Aeromicrobium sp. Leaf289

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Aeromicrobium; unclassified Aeromicrobium

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3243 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S9RD88|A0A0S9RD88_9ACTN N-acetyltransferase domain-containing protein OS=Aeromicrobium sp. Leaf289 OX=1736324 GN=ASF37_02760 PE=4 SV=1
MM1 pKa = 8.11IPMFLLATHH10 pKa = 6.81RR11 pKa = 11.84RR12 pKa = 11.84SEE14 pKa = 4.08RR15 pKa = 11.84GQGSLEE21 pKa = 4.16TVGVVVLASILVAATTGVVVQSSPALRR48 pKa = 11.84SEE50 pKa = 3.7VGYY53 pKa = 9.72RR54 pKa = 11.84VCQITSLGGGGCEE67 pKa = 4.94DD68 pKa = 4.9PGDD71 pKa = 4.92EE72 pKa = 5.25IPGDD76 pKa = 4.13DD77 pKa = 4.73GADD80 pKa = 3.48PDD82 pKa = 4.92SPWGNEE88 pKa = 3.56NARR91 pKa = 11.84PDD93 pKa = 3.67GKK95 pKa = 9.94PAVDD99 pKa = 3.88EE100 pKa = 4.35SRR102 pKa = 11.84LPDD105 pKa = 3.68EE106 pKa = 4.76PCVVSSTSGSISATGSFVVSATGEE130 pKa = 3.71QGMTIEE136 pKa = 4.36TLSDD140 pKa = 2.89GTYY143 pKa = 9.81RR144 pKa = 11.84VSVHH148 pKa = 6.9DD149 pKa = 5.09AGSLGYY155 pKa = 10.4GVGIGVDD162 pKa = 3.64GNVVVDD168 pKa = 4.85GVQQGSTAYY177 pKa = 10.1AGADD181 pKa = 2.93IALAGLASQTYY192 pKa = 8.64YY193 pKa = 11.0AGSEE197 pKa = 4.09QEE199 pKa = 4.29LQDD202 pKa = 3.78LLVRR206 pKa = 11.84VAGEE210 pKa = 3.69QALDD214 pKa = 3.53QVAPRR219 pKa = 11.84IPGPADD225 pKa = 2.91IPFVQDD231 pKa = 2.95IPVIGEE237 pKa = 4.37LEE239 pKa = 4.06ALDD242 pKa = 4.32NPVRR246 pKa = 11.84WAVDD250 pKa = 3.38QLAGPPPEE258 pKa = 5.0PDD260 pKa = 3.01EE261 pKa = 5.19QYY263 pKa = 11.32VQLGLEE269 pKa = 4.44GDD271 pKa = 3.84ASAALAGIVYY281 pKa = 8.55STGAEE286 pKa = 3.66LDD288 pKa = 3.38MGVFAGARR296 pKa = 11.84TTPEE300 pKa = 4.51GYY302 pKa = 10.44VLTSVVEE309 pKa = 4.46ANGSAWASAFGGEE322 pKa = 4.1AQAMAGGTLVRR333 pKa = 11.84EE334 pKa = 4.12IHH336 pKa = 6.65LGPDD340 pKa = 3.66GTPLGITMTLTGGANAQSGDD360 pKa = 3.74VGPATDD366 pKa = 3.63TQDD369 pKa = 3.43SYY371 pKa = 12.13VATAYY376 pKa = 10.79VPFTGDD382 pKa = 2.68VATDD386 pKa = 3.16AGIYY390 pKa = 10.16LSAANMYY397 pKa = 8.03QAPQFLQSAMEE408 pKa = 4.24TGTVSLDD415 pKa = 3.42QYY417 pKa = 11.18EE418 pKa = 4.48VDD420 pKa = 3.71PNTYY424 pKa = 9.26GANVSIDD431 pKa = 3.17IPGAEE436 pKa = 4.64GGLGVNGDD444 pKa = 4.11LTNSTMVSSSYY455 pKa = 10.0WDD457 pKa = 3.15GSGFVDD463 pKa = 5.01RR464 pKa = 11.84GCC466 pKa = 4.37

Molecular weight: 47.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S9R810|A0A0S9R810_9ACTN Uncharacterized protein OS=Aeromicrobium sp. Leaf289 OX=1736324 GN=ASF37_06645 PE=4 SV=1
MM1 pKa = 7.74SKK3 pKa = 8.93RR4 pKa = 11.84TYY6 pKa = 10.0QPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.97KK16 pKa = 9.33HH17 pKa = 4.25GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84SILANRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.83GRR40 pKa = 11.84AKK42 pKa = 10.68LSAA45 pKa = 3.92

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3243

0

3243

1030146

37

3044

317.7

33.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.962 ± 0.052

0.653 ± 0.01

6.91 ± 0.039

5.761 ± 0.041

2.769 ± 0.025

9.163 ± 0.039

2.172 ± 0.022

3.219 ± 0.032

1.935 ± 0.033

10.21 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.695 ± 0.018

1.609 ± 0.022

5.41 ± 0.032

2.74 ± 0.022

7.672 ± 0.051

5.426 ± 0.031

6.224 ± 0.041

10.154 ± 0.046

1.471 ± 0.021

1.845 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski