Proteome-pI 2.0 - Proteome Isoelectric Point Database

Phytophthora sojae (strain P6497) (Soybean stem and root rot agent) (Phytophthora megasperma f. sp. glycines)

Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae; Phytophthora; Phytophthora sojae

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 25721 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|G5AGU7|G5AGU7_PHYSP Uncharacterized protein OS=Phytophthora sojae (strain P6497) OX=1094619 GN=PHYSODRAFT_301636 PE=4 SV=1

MM1 pKa = 7.38GNAWCHH7 pKa = 6.41KK8 pKa = 10.11DD9 pKa = 3.55DD10 pKa = 4.91SPEE13 pKa = 4.54KK14 pKa = 10.56GDD16 pKa = 3.9DD17 pKa = 2.97RR18 pKa = 11.84VFRR21 pKa = 11.84NEE23 pKa = 4.14DD24 pKa = 3.51PPTEE28 pKa = 4.4GDD30 pKa = 4.53QEE32 pKa = 4.3DD33 pKa = 4.4TDD35 pKa = 5.03SDD37 pKa = 3.84EE38 pKa = 4.28QDD40 pKa = 3.22TRR42 pKa = 11.84DD43 pKa = 3.6AQAANNNRR51 pKa = 11.84NKK53 pKa = 10.38RR54 pKa = 11.84RR55 pKa = 11.84DD56 pKa = 3.75RR57 pKa = 11.84GLKK60 pKa = 9.59MYY62 pKa = 10.07DD63 pKa = 3.2SCDD66 pKa = 3.88DD67 pKa = 3.16VWMDD71 pKa = 5.2RR72 pKa = 11.84MWLYY76 pKa = 11.23DD77 pKa = 4.89LDD79 pKa = 4.65DD80 pKa = 3.94QQTPDD85 pKa = 3.7AVASGHH91 pKa = 7.17DD92 pKa = 3.62CGDD95 pKa = 3.42YY96 pKa = 11.29CGDD99 pKa = 3.52HH100 pKa = 6.92CGEE103 pKa = 5.08CDD105 pKa = 5.25LEE107 pKa = 4.39CHH109 pKa = 6.87DD110 pKa = 6.1APDD113 pKa = 4.85CSDD116 pKa = 6.19CGDD119 pKa = 4.05CWCDD123 pKa = 3.0

Molecular weight: 14.02 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|G5A417|G5A417_PHYSP Hexosyltransferase OS=Phytophthora sojae (strain P6497) OX=1094619 GN=PHYSODRAFT_318573 PE=3 SV=1

MM1 pKa = 7.55RR2 pKa = 11.84VSSAPKK8 pKa = 9.48VLSATRR14 pKa = 11.84APSAARR20 pKa = 11.84AVRR23 pKa = 11.84TGRR26 pKa = 11.84VPPATRR32 pKa = 11.84IPSTARR38 pKa = 11.84APSTARR44 pKa = 11.84APSAVRR50 pKa = 11.84VPSAA54 pKa = 3.48

Molecular weight: 5.46 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
25721	0	25721	9854020	49	16896	383.1	42.42

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
9.556 ± 0.021	1.71 ± 0.008	5.662 ± 0.013	6.401 ± 0.022	3.716 ± 0.013	6.333 ± 0.032	2.319 ± 0.007	3.949 ± 0.012	4.906 ± 0.017	9.336 ± 0.018

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.396 ± 0.008	3.347 ± 0.01	4.845 ± 0.014	4.099 ± 0.013	6.494 ± 0.02	8.068 ± 0.023	5.777 ± 0.018	7.142 ± 0.016	1.307 ± 0.006	2.64 ± 0.01

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski