Microcystis aeruginosa Ma_AC_P_19900807_S299

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Oscillatoriophycideae; Chroococcales; Microcystaceae; Microcystis; Microcystis aeruginosa

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3434 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A552A7E3|A0A552A7E3_MICAE DNA polymerase subunit beta OS=Microcystis aeruginosa Ma_AC_P_19900807_S299 OX=2486264 GN=EWV82_13020 PE=4 SV=1
MM1 pKa = 7.56FDD3 pKa = 3.03STGIQLRR10 pKa = 11.84VSDD13 pKa = 5.48DD14 pKa = 3.51NPAPNEE20 pKa = 3.97PFSGDD25 pKa = 3.23SYY27 pKa = 11.7IDD29 pKa = 3.23FTASVSGTYY38 pKa = 9.56YY39 pKa = 10.72VGVSGYY45 pKa = 11.35SNFSYY50 pKa = 11.17NPFIDD55 pKa = 3.92GSGALSSSYY64 pKa = 11.32GDD66 pKa = 3.3YY67 pKa = 10.48TLEE70 pKa = 4.05IKK72 pKa = 10.62VGSGGSTNQVVLSEE86 pKa = 4.34PNNIISQAVDD96 pKa = 3.04SGLSSANQGIFIGTGSIRR114 pKa = 11.84TDD116 pKa = 3.22TNPITFFNGIITADD130 pKa = 3.52VGTLSSGEE138 pKa = 4.26SATIDD143 pKa = 3.43LTLSSIAAGNFNSTTIVTSNEE164 pKa = 3.53SDD166 pKa = 3.87ANPLDD171 pKa = 3.68NLIAGTQAVNSIAPAEE187 pKa = 4.23IDD189 pKa = 4.21LEE191 pKa = 4.27LTQTVNNSNPAIGDD205 pKa = 4.09EE206 pKa = 4.16ITLTLTLTNIGPGVASNIQVTNVLPLDD233 pKa = 3.55LAFISVVAEE242 pKa = 3.55QGTYY246 pKa = 10.91DD247 pKa = 4.13SNTGIWDD254 pKa = 3.61VGNMRR259 pKa = 11.84DD260 pKa = 3.62NLSRR264 pKa = 11.84TLTLTAQVNGGQSLTNTAQVTAVFEE289 pKa = 4.63ADD291 pKa = 4.32LDD293 pKa = 4.56SIPNNNDD300 pKa = 3.14CARR303 pKa = 11.84KK304 pKa = 9.4QNLNGFRR311 pKa = 11.84RR312 pKa = 11.84CVFYY316 pKa = 10.39FPQYY320 pKa = 10.05FYY322 pKa = 11.72ANRR325 pKa = 11.84WEE327 pKa = 4.05WW328 pKa = 3.01

Molecular weight: 34.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A551ZU75|A0A551ZU75_MICAE Glycogen debranching protein (Fragment) OS=Microcystis aeruginosa Ma_AC_P_19900807_S299 OX=2486264 GN=EWV82_20370 PE=4 SV=1
MM1 pKa = 7.79SKK3 pKa = 8.99RR4 pKa = 11.84TLEE7 pKa = 3.95GTTRR11 pKa = 11.84KK12 pKa = 9.34QKK14 pKa = 8.98RR15 pKa = 11.84TSGFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84SVNGRR29 pKa = 11.84KK30 pKa = 9.33VIKK33 pKa = 10.18ARR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 7.72RR38 pKa = 11.84GRR40 pKa = 11.84YY41 pKa = 8.57RR42 pKa = 11.84LSVV45 pKa = 3.14

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3434

0

3434

931635

21

3732

271.3

30.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.402 ± 0.056

1.03 ± 0.016

5.009 ± 0.034

6.555 ± 0.05

4.158 ± 0.031

6.462 ± 0.051

1.732 ± 0.025

7.295 ± 0.041

5.271 ± 0.057

11.208 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.707 ± 0.02

4.72 ± 0.049

4.589 ± 0.036

5.097 ± 0.044

5.021 ± 0.038

6.489 ± 0.037

5.545 ± 0.069

5.949 ± 0.038

1.464 ± 0.018

3.298 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski