Chryseobacterium sp. FH1
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3367 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A085B732|A0A085B732_9FLAO Amino acid racemase OS=Chryseobacterium sp. FH1 OX=1233951 GN=IO90_18840 PE=4 SV=1
MM1 pKa = 6.86 KK2 pKa = 9.75 TIIIGAIALLTMNFSFAQSNVDD24 pKa = 3.98 LSAQIGNLNTATVDD38 pKa = 3.06 QTGFMNFNALLQDD51 pKa = 3.98 GNRR54 pKa = 11.84 NDD56 pKa = 4.49 ADD58 pKa = 3.49 IDD60 pKa = 3.75 QVGWGNSNLALSQGNRR76 pKa = 11.84 NSIDD80 pKa = 3.13 VDD82 pKa = 2.98 QWGIGNSNTTSQYY95 pKa = 10.76 GNRR98 pKa = 11.84 NSSQTLQVGLFNDD111 pKa = 3.44 VDD113 pKa = 3.71 QVQIGRR119 pKa = 11.84 RR120 pKa = 11.84 NDD122 pKa = 3.04 ASATQFGMGNTIGQYY137 pKa = 9.68 QDD139 pKa = 2.72 GRR141 pKa = 11.84 RR142 pKa = 11.84 NSATAIQVGVDD153 pKa = 3.11 NTIWQDD159 pKa = 3.12 QYY161 pKa = 11.49 GRR163 pKa = 11.84 RR164 pKa = 11.84 NVAYY168 pKa = 9.7 AFQAGSDD175 pKa = 3.78 NYY177 pKa = 10.13 IHH179 pKa = 6.62 QLQDD183 pKa = 3.34 GNDD186 pKa = 3.34 NSATHH191 pKa = 6.21 LQFGDD196 pKa = 3.37 SNYY199 pKa = 10.58 ADD201 pKa = 3.24 SHH203 pKa = 6.82 QYY205 pKa = 11.44 GNDD208 pKa = 3.0 NTTAGLQVGNGNEE221 pKa = 4.16 LYY223 pKa = 10.31 QYY225 pKa = 11.04 QYY227 pKa = 11.84 GNGNTAMDD235 pKa = 3.68 IQMGDD240 pKa = 2.99 SNYY243 pKa = 10.2 TDD245 pKa = 3.37 VTQTGTSHH253 pKa = 6.9 LHH255 pKa = 5.87 MGMQAGNNNSLVVNQSNN272 pKa = 2.98
Molecular weight: 29.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.845
IPC2_protein 4.088
IPC_protein 4.113
Toseland 3.859
ProMoST 4.317
Dawson 4.139
Bjellqvist 4.291
Wikipedia 4.139
Rodwell 3.935
Grimsley 3.77
Solomon 4.139
Lehninger 4.101
Nozaki 4.253
DTASelect 4.622
Thurlkill 3.948
EMBOSS 4.151
Sillero 4.253
Patrickios 2.003
IPC_peptide 4.126
IPC2_peptide 4.215
IPC2.peptide.svr19 4.109
Protein with the highest isoelectric point:
>tr|A0A085BA57|A0A085BA57_9FLAO Uncharacterized protein OS=Chryseobacterium sp. FH1 OX=1233951 GN=IO90_08585 PE=4 SV=1
MM1 pKa = 7.21 TPGRR5 pKa = 11.84 EE6 pKa = 4.16 KK7 pKa = 10.59 PPHH10 pKa = 6.29 LLALAPLQRR19 pKa = 11.84 RR20 pKa = 11.84 GMGFFCIGYY29 pKa = 9.41 ILQKK33 pKa = 8.73 PSKK36 pKa = 8.46 YY37 pKa = 10.71 AEE39 pKa = 4.43 DD40 pKa = 3.98 RR41 pKa = 11.84 STASGVANRR50 pKa = 11.84 YY51 pKa = 7.75 IRR53 pKa = 11.84 KK54 pKa = 9.15 ILPPTLKK61 pKa = 10.76 GGIFPVGLKK70 pKa = 10.18 LL71 pKa = 4.29
Molecular weight: 7.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.311
IPC2_protein 9.911
IPC_protein 10.526
Toseland 10.73
ProMoST 10.35
Dawson 10.833
Bjellqvist 10.496
Wikipedia 11.008
Rodwell 11.228
Grimsley 10.877
Solomon 10.906
Lehninger 10.877
Nozaki 10.701
DTASelect 10.496
Thurlkill 10.716
EMBOSS 11.111
Sillero 10.745
Patrickios 10.994
IPC_peptide 10.906
IPC2_peptide 9.385
IPC2.peptide.svr19 8.442
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3367
0
3367
1084421
54
2373
322.1
36.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.944 ± 0.04
0.677 ± 0.011
5.575 ± 0.031
6.78 ± 0.049
5.584 ± 0.036
6.176 ± 0.046
1.58 ± 0.02
8.053 ± 0.043
8.443 ± 0.046
9.136 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.217 ± 0.023
6.538 ± 0.045
3.3 ± 0.022
3.502 ± 0.024
3.226 ± 0.023
6.755 ± 0.036
5.439 ± 0.041
5.946 ± 0.031
1.026 ± 0.014
4.103 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here