Aythya fuligula (Tufted duck) (Anas fuligula)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria; Archosauria; Dinosauria; Saurischia;

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 24034 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6J3EPL9|A0A6J3EPL9_AYTFU glucose 1 6-bisphosphate synthase OS=Aythya fuligula OX=219594 GN=PGM2L1 PE=3 SV=1
MM1 pKa = 7.4TPNPSTTTTPDD12 pKa = 2.66ISTSTLVPSASTLTTPEE29 pKa = 3.57SSYY32 pKa = 9.68PTTPDD37 pKa = 2.87TSATTIPDD45 pKa = 3.56TSDD48 pKa = 2.73TTIPDD53 pKa = 3.6TSDD56 pKa = 2.77TTTPNASATMTPDD69 pKa = 3.13TSDD72 pKa = 2.9TTIPDD77 pKa = 3.7TSATMTPDD85 pKa = 3.11TSDD88 pKa = 2.9TTIPDD93 pKa = 3.56TSDD96 pKa = 2.49TMTPDD101 pKa = 3.6PSDD104 pKa = 3.23TTTPNASATMTPDD117 pKa = 3.13TSDD120 pKa = 2.9TTIPDD125 pKa = 3.56TSDD128 pKa = 2.36TMTPDD133 pKa = 3.17TSDD136 pKa = 2.9TTIPDD141 pKa = 3.56TSDD144 pKa = 2.52TMTPNASATMTPDD157 pKa = 3.09TSDD160 pKa = 2.61TMTPDD165 pKa = 3.12TSDD168 pKa = 2.98TTTPNASATMTPDD181 pKa = 3.13TSDD184 pKa = 2.9TTIPDD189 pKa = 3.6TSDD192 pKa = 2.75TTTPNVSATMTPDD205 pKa = 3.15TSDD208 pKa = 2.9TTIPDD213 pKa = 3.6TSDD216 pKa = 2.77TTTPNASATMTPDD229 pKa = 3.13TSDD232 pKa = 2.9TTIPDD237 pKa = 3.6TSDD240 pKa = 2.77TTTPNASATMTPDD253 pKa = 3.13TSDD256 pKa = 2.9TTIPDD261 pKa = 3.6TSDD264 pKa = 2.77TTTPNASATMTPDD277 pKa = 3.13TSDD280 pKa = 2.9TTIPDD285 pKa = 3.6TSDD288 pKa = 2.75TTTPNVSATMTPDD301 pKa = 3.15TSDD304 pKa = 2.9TTIPDD309 pKa = 3.6TSDD312 pKa = 2.77TTTPNASATMTPDD325 pKa = 3.13TSDD328 pKa = 2.9TTIPDD333 pKa = 3.6TSDD336 pKa = 2.77TTTPNASATMTPDD349 pKa = 3.13TSDD352 pKa = 2.9TTIPDD357 pKa = 3.6TSDD360 pKa = 2.75TTTPNVSATMTPDD373 pKa = 3.15TSDD376 pKa = 2.9TTIPDD381 pKa = 3.6TSDD384 pKa = 2.75TTTPNVSATMTPDD397 pKa = 3.15TSDD400 pKa = 2.9TTIPDD405 pKa = 3.56TSDD408 pKa = 2.52TMTPNASATMTPDD421 pKa = 3.13TSDD424 pKa = 2.9TTIPDD429 pKa = 3.6TSDD432 pKa = 2.77TTTPNASATMTPDD445 pKa = 3.15TSDD448 pKa = 3.08TTILDD453 pKa = 3.76TSATMTPDD461 pKa = 3.08TSDD464 pKa = 2.99TTTPEE469 pKa = 3.91PSATTTPDD477 pKa = 3.38PFDD480 pKa = 3.55TTTPDD485 pKa = 3.34PSATTTLDD493 pKa = 3.54PSDD496 pKa = 3.85TTTPDD501 pKa = 3.22PSATTTPDD509 pKa = 2.86SSTLTTPDD517 pKa = 3.73PSAMTTPDD525 pKa = 3.82PSTVTLDD532 pKa = 3.76PTNSTPTLDD541 pKa = 3.83PTTLTATPTSSSPASTPDD559 pKa = 3.0SSTSALTPTSSVSTSTLTSSVTPPTPDD586 pKa = 3.18SPSTPTSSIPTTTNITGSSALTTTSSTAPDD616 pKa = 3.31ISSTPTASSAATTSLVTTTTPSTTTSPTDD645 pKa = 3.06VTTTINSPMTTSGNTTSPTTTTMMTTGATNTTTLPTTTTTTTKK688 pKa = 9.18PTTTTTHH695 pKa = 5.52STTTTTTTTTTTTTTTTSTMSPTTTPTTTTPGVCEE730 pKa = 4.78NGGTWVNGQCVCPPGYY746 pKa = 9.2TGNTCSSIEE755 pKa = 3.98SSIEE759 pKa = 3.79TKK761 pKa = 10.92AEE763 pKa = 4.11TNVSVSVEE771 pKa = 3.68LRR773 pKa = 11.84VTNQEE778 pKa = 3.7FTEE781 pKa = 4.07DD782 pKa = 3.69LRR784 pKa = 11.84NEE786 pKa = 3.86SSKK789 pKa = 10.81GYY791 pKa = 10.33KK792 pKa = 10.13SFVLTFTLQMEE803 pKa = 4.68IYY805 pKa = 8.72YY806 pKa = 10.5RR807 pKa = 11.84NIEE810 pKa = 4.24GYY812 pKa = 9.96QGIKK816 pKa = 9.84ILSLSPGSIVVNHH829 pKa = 5.38TVIVAVPVTADD840 pKa = 3.34TVEE843 pKa = 4.44KK844 pKa = 10.35IGNVTKK850 pKa = 10.73NVEE853 pKa = 4.05KK854 pKa = 10.72EE855 pKa = 3.97IVQAAAQHH863 pKa = 5.63EE864 pKa = 4.67NCTEE868 pKa = 3.68NSTFCFNASEE878 pKa = 4.22VTIAQPSYY886 pKa = 9.96EE887 pKa = 4.28FNATEE892 pKa = 4.34YY893 pKa = 10.46CRR895 pKa = 11.84QVTPNGFKK903 pKa = 10.73DD904 pKa = 4.34FYY906 pKa = 11.33YY907 pKa = 10.45PNTTKK912 pKa = 10.9NGLTCVSNCSANTASAIDD930 pKa = 4.15CNEE933 pKa = 3.99GQCQLTLRR941 pKa = 11.84GPQCFCRR948 pKa = 11.84EE949 pKa = 4.08TNLYY953 pKa = 8.61WYY955 pKa = 10.49QGDD958 pKa = 3.73RR959 pKa = 11.84CSSRR963 pKa = 11.84VSKK966 pKa = 10.08MAVGMGLAVAVLCLVIFGLAAFIFRR991 pKa = 11.84ARR993 pKa = 11.84RR994 pKa = 11.84LKK996 pKa = 11.03LSDD999 pKa = 3.71RR1000 pKa = 11.84YY1001 pKa = 11.06SDD1003 pKa = 3.43LKK1005 pKa = 9.11EE1006 pKa = 4.46TKK1008 pKa = 8.36EE1009 pKa = 3.61QWYY1012 pKa = 10.27EE1013 pKa = 3.92EE1014 pKa = 4.1EE1015 pKa = 4.43DD1016 pKa = 4.27EE1017 pKa = 4.17NWKK1020 pKa = 10.91APGGFTIQNDD1030 pKa = 4.32GAHH1033 pKa = 7.05EE1034 pKa = 3.94DD1035 pKa = 3.6TMRR1038 pKa = 11.84VDD1040 pKa = 4.0LQSVDD1045 pKa = 3.36TSAKK1049 pKa = 9.78VPTSAATATSLSTNSTTMVPNTTTTMSPTTSINSSLPTTSNLTTSITSSPTSASSPTTISRR1110 pKa = 11.84PNTTTTRR1117 pKa = 11.84PTTTTTTTTTTTITTTTTTTPGVCEE1142 pKa = 4.78NGGTWVNGQCVCLPGYY1158 pKa = 9.26TGTTCSYY1165 pKa = 9.52VGSSIEE1171 pKa = 4.23TKK1173 pKa = 10.8AEE1175 pKa = 4.11TNVSVSVEE1183 pKa = 3.59LRR1185 pKa = 11.84VTNRR1189 pKa = 11.84EE1190 pKa = 3.67FTEE1193 pKa = 3.97DD1194 pKa = 3.27LRR1196 pKa = 11.84NEE1198 pKa = 3.71NSEE1201 pKa = 4.31GYY1203 pKa = 10.36KK1204 pKa = 10.54SFVHH1208 pKa = 6.32NFTLQMEE1215 pKa = 4.79IYY1217 pKa = 8.72YY1218 pKa = 10.5RR1219 pKa = 11.84NIEE1222 pKa = 4.24GYY1224 pKa = 9.96QGIKK1228 pKa = 9.85ILSLSNGSIVVNHH1241 pKa = 5.39TVIVAVPVTTDD1252 pKa = 3.16TVKK1255 pKa = 10.95KK1256 pKa = 9.77IDD1258 pKa = 3.75NVTKK1262 pKa = 10.15NVEE1265 pKa = 4.37EE1266 pKa = 4.16IVQAAAQQEE1275 pKa = 4.42NCTDD1279 pKa = 3.1NSTFCFNASEE1289 pKa = 4.22VTIAQPSYY1297 pKa = 10.22EE1298 pKa = 4.41FNATAYY1304 pKa = 8.7CHH1306 pKa = 5.75QVAPSDD1312 pKa = 3.96FKK1314 pKa = 11.46DD1315 pKa = 4.51FYY1317 pKa = 10.92YY1318 pKa = 10.47PNITKK1323 pKa = 10.5SGLTCVSNCSANTASAIDD1341 pKa = 4.15CNEE1344 pKa = 3.92GQCQLTHH1351 pKa = 6.91NGPQCLL1357 pKa = 3.73

Molecular weight: 141.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6J3DL10|A0A6J3DL10_AYTFU small integral membrane protein 4 OS=Aythya fuligula OX=219594 GN=SMIM4 PE=4 SV=1
MM1 pKa = 7.57SSHH4 pKa = 6.01KK5 pKa = 9.07TFKK8 pKa = 10.28IKK10 pKa = 10.6RR11 pKa = 11.84FLAKK15 pKa = 9.68KK16 pKa = 9.58QKK18 pKa = 8.69QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84MKK30 pKa = 9.89TGNKK34 pKa = 8.61IRR36 pKa = 11.84YY37 pKa = 7.09NSKK40 pKa = 8.3RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 3.95WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.83LGLL51 pKa = 3.67

Molecular weight: 6.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15477

8557

24034

17668086

31

33862

735.1

82.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.888 ± 0.016

2.05 ± 0.012

5.066 ± 0.009

7.48 ± 0.021

3.459 ± 0.01

6.142 ± 0.023

2.477 ± 0.006

4.557 ± 0.012

6.214 ± 0.02

9.401 ± 0.019

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.213 ± 0.007

3.968 ± 0.013

5.834 ± 0.022

4.897 ± 0.016

5.467 ± 0.014

8.603 ± 0.022

5.438 ± 0.014

6.031 ± 0.014

1.08 ± 0.005

2.731 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski