Alteromonas virus vB_AspP-H4/4
Average proteome isoelectric point is 6.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A220YL84|A0A220YL84_9CAUD Uncharacterized protein OS=Alteromonas virus vB_AspP-H4/4 OX=2016032 GN=vBAspPH44_49 PE=4 SV=1
MM1 pKa = 7.85 IINTILKK8 pKa = 9.88 DD9 pKa = 3.41 ISVLLLGVALSAAIIGLDD27 pKa = 3.03 IAADD31 pKa = 3.64 MVVNDD36 pKa = 3.51 YY37 pKa = 11.24 KK38 pKa = 10.97 EE39 pKa = 4.14 YY40 pKa = 10.1 QLEE43 pKa = 4.37 SACVAEE49 pKa = 4.7 YY50 pKa = 10.25 IAVGIEE56 pKa = 3.82 RR57 pKa = 11.84 KK58 pKa = 10.35 DD59 pKa = 3.4 IITSNGTCTVKK70 pKa = 10.58 EE71 pKa = 4.11 LL72 pKa = 3.94
Molecular weight: 7.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.216
IPC2_protein 4.329
IPC_protein 4.151
Toseland 3.973
ProMoST 4.291
Dawson 4.126
Bjellqvist 4.279
Wikipedia 4.037
Rodwell 3.986
Grimsley 3.897
Solomon 4.101
Lehninger 4.062
Nozaki 4.253
DTASelect 4.406
Thurlkill 4.024
EMBOSS 4.05
Sillero 4.266
Patrickios 3.198
IPC_peptide 4.101
IPC2_peptide 4.24
IPC2.peptide.svr19 4.181
Protein with the highest isoelectric point:
>tr|A0A220YL47|A0A220YL47_9CAUD Putative nucleotidyl transferase OS=Alteromonas virus vB_AspP-H4/4 OX=2016032 GN=vBAspPH44_9 PE=4 SV=1
MM1 pKa = 7.76 KK2 pKa = 10.34 SRR4 pKa = 11.84 SSRR7 pKa = 11.84 NFRR10 pKa = 11.84 QQGSVKK16 pKa = 10.39 AMKK19 pKa = 9.76 KK20 pKa = 10.25 AYY22 pKa = 8.93 RR23 pKa = 11.84 TEE25 pKa = 3.73 QRR27 pKa = 11.84 QDD29 pKa = 2.87 KK30 pKa = 8.86 TLTPQRR36 pKa = 11.84 SDD38 pKa = 3.01 AMIAGHH44 pKa = 5.91 EE45 pKa = 4.4 FIEE48 pKa = 4.67 AFLSRR53 pKa = 11.84 GKK55 pKa = 10.19 QVVVRR60 pKa = 11.84 AVV62 pKa = 2.71
Molecular weight: 7.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.229
IPC2_protein 9.984
IPC_protein 11.155
Toseland 11.403
ProMoST 11.696
Dawson 11.433
Bjellqvist 11.301
Wikipedia 11.798
Rodwell 11.506
Grimsley 11.462
Solomon 11.798
Lehninger 11.711
Nozaki 11.389
DTASelect 11.301
Thurlkill 11.389
EMBOSS 11.857
Sillero 11.389
Patrickios 11.257
IPC_peptide 11.798
IPC2_peptide 10.672
IPC2.peptide.svr19 9.045
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
49
0
49
15438
46
1956
315.1
35.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.213 ± 0.409
0.836 ± 0.142
6.827 ± 0.211
7.423 ± 0.249
3.55 ± 0.214
6.756 ± 0.338
1.885 ± 0.192
5.344 ± 0.232
6.549 ± 0.524
7.838 ± 0.18
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.818 ± 0.204
5.014 ± 0.245
3.407 ± 0.208
4.165 ± 0.322
5.13 ± 0.222
5.765 ± 0.177
6.303 ± 0.397
7.197 ± 0.228
1.386 ± 0.136
3.595 ± 0.168
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here