Amino acid dipepetide frequency for Aeromicrobium sp. Leaf289

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.723AlaAla: 18.723 ± 0.198
0.942AlaCys: 0.942 ± 0.033
9.148AlaAsp: 9.148 ± 0.101
7.876AlaGlu: 7.876 ± 0.14
3.711AlaPhe: 3.711 ± 0.07
11.688AlaGly: 11.688 ± 0.13
2.517AlaHis: 2.517 ± 0.055
4.046AlaIle: 4.046 ± 0.074
2.542AlaLys: 2.542 ± 0.075
13.752AlaLeu: 13.752 ± 0.139
2.598AlaMet: 2.598 ± 0.051
1.821AlaAsn: 1.821 ± 0.042
6.001AlaPro: 6.001 ± 0.089
3.71AlaGln: 3.71 ± 0.064
9.361AlaArg: 9.361 ± 0.113
6.799AlaSer: 6.799 ± 0.088
7.613AlaThr: 7.613 ± 0.099
12.445AlaVal: 12.445 ± 0.138
2.004AlaTrp: 2.004 ± 0.046
2.323AlaTyr: 2.323 ± 0.054
0.0AlaXaa: 0.0 ± 0.0
Cys
0.779CysAla: 0.779 ± 0.028
0.053CysCys: 0.053 ± 0.007
0.431CysAsp: 0.431 ± 0.02
0.343CysGlu: 0.343 ± 0.016
0.202CysPhe: 0.202 ± 0.015
0.793CysGly: 0.793 ± 0.029
0.154CysHis: 0.154 ± 0.014
0.186CysIle: 0.186 ± 0.015
0.097CysLys: 0.097 ± 0.011
0.59CysLeu: 0.59 ± 0.024
0.1CysMet: 0.1 ± 0.011
0.115CysAsn: 0.115 ± 0.011
0.384CysPro: 0.384 ± 0.021
0.145CysGln: 0.145 ± 0.013
0.475CysArg: 0.475 ± 0.022
0.425CysSer: 0.425 ± 0.022
0.476CysThr: 0.476 ± 0.021
0.572CysVal: 0.572 ± 0.024
0.094CysTrp: 0.094 ± 0.01
0.114CysTyr: 0.114 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
9.441AspAla: 9.441 ± 0.107
0.315AspCys: 0.315 ± 0.02
5.715AspAsp: 5.715 ± 0.097
4.853AspGlu: 4.853 ± 0.093
1.599AspPhe: 1.599 ± 0.036
6.896AspGly: 6.896 ± 0.092
1.644AspHis: 1.644 ± 0.041
1.659AspIle: 1.659 ± 0.046
0.977AspLys: 0.977 ± 0.036
7.646AspLeu: 7.646 ± 0.085
0.821AspMet: 0.821 ± 0.029
0.789AspAsn: 0.789 ± 0.032
4.545AspPro: 4.545 ± 0.075
1.704AspGln: 1.704 ± 0.041
5.133AspArg: 5.133 ± 0.077
2.311AspSer: 2.311 ± 0.054
3.08AspThr: 3.08 ± 0.053
7.967AspVal: 7.967 ± 0.095
0.897AspTrp: 0.897 ± 0.031
1.102AspTyr: 1.102 ± 0.038
0.001AspXaa: 0.001 ± 0.001
Glu
7.215GluAla: 7.215 ± 0.111
0.311GluCys: 0.311 ± 0.017
3.116GluAsp: 3.116 ± 0.064
3.034GluGlu: 3.034 ± 0.062
1.283GluPhe: 1.283 ± 0.039
4.496GluGly: 4.496 ± 0.069
1.767GluHis: 1.767 ± 0.048
2.278GluIle: 2.278 ± 0.055
1.437GluLys: 1.437 ± 0.035
6.228GluLeu: 6.228 ± 0.089
0.884GluMet: 0.884 ± 0.029
0.995GluAsn: 0.995 ± 0.033
3.456GluPro: 3.456 ± 0.066
2.441GluGln: 2.441 ± 0.055
5.547GluArg: 5.547 ± 0.086
2.87GluSer: 2.87 ± 0.059
3.036GluThr: 3.036 ± 0.056
5.554GluVal: 5.554 ± 0.084
0.671GluTrp: 0.671 ± 0.026
0.985GluTyr: 0.985 ± 0.033
0.0GluXaa: 0.0 ± 0.0
Phe
3.732PheAla: 3.732 ± 0.07
0.256PheCys: 0.256 ± 0.016
2.182PheAsp: 2.182 ± 0.046
1.607PheGlu: 1.607 ± 0.036
0.9PhePhe: 0.9 ± 0.036
3.042PheGly: 3.042 ± 0.065
0.569PheHis: 0.569 ± 0.024
0.749PheIle: 0.749 ± 0.032
0.469PheLys: 0.469 ± 0.025
2.488PheLeu: 2.488 ± 0.052
0.43PheMet: 0.43 ± 0.02
0.481PheAsn: 0.481 ± 0.022
1.202PhePro: 1.202 ± 0.029
0.607PheGln: 0.607 ± 0.023
1.656PheArg: 1.656 ± 0.038
1.499PheSer: 1.499 ± 0.039
1.915PheThr: 1.915 ± 0.044
2.899PheVal: 2.899 ± 0.063
0.399PheTrp: 0.399 ± 0.024
0.611PheTyr: 0.611 ± 0.024
0.0PheXaa: 0.0 ± 0.0
Gly
10.611GlyAla: 10.611 ± 0.12
0.723GlyCys: 0.723 ± 0.028
5.648GlyAsp: 5.648 ± 0.074
4.931GlyGlu: 4.931 ± 0.077
3.046GlyPhe: 3.046 ± 0.062
8.01GlyGly: 8.01 ± 0.125
2.043GlyHis: 2.043 ± 0.048
3.717GlyIle: 3.717 ± 0.074
2.016GlyLys: 2.016 ± 0.045
9.293GlyLeu: 9.293 ± 0.11
1.748GlyMet: 1.748 ± 0.045
1.459GlyAsn: 1.459 ± 0.043
4.521GlyPro: 4.521 ± 0.062
2.455GlyGln: 2.455 ± 0.053
7.282GlyArg: 7.282 ± 0.092
5.889GlySer: 5.889 ± 0.082
6.07GlyThr: 6.07 ± 0.091
8.432GlyVal: 8.432 ± 0.118
1.627GlyTrp: 1.627 ± 0.044
2.111GlyTyr: 2.111 ± 0.047
0.0GlyXaa: 0.0 ± 0.0
His
2.634HisAla: 2.634 ± 0.053
0.149HisCys: 0.149 ± 0.012
1.712HisAsp: 1.712 ± 0.042
1.287HisGlu: 1.287 ± 0.039
0.524HisPhe: 0.524 ± 0.021
2.259HisGly: 2.259 ± 0.055
0.684HisHis: 0.684 ± 0.029
0.396HisIle: 0.396 ± 0.021
0.248HisLys: 0.248 ± 0.013
2.41HisLeu: 2.41 ± 0.053
0.254HisMet: 0.254 ± 0.016
0.289HisAsn: 0.289 ± 0.018
1.636HisPro: 1.636 ± 0.04
0.579HisGln: 0.579 ± 0.024
1.836HisArg: 1.836 ± 0.047
0.796HisSer: 0.796 ± 0.03
1.001HisThr: 1.001 ± 0.035
2.343HisVal: 2.343 ± 0.058
0.271HisTrp: 0.271 ± 0.017
0.409HisTyr: 0.409 ± 0.019
0.0HisXaa: 0.0 ± 0.0
Ile
4.603IleAla: 4.603 ± 0.088
0.251IleCys: 0.251 ± 0.019
2.822IleAsp: 2.822 ± 0.053
2.245IleGlu: 2.245 ± 0.047
0.748IlePhe: 0.748 ± 0.031
3.728IleGly: 3.728 ± 0.072
0.591IleHis: 0.591 ± 0.027
0.99IleIle: 0.99 ± 0.04
0.669IleLys: 0.669 ± 0.026
2.374IleLeu: 2.374 ± 0.06
0.441IleMet: 0.441 ± 0.021
0.676IleAsn: 0.676 ± 0.025
1.489IlePro: 1.489 ± 0.04
0.68IleGln: 0.68 ± 0.028
1.851IleArg: 1.851 ± 0.042
1.749IleSer: 1.749 ± 0.041
2.073IleThr: 2.073 ± 0.044
3.339IleVal: 3.339 ± 0.066
0.354IleTrp: 0.354 ± 0.02
0.514IleTyr: 0.514 ± 0.024
0.0IleXaa: 0.0 ± 0.0
Lys
2.717LysAla: 2.717 ± 0.07
0.067LysCys: 0.067 ± 0.008
1.298LysAsp: 1.298 ± 0.043
0.97LysGlu: 0.97 ± 0.035
0.387LysPhe: 0.387 ± 0.02
1.675LysGly: 1.675 ± 0.056
0.416LysHis: 0.416 ± 0.022
0.843LysIle: 0.843 ± 0.033
0.859LysLys: 0.859 ± 0.05
1.573LysLeu: 1.573 ± 0.041
0.307LysMet: 0.307 ± 0.02
0.487LysAsn: 0.487 ± 0.024
1.111LysPro: 1.111 ± 0.037
0.621LysGln: 0.621 ± 0.027
1.321LysArg: 1.321 ± 0.042
1.006LysSer: 1.006 ± 0.039
1.144LysThr: 1.144 ± 0.045
1.99LysVal: 1.99 ± 0.047
0.175LysTrp: 0.175 ± 0.013
0.381LysTyr: 0.381 ± 0.018
0.0LysXaa: 0.0 ± 0.0
Leu
14.546LeuAla: 14.546 ± 0.152
0.649LeuCys: 0.649 ± 0.024
7.687LeuAsp: 7.687 ± 0.096
5.86LeuGlu: 5.86 ± 0.081
2.44LeuPhe: 2.44 ± 0.054
9.702LeuGly: 9.702 ± 0.111
2.046LeuHis: 2.046 ± 0.051
2.291LeuIle: 2.291 ± 0.06
1.735LeuLys: 1.735 ± 0.053
10.561LeuLeu: 10.561 ± 0.139
1.535LeuMet: 1.535 ± 0.037
1.447LeuAsn: 1.447 ± 0.041
5.556LeuPro: 5.556 ± 0.081
2.567LeuGln: 2.567 ± 0.052
7.852LeuArg: 7.852 ± 0.108
5.215LeuSer: 5.215 ± 0.077
6.214LeuThr: 6.214 ± 0.071
11.566LeuVal: 11.566 ± 0.141
1.185LeuTrp: 1.185 ± 0.035
1.447LeuTyr: 1.447 ± 0.036
0.0LeuXaa: 0.0 ± 0.0
Met
2.148MetAla: 2.148 ± 0.048
0.096MetCys: 0.096 ± 0.011
0.865MetAsp: 0.865 ± 0.031
0.673MetGlu: 0.673 ± 0.025
0.462MetPhe: 0.462 ± 0.02
1.285MetGly: 1.285 ± 0.041
0.363MetHis: 0.363 ± 0.019
0.645MetIle: 0.645 ± 0.027
0.41MetLys: 0.41 ± 0.019
1.71MetLeu: 1.71 ± 0.046
0.25MetMet: 0.25 ± 0.016
0.34MetAsn: 0.34 ± 0.018
0.953MetPro: 0.953 ± 0.03
0.493MetGln: 0.493 ± 0.019
1.345MetArg: 1.345 ± 0.034
1.438MetSer: 1.438 ± 0.04
1.684MetThr: 1.684 ± 0.044
1.377MetVal: 1.377 ± 0.039
0.171MetTrp: 0.171 ± 0.014
0.245MetTyr: 0.245 ± 0.015
0.0MetXaa: 0.0 ± 0.0
Asn
2.026AsnAla: 2.026 ± 0.048
0.106AsnCys: 0.106 ± 0.011
1.009AsnAsp: 1.009 ± 0.034
0.75AsnGlu: 0.75 ± 0.028
0.46AsnPhe: 0.46 ± 0.02
1.548AsnGly: 1.548 ± 0.046
0.345AsnHis: 0.345 ± 0.015
0.636AsnIle: 0.636 ± 0.024
0.299AsnLys: 0.299 ± 0.017
1.716AsnLeu: 1.716 ± 0.043
0.238AsnMet: 0.238 ± 0.015
0.399AsnAsn: 0.399 ± 0.025
1.253AsnPro: 1.253 ± 0.037
0.487AsnGln: 0.487 ± 0.025
1.139AsnArg: 1.139 ± 0.034
0.659AsnSer: 0.659 ± 0.027
0.945AsnThr: 0.945 ± 0.04
1.535AsnVal: 1.535 ± 0.038
0.196AsnTrp: 0.196 ± 0.015
0.347AsnTyr: 0.347 ± 0.019
0.0AsnXaa: 0.0 ± 0.0
Pro
6.64ProAla: 6.64 ± 0.092
0.264ProCys: 0.264 ± 0.013
4.707ProAsp: 4.707 ± 0.07
3.832ProGlu: 3.832 ± 0.064
1.615ProPhe: 1.615 ± 0.04
5.382ProGly: 5.382 ± 0.078
1.133ProHis: 1.133 ± 0.034
1.51ProIle: 1.51 ± 0.041
0.991ProLys: 0.991 ± 0.034
4.709ProLeu: 4.709 ± 0.076
0.921ProMet: 0.921 ± 0.031
0.762ProAsn: 0.762 ± 0.026
2.434ProPro: 2.434 ± 0.062
1.438ProGln: 1.438 ± 0.034
3.709ProArg: 3.709 ± 0.064
3.227ProSer: 3.227 ± 0.066
3.696ProThr: 3.696 ± 0.083
5.123ProVal: 5.123 ± 0.073
0.979ProTrp: 0.979 ± 0.036
1.029ProTyr: 1.029 ± 0.029
0.0ProXaa: 0.0 ± 0.0
Gln
3.514GlnAla: 3.514 ± 0.061
0.137GlnCys: 0.137 ± 0.012
1.423GlnAsp: 1.423 ± 0.039
1.395GlnGlu: 1.395 ± 0.039
0.661GlnPhe: 0.661 ± 0.026
2.218GlnGly: 2.218 ± 0.05
0.72GlnHis: 0.72 ± 0.025
1.012GlnIle: 1.012 ± 0.034
0.657GlnLys: 0.657 ± 0.027
2.954GlnLeu: 2.954 ± 0.06
0.514GlnMet: 0.514 ± 0.02
0.511GlnAsn: 0.511 ± 0.026
1.37GlnPro: 1.37 ± 0.04
1.084GlnGln: 1.084 ± 0.038
2.576GlnArg: 2.576 ± 0.055
1.226GlnSer: 1.226 ± 0.036
1.423GlnThr: 1.423 ± 0.042
3.037GlnVal: 3.037 ± 0.048
0.424GlnTrp: 0.424 ± 0.02
0.543GlnTyr: 0.543 ± 0.023
0.0GlnXaa: 0.0 ± 0.0
Arg
8.78ArgAla: 8.78 ± 0.103
0.452ArgCys: 0.452 ± 0.025
4.818ArgAsp: 4.818 ± 0.07
4.429ArgGlu: 4.429 ± 0.073
2.348ArgPhe: 2.348 ± 0.045
5.757ArgGly: 5.757 ± 0.067
1.754ArgHis: 1.754 ± 0.042
2.985ArgIle: 2.985 ± 0.058
1.338ArgLys: 1.338 ± 0.043
8.224ArgLeu: 8.224 ± 0.116
1.645ArgMet: 1.645 ± 0.044
1.195ArgAsn: 1.195 ± 0.036
4.307ArgPro: 4.307 ± 0.07
2.062ArgGln: 2.062 ± 0.043
7.578ArgArg: 7.578 ± 0.12
4.561ArgSer: 4.561 ± 0.063
5.019ArgThr: 5.019 ± 0.068
6.478ArgVal: 6.478 ± 0.083
1.383ArgTrp: 1.383 ± 0.039
1.603ArgTyr: 1.603 ± 0.041
0.0ArgXaa: 0.0 ± 0.0
Ser
6.673SerAla: 6.673 ± 0.084
0.368SerCys: 0.368 ± 0.021
3.297SerAsp: 3.297 ± 0.06
2.449SerGlu: 2.449 ± 0.044
1.708SerPhe: 1.708 ± 0.037
5.737SerGly: 5.737 ± 0.092
0.98SerHis: 0.98 ± 0.033
1.987SerIle: 1.987 ± 0.05
0.97SerLys: 0.97 ± 0.039
5.149SerLeu: 5.149 ± 0.074
1.231SerMet: 1.231 ± 0.037
0.935SerAsn: 0.935 ± 0.031
2.926SerPro: 2.926 ± 0.053
1.406SerGln: 1.406 ± 0.045
3.939SerArg: 3.939 ± 0.059
3.536SerSer: 3.536 ± 0.07
4.011SerThr: 4.011 ± 0.077
4.939SerVal: 4.939 ± 0.074
0.914SerTrp: 0.914 ± 0.028
1.104SerTyr: 1.104 ± 0.038
0.0SerXaa: 0.0 ± 0.0
Thr
7.482ThrAla: 7.482 ± 0.097
0.443ThrCys: 0.443 ± 0.026
3.771ThrAsp: 3.771 ± 0.061
2.971ThrGlu: 2.971 ± 0.051
2.04ThrPhe: 2.04 ± 0.042
5.854ThrGly: 5.854 ± 0.085
1.183ThrHis: 1.183 ± 0.036
2.405ThrIle: 2.405 ± 0.055
1.228ThrLys: 1.228 ± 0.042
6.141ThrLeu: 6.141 ± 0.082
1.0ThrMet: 1.0 ± 0.032
1.052ThrAsn: 1.052 ± 0.038
3.841ThrPro: 3.841 ± 0.074
1.526ThrGln: 1.526 ± 0.039
3.946ThrArg: 3.946 ± 0.064
4.11ThrSer: 4.11 ± 0.066
4.373ThrThr: 4.373 ± 0.089
6.371ThrVal: 6.371 ± 0.086
1.078ThrTrp: 1.078 ± 0.033
1.423ThrTyr: 1.423 ± 0.044
0.0ThrXaa: 0.0 ± 0.0
Val
13.475ValAla: 13.475 ± 0.139
0.673ValCys: 0.673 ± 0.024
7.18ValAsp: 7.18 ± 0.092
6.437ValGlu: 6.437 ± 0.097
2.408ValPhe: 2.408 ± 0.051
8.655ValGly: 8.655 ± 0.113
2.164ValHis: 2.164 ± 0.056
2.842ValIle: 2.842 ± 0.07
1.795ValLys: 1.795 ± 0.048
10.972ValLeu: 10.972 ± 0.126
1.539ValMet: 1.539 ± 0.035
1.673ValAsn: 1.673 ± 0.038
5.471ValPro: 5.471 ± 0.078
2.416ValGln: 2.416 ± 0.048
7.404ValArg: 7.404 ± 0.102
4.962ValSer: 4.962 ± 0.069
6.386ValThr: 6.386 ± 0.089
12.404ValVal: 12.404 ± 0.15
1.188ValTrp: 1.188 ± 0.034
1.495ValTyr: 1.495 ± 0.041
0.001ValXaa: 0.001 ± 0.001
Trp
1.516TrpAla: 1.516 ± 0.04
0.134TrpCys: 0.134 ± 0.011
0.87TrpAsp: 0.87 ± 0.029
0.608TrpGlu: 0.608 ± 0.025
0.543TrpPhe: 0.543 ± 0.025
1.051TrpGly: 1.051 ± 0.031
0.338TrpHis: 0.338 ± 0.018
0.591TrpIle: 0.591 ± 0.026
0.315TrpLys: 0.315 ± 0.02
1.631TrpLeu: 1.631 ± 0.039
0.287TrpMet: 0.287 ± 0.016
0.35TrpAsn: 0.35 ± 0.02
0.743TrpPro: 0.743 ± 0.028
0.503TrpGln: 0.503 ± 0.023
1.229TrpArg: 1.229 ± 0.038
1.023TrpSer: 1.023 ± 0.036
1.056TrpThr: 1.056 ± 0.036
1.251TrpVal: 1.251 ± 0.032
0.386TrpTrp: 0.386 ± 0.022
0.288TrpTyr: 0.288 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.364TyrAla: 2.364 ± 0.049
0.138TyrCys: 0.138 ± 0.011
1.397TyrAsp: 1.397 ± 0.035
1.055TyrGlu: 1.055 ± 0.028
0.605TyrPhe: 0.605 ± 0.022
1.858TyrGly: 1.858 ± 0.048
0.303TyrHis: 0.303 ± 0.017
0.419TyrIle: 0.419 ± 0.019
0.298TyrLys: 0.298 ± 0.018
1.981TyrLeu: 1.981 ± 0.049
0.21TyrMet: 0.21 ± 0.013
0.315TyrAsn: 0.315 ± 0.017
0.902TyrPro: 0.902 ± 0.034
0.477TyrGln: 0.477 ± 0.022
1.538TyrArg: 1.538 ± 0.042
0.954TyrSer: 0.954 ± 0.033
1.022TyrThr: 1.022 ± 0.036
1.918TyrVal: 1.918 ± 0.046
0.317TyrTrp: 0.317 ± 0.017
0.381TyrTyr: 0.381 ± 0.018
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.002XaaSer: 0.002 ± 0.002
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3243 proteins (1030146 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski