Apis mellifera associated microvirus 51

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q8U5I5|A0A3Q8U5I5_9VIRU DNA pilot protein OS=Apis mellifera associated microvirus 51 OX=2494782 PE=4 SV=1
MM1 pKa = 7.6SKK3 pKa = 10.56LDD5 pKa = 3.9EE6 pKa = 5.13KK7 pKa = 11.16GHH9 pKa = 5.99EE10 pKa = 4.3VLDD13 pKa = 4.25SKK15 pKa = 11.02PVEE18 pKa = 4.62APVKK22 pKa = 8.95WRR24 pKa = 11.84RR25 pKa = 11.84PPTIQEE31 pKa = 4.24MISNAIRR38 pKa = 11.84SGEE41 pKa = 3.88LARR44 pKa = 11.84FAEE47 pKa = 4.52QQGHH51 pKa = 4.92EE52 pKa = 4.42TPEE55 pKa = 3.89EE56 pKa = 4.04ADD58 pKa = 4.16DD59 pKa = 4.56FDD61 pKa = 5.66VGDD64 pKa = 5.17DD65 pKa = 4.04YY66 pKa = 11.99DD67 pKa = 3.86PSSPYY72 pKa = 10.12EE73 pKa = 3.62EE74 pKa = 5.14HH75 pKa = 7.18FDD77 pKa = 4.01HH78 pKa = 6.53IQNFEE83 pKa = 4.09ALRR86 pKa = 11.84HH87 pKa = 4.37QVKK90 pKa = 9.95EE91 pKa = 4.11NKK93 pKa = 8.9KK94 pKa = 9.28RR95 pKa = 11.84RR96 pKa = 11.84YY97 pKa = 8.38ATEE100 pKa = 3.73EE101 pKa = 4.11VPKK104 pKa = 10.59KK105 pKa = 9.92KK106 pKa = 10.28AKK108 pKa = 10.26KK109 pKa = 9.27EE110 pKa = 3.98PKK112 pKa = 9.79EE113 pKa = 4.22DD114 pKa = 3.69DD115 pKa = 3.96SEE117 pKa = 6.21AEE119 pKa = 4.11TT120 pKa = 4.48

Molecular weight: 13.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S8UU63|A0A3S8UU63_9VIRU Major capsid protein OS=Apis mellifera associated microvirus 51 OX=2494782 PE=3 SV=1
MM1 pKa = 7.19SRR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84SSRR8 pKa = 11.84QRR10 pKa = 11.84RR11 pKa = 11.84DD12 pKa = 2.94STHH15 pKa = 6.42IANQRR20 pKa = 11.84LPLSPLEE27 pKa = 3.83NLFRR31 pKa = 11.84VTKK34 pKa = 9.38PPLVVFEE41 pKa = 6.04DD42 pKa = 3.49RR43 pKa = 11.84RR44 pKa = 11.84QWHH47 pKa = 6.93PEE49 pKa = 3.42GKK51 pKa = 9.42YY52 pKa = 10.26APARR56 pKa = 11.84SFSSSRR62 pKa = 11.84HH63 pKa = 4.96RR64 pKa = 11.84LRR66 pKa = 11.84EE67 pKa = 3.62VPGISRR73 pKa = 11.84GNSRR77 pKa = 11.84NDD79 pKa = 2.97RR80 pKa = 11.84SRR82 pKa = 11.84RR83 pKa = 11.84SSGFRR88 pKa = 11.84LHH90 pKa = 7.52DD91 pKa = 4.86PGPLKK96 pKa = 10.52IGFGNPSRR104 pKa = 11.84VLVCVRR110 pKa = 11.84RR111 pKa = 11.84RR112 pKa = 11.84IRR114 pKa = 11.84KK115 pKa = 7.92EE116 pKa = 3.4VLFARR121 pKa = 11.84RR122 pKa = 11.84KK123 pKa = 7.09TGRR126 pKa = 11.84VGQRR130 pKa = 11.84RR131 pKa = 11.84PRR133 pKa = 11.84FNFYY137 pKa = 10.91SQISCRR143 pKa = 11.84RR144 pKa = 3.41

Molecular weight: 17.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1354

120

504

270.8

30.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.721 ± 1.06

1.108 ± 0.672

5.022 ± 0.655

6.352 ± 1.242

4.21 ± 0.227

8.124 ± 0.717

2.363 ± 0.346

4.727 ± 0.402

5.761 ± 1.088

7.533 ± 0.906

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.994 ± 0.207

4.21 ± 0.29

6.056 ± 0.643

5.022 ± 1.051

8.346 ± 2.049

7.164 ± 0.636

5.318 ± 0.941

5.022 ± 0.42

1.699 ± 0.218

3.25 ± 0.82
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski