Leptotrichia sp. oral taxon 215 str. W9775
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2260 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|U2RDX1|U2RDX1_9FUSO Uncharacterized protein OS=Leptotrichia sp. oral taxon 215 str. W9775 OX=1321779 GN=HMPREF1984_01355 PE=4 SV=1
MM1 pKa = 7.24 TKK3 pKa = 10.53 NEE5 pKa = 4.29 IANMTNGKK13 pKa = 9.6 LKK15 pKa = 10.93 GIARR19 pKa = 11.84 QISTNYY25 pKa = 10.07 EE26 pKa = 3.55 LGLRR30 pKa = 11.84 EE31 pKa = 4.31 DD32 pKa = 5.37 DD33 pKa = 4.94 IIILEE38 pKa = 4.38 KK39 pKa = 10.97 AEE41 pKa = 3.91 SWTDD45 pKa = 3.03 GGEE48 pKa = 3.93 FTVEE52 pKa = 3.62 NEE54 pKa = 3.78 RR55 pKa = 11.84 EE56 pKa = 4.03 YY57 pKa = 10.76 EE58 pKa = 4.18 YY59 pKa = 10.84 MFYY62 pKa = 10.54 CVNEE66 pKa = 4.4 CPVHH70 pKa = 7.15 IVDD73 pKa = 4.68 YY74 pKa = 10.32 EE75 pKa = 4.2 NEE77 pKa = 4.22 EE78 pKa = 3.9 EE79 pKa = 4.36 TEE81 pKa = 3.89 ILGATDD87 pKa = 4.31 CEE89 pKa = 4.41 AEE91 pKa = 4.24 KK92 pKa = 10.64 EE93 pKa = 4.4 VLVAAGTKK101 pKa = 9.82 FRR103 pKa = 11.84 IISVSTDD110 pKa = 2.62 EE111 pKa = 5.62 DD112 pKa = 4.37 YY113 pKa = 11.85 EE114 pKa = 4.32 EE115 pKa = 3.92 MGYY118 pKa = 11.13 YY119 pKa = 10.29 SIDD122 pKa = 3.42 VEE124 pKa = 4.55 YY125 pKa = 10.74 MNN127 pKa = 5.51
Molecular weight: 14.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.836
IPC2_protein 4.088
IPC_protein 3.999
Toseland 3.846
ProMoST 4.101
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.795
Rodwell 3.834
Grimsley 3.757
Solomon 3.935
Lehninger 3.884
Nozaki 4.05
DTASelect 4.151
Thurlkill 3.846
EMBOSS 3.808
Sillero 4.101
Patrickios 1.074
IPC_peptide 3.935
IPC2_peptide 4.101
IPC2.peptide.svr19 4.007
Protein with the highest isoelectric point:
>tr|U2RJ81|U2RJ81_9FUSO DAK2 domain fusion protein YloV OS=Leptotrichia sp. oral taxon 215 str. W9775 OX=1321779 GN=HMPREF1984_00401 PE=4 SV=1
MM1 pKa = 7.94 RR2 pKa = 11.84 KK3 pKa = 9.33 RR4 pKa = 11.84 RR5 pKa = 11.84 TLSGIGIAALFATGGAGNLFTAFVLMTISLTCLYY39 pKa = 10.08 FGKK42 pKa = 10.63 AFDD45 pKa = 3.87 RR46 pKa = 11.84 KK47 pKa = 7.21 TWEE50 pKa = 3.73
Molecular weight: 5.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.321
IPC2_protein 9.648
IPC_protein 10.204
Toseland 10.687
ProMoST 10.277
Dawson 10.774
Bjellqvist 10.438
Wikipedia 10.935
Rodwell 11.125
Grimsley 10.804
Solomon 10.877
Lehninger 10.847
Nozaki 10.672
DTASelect 10.423
Thurlkill 10.672
EMBOSS 11.067
Sillero 10.701
Patrickios 10.979
IPC_peptide 10.877
IPC2_peptide 9.443
IPC2.peptide.svr19 8.535
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2260
0
2260
690179
39
2995
305.4
34.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.413 ± 0.068
0.671 ± 0.017
5.24 ± 0.04
8.553 ± 0.1
4.773 ± 0.047
6.697 ± 0.093
1.271 ± 0.018
9.154 ± 0.057
10.04 ± 0.087
8.67 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.574 ± 0.026
6.375 ± 0.067
2.631 ± 0.027
2.224 ± 0.02
3.477 ± 0.035
5.833 ± 0.048
5.042 ± 0.078
6.346 ± 0.044
0.72 ± 0.018
4.297 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here