTASPPPSCLDD117 pKa = 3.22 VARR120 pKa = 11.84 VGKK123 pKa = 10.31
Molecular weight: 14.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.351
IPC2_protein 9.516
IPC_protein 10.101
Toseland 10.57
ProMoST 10.306
Dawson 10.657
Bjellqvist 10.365
Wikipedia 10.847
Rodwell 10.906
Grimsley 10.701
Solomon 10.76
Lehninger 10.745
Nozaki 10.599
DTASelect 10.35
Thurlkill 10.57
EMBOSS 10.965
Sillero 10.599
Patrickios 10.687
IPC_peptide 10.774
IPC2_peptide 9.663
IPC2.peptide.svr19 8.546
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2147
0
2147
604107
23
2685
281.4
32.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.026 ± 0.049
1.867 ± 0.027
5.332 ± 0.039
8.015 ± 0.065
4.759 ± 0.044
4.987 ± 0.051
1.964 ± 0.027
7.334 ± 0.06
8.671 ± 0.072
8.977 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.859 ± 0.029
5.878 ± 0.044
2.687 ± 0.04
3.147 ± 0.032
5.215 ± 0.048
6.618 ± 0.055
5.541 ± 0.042
6.528 ± 0.043
0.638 ± 0.014
3.944 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here