Muricauda pacifica
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4085 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2T0M905|A0A2T0M905_9FLAO CubicO group peptidase (Beta-lactamase class C family) OS=Muricauda pacifica OX=1080225 GN=CLV81_2352 PE=4 SV=1
MM1 pKa = 7.44 EE2 pKa = 4.84 MSAQNSGDD10 pKa = 3.74 CRR12 pKa = 11.84 TAIPVCADD20 pKa = 3.43 MPILSATDD28 pKa = 3.77 GSGDD32 pKa = 3.86 IDD34 pKa = 5.58 DD35 pKa = 5.54 FDD37 pKa = 6.76 PDD39 pKa = 4.14 VITQTGCLEE48 pKa = 4.3 KK49 pKa = 11.05 GSVASANIEE58 pKa = 4.33 NNTSWYY64 pKa = 9.72 VFRR67 pKa = 11.84 AGTDD71 pKa = 3.48 GQIGFDD77 pKa = 3.57 IEE79 pKa = 4.38 ALPVTPGGIVTSEE92 pKa = 3.8 IDD94 pKa = 3.28 FALYY98 pKa = 10.7 GPFDD102 pKa = 3.51 QTLGSNFCTTIGNGSDD118 pKa = 2.81 IPIRR122 pKa = 11.84 CNYY125 pKa = 8.87 EE126 pKa = 3.57 INDD129 pKa = 3.83 TGFTGIGVNPISGQVGAPFVRR150 pKa = 11.84 ASQNTYY156 pKa = 10.99 DD157 pKa = 3.49 EE158 pKa = 4.42 WLDD161 pKa = 3.62 VQQGEE166 pKa = 4.4 IYY168 pKa = 10.92 YY169 pKa = 10.54 LFINNFNTNFNGDD182 pKa = 4.1 PEE184 pKa = 4.56 PFSLTFTGSSVDD196 pKa = 3.44 ADD198 pKa = 3.46 QNTALDD204 pKa = 3.73 CTLRR208 pKa = 11.84 DD209 pKa = 3.55 EE210 pKa = 5.33 FLGLDD215 pKa = 4.04 IIACEE220 pKa = 4.1 GDD222 pKa = 3.41 PDD224 pKa = 4.1 IVLSALNSPAGPNIANITWEE244 pKa = 4.17 VDD246 pKa = 3.43 LEE248 pKa = 4.43 DD249 pKa = 5.42 DD250 pKa = 4.2 GVYY253 pKa = 10.77 DD254 pKa = 4.03 QVLATGAGATEE265 pKa = 4.29 LTVTSPNSGRR275 pKa = 11.84 YY276 pKa = 8.68 RR277 pKa = 11.84 VTIEE281 pKa = 3.88 TTFATIITDD290 pKa = 4.41 DD291 pKa = 3.32 ILITFFGVPVLDD303 pKa = 3.92 EE304 pKa = 4.23 VRR306 pKa = 11.84 VLDD309 pKa = 4.85 DD310 pKa = 5.17 LVNSDD315 pKa = 3.35 QTDD318 pKa = 3.73 PYY320 pKa = 10.47 NIEE323 pKa = 3.73 ISVLGNGNYY332 pKa = 9.99 EE333 pKa = 3.88 YY334 pKa = 10.41 AINGGEE340 pKa = 4.09 FQDD343 pKa = 4.94 DD344 pKa = 4.14 PVFLDD349 pKa = 3.73 VPPGVNTVVINDD361 pKa = 3.43 VNGCGTTEE369 pKa = 4.51 PIEE372 pKa = 4.07 FLVVGYY378 pKa = 9.34 PKK380 pKa = 10.71 FFTPNGDD387 pKa = 3.92 GIHH390 pKa = 7.23 DD391 pKa = 3.74 NWNVLGVEE399 pKa = 4.3 TLTNPSIFIFDD410 pKa = 3.74 RR411 pKa = 11.84 YY412 pKa = 11.07 GKK414 pKa = 9.85 FLKK417 pKa = 10.63 QLDD420 pKa = 4.08 INSLGWDD427 pKa = 3.44 GTFNGRR433 pKa = 11.84 PLPSSDD439 pKa = 2.51 YY440 pKa = 10.38 WFRR443 pKa = 11.84 LDD445 pKa = 4.67 YY446 pKa = 11.16 EE447 pKa = 4.66 RR448 pKa = 11.84 DD449 pKa = 3.65 DD450 pKa = 4.16 EE451 pKa = 4.65 GVLVARR457 pKa = 11.84 SVRR460 pKa = 11.84 RR461 pKa = 11.84 HH462 pKa = 5.32 FSLIRR467 pKa = 4.08
Molecular weight: 50.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.696
IPC2_protein 3.63
IPC_protein 3.668
Toseland 3.427
ProMoST 3.834
Dawson 3.668
Bjellqvist 3.821
Wikipedia 3.617
Rodwell 3.49
Grimsley 3.338
Solomon 3.656
Lehninger 3.617
Nozaki 3.77
DTASelect 4.05
Thurlkill 3.49
EMBOSS 3.617
Sillero 3.795
Patrickios 1.049
IPC_peptide 3.656
IPC2_peptide 3.77
IPC2.peptide.svr19 3.712
Protein with the highest isoelectric point:
>tr|A0A2T0MJ46|A0A2T0MJ46_9FLAO Uncharacterized protein OS=Muricauda pacifica OX=1080225 GN=CLV81_1581 PE=4 SV=1
MM1 pKa = 7.07 TLQRR5 pKa = 11.84 FRR7 pKa = 11.84 ITFSGEE13 pKa = 3.68 ANKK16 pKa = 9.72 PLPIQIKK23 pKa = 8.91 PNVVKK28 pKa = 10.74 ILGRR32 pKa = 11.84 LGLYY36 pKa = 9.91 KK37 pKa = 10.37 ISPLRR42 pKa = 11.84 IYY44 pKa = 10.97 VVGCPFLFVV53 pKa = 3.5
Molecular weight: 6.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.316
IPC2_protein 10.014
IPC_protein 10.701
Toseland 10.847
ProMoST 10.496
Dawson 10.935
Bjellqvist 10.628
Wikipedia 11.125
Rodwell 11.301
Grimsley 10.994
Solomon 11.038
Lehninger 11.008
Nozaki 10.818
DTASelect 10.628
Thurlkill 10.833
EMBOSS 11.242
Sillero 10.862
Patrickios 11.096
IPC_peptide 11.052
IPC2_peptide 9.633
IPC2.peptide.svr19 8.214
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4085
0
4085
1342224
29
4767
328.6
37.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.214 ± 0.035
0.722 ± 0.011
5.631 ± 0.035
6.94 ± 0.035
5.404 ± 0.033
6.717 ± 0.036
1.826 ± 0.02
7.32 ± 0.032
7.189 ± 0.056
9.693 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.254 ± 0.02
5.648 ± 0.036
3.577 ± 0.023
3.484 ± 0.022
3.891 ± 0.029
6.559 ± 0.03
5.557 ± 0.049
6.38 ± 0.03
1.149 ± 0.015
3.844 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here