Streptomyces sp. WM6386

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8143 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F5VQI0|A0A0F5VQI0_9ACTN Amidotransferase OS=Streptomyces sp. WM6386 OX=1415558 GN=TN53_30130 PE=4 SV=1
MM1 pKa = 7.5SVQQEE6 pKa = 3.94AVADD10 pKa = 4.37GEE12 pKa = 4.2ALEE15 pKa = 4.22VWIDD19 pKa = 3.41QDD21 pKa = 3.99LCTGDD26 pKa = 5.01GICAQYY32 pKa = 10.9APEE35 pKa = 4.23VFEE38 pKa = 5.9LDD40 pKa = 3.05IDD42 pKa = 3.65GLAYY46 pKa = 10.67VKK48 pKa = 10.82GAGDD52 pKa = 3.89EE53 pKa = 4.02LLQAKK58 pKa = 9.39GATTPVPLPLLTDD71 pKa = 3.6VVDD74 pKa = 4.12SAKK77 pKa = 10.15EE78 pKa = 3.93CPGEE82 pKa = 4.38CIHH85 pKa = 6.23VRR87 pKa = 11.84RR88 pKa = 11.84VSDD91 pKa = 3.5LVEE94 pKa = 4.31VYY96 pKa = 10.72GPEE99 pKa = 3.79ADD101 pKa = 3.38

Molecular weight: 10.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F5W5F8|A0A0F5W5F8_9ACTN Lipoprotein OS=Streptomyces sp. WM6386 OX=1415558 GN=TN53_04150 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.51GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8140

3

8143

2521063

29

3628

309.6

33.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.113 ± 0.034

0.768 ± 0.007

6.05 ± 0.02

5.806 ± 0.03

2.82 ± 0.018

9.369 ± 0.025

2.261 ± 0.012

3.316 ± 0.018

2.388 ± 0.02

10.322 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.765 ± 0.012

1.876 ± 0.015

5.822 ± 0.023

2.832 ± 0.018

7.683 ± 0.035

5.173 ± 0.022

6.262 ± 0.027

8.579 ± 0.026

1.578 ± 0.013

2.217 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski