Amino acid dipepetide frequency for Streptomyces sp. WM6386

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.725AlaAla: 19.725 ± 0.14
1.006AlaCys: 1.006 ± 0.021
8.073AlaAsp: 8.073 ± 0.059
8.564AlaGlu: 8.564 ± 0.078
3.514AlaPhe: 3.514 ± 0.045
12.238AlaGly: 12.238 ± 0.08
2.777AlaHis: 2.777 ± 0.035
3.658AlaIle: 3.658 ± 0.042
3.089AlaLys: 3.089 ± 0.047
14.208AlaLeu: 14.208 ± 0.096
2.464AlaMet: 2.464 ± 0.037
2.035AlaAsn: 2.035 ± 0.032
6.413AlaPro: 6.413 ± 0.061
3.899AlaGln: 3.899 ± 0.041
9.605AlaArg: 9.605 ± 0.072
6.007AlaSer: 6.007 ± 0.053
7.043AlaThr: 7.043 ± 0.048
12.041AlaVal: 12.041 ± 0.09
1.892AlaTrp: 1.892 ± 0.026
2.877AlaTyr: 2.877 ± 0.039
0.0AlaXaa: 0.0 ± 0.0
Cys
1.063CysAla: 1.063 ± 0.023
0.094CysCys: 0.094 ± 0.006
0.46CysAsp: 0.46 ± 0.014
0.414CysGlu: 0.414 ± 0.015
0.217CysPhe: 0.217 ± 0.009
0.958CysGly: 0.958 ± 0.022
0.179CysHis: 0.179 ± 0.008
0.165CysIle: 0.165 ± 0.008
0.115CysLys: 0.115 ± 0.007
0.735CysLeu: 0.735 ± 0.017
0.126CysMet: 0.126 ± 0.007
0.127CysAsn: 0.127 ± 0.007
0.453CysPro: 0.453 ± 0.016
0.158CysGln: 0.158 ± 0.009
0.557CysArg: 0.557 ± 0.016
0.411CysSer: 0.411 ± 0.012
0.523CysThr: 0.523 ± 0.015
0.656CysVal: 0.656 ± 0.018
0.126CysTrp: 0.126 ± 0.007
0.148CysTyr: 0.148 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
7.416AspAla: 7.416 ± 0.059
0.422AspCys: 0.422 ± 0.014
3.695AspAsp: 3.695 ± 0.045
3.964AspGlu: 3.964 ± 0.052
1.762AspPhe: 1.762 ± 0.028
6.43AspGly: 6.43 ± 0.062
1.414AspHis: 1.414 ± 0.022
2.06AspIle: 2.06 ± 0.029
1.369AspLys: 1.369 ± 0.027
6.126AspLeu: 6.126 ± 0.054
0.879AspMet: 0.879 ± 0.018
1.051AspAsn: 1.051 ± 0.02
4.405AspPro: 4.405 ± 0.048
1.555AspGln: 1.555 ± 0.026
4.681AspArg: 4.681 ± 0.05
2.722AspSer: 2.722 ± 0.038
3.343AspThr: 3.343 ± 0.033
4.946AspVal: 4.946 ± 0.047
1.105AspTrp: 1.105 ± 0.022
1.153AspTyr: 1.153 ± 0.024
0.0AspXaa: 0.0 ± 0.0
Glu
7.314GluAla: 7.314 ± 0.077
0.354GluCys: 0.354 ± 0.012
2.921GluAsp: 2.921 ± 0.039
3.666GluGlu: 3.666 ± 0.047
1.576GluPhe: 1.576 ± 0.025
4.528GluGly: 4.528 ± 0.051
1.498GluHis: 1.498 ± 0.026
2.365GluIle: 2.365 ± 0.04
1.619GluLys: 1.619 ± 0.028
7.001GluLeu: 7.001 ± 0.066
0.899GluMet: 0.899 ± 0.022
1.09GluAsn: 1.09 ± 0.023
3.389GluPro: 3.389 ± 0.044
2.278GluGln: 2.278 ± 0.033
5.297GluArg: 5.297 ± 0.061
2.681GluSer: 2.681 ± 0.031
2.992GluThr: 2.992 ± 0.038
4.582GluVal: 4.582 ± 0.045
0.812GluTrp: 0.812 ± 0.017
1.201GluTyr: 1.201 ± 0.023
0.0GluXaa: 0.0 ± 0.0
Phe
3.656PheAla: 3.656 ± 0.041
0.265PheCys: 0.265 ± 0.01
1.969PheAsp: 1.969 ± 0.034
1.507PheGlu: 1.507 ± 0.027
0.918PhePhe: 0.918 ± 0.024
3.128PheGly: 3.128 ± 0.044
0.617PheHis: 0.617 ± 0.016
0.762PheIle: 0.762 ± 0.02
0.597PheLys: 0.597 ± 0.017
2.665PheLeu: 2.665 ± 0.038
0.432PheMet: 0.432 ± 0.014
0.621PheAsn: 0.621 ± 0.017
1.416PhePro: 1.416 ± 0.027
0.72PheGln: 0.72 ± 0.017
1.868PheArg: 1.868 ± 0.03
1.522PheSer: 1.522 ± 0.027
2.09PheThr: 2.09 ± 0.034
2.347PheVal: 2.347 ± 0.035
0.464PheTrp: 0.464 ± 0.014
0.635PheTyr: 0.635 ± 0.016
0.0PheXaa: 0.0 ± 0.0
Gly
10.69GlyAla: 10.69 ± 0.073
0.816GlyCys: 0.816 ± 0.018
5.191GlyAsp: 5.191 ± 0.052
5.137GlyGlu: 5.137 ± 0.057
2.998GlyPhe: 2.998 ± 0.039
8.588GlyGly: 8.588 ± 0.078
2.22GlyHis: 2.22 ± 0.034
3.62GlyIle: 3.62 ± 0.035
2.633GlyLys: 2.633 ± 0.043
9.437GlyLeu: 9.437 ± 0.066
2.017GlyMet: 2.017 ± 0.028
1.813GlyAsn: 1.813 ± 0.035
4.815GlyPro: 4.815 ± 0.055
2.693GlyGln: 2.693 ± 0.033
7.369GlyArg: 7.369 ± 0.059
5.424GlySer: 5.424 ± 0.057
6.465GlyThr: 6.465 ± 0.059
7.623GlyVal: 7.623 ± 0.059
1.764GlyTrp: 1.764 ± 0.026
2.381GlyTyr: 2.381 ± 0.034
0.0GlyXaa: 0.0 ± 0.0
His
2.598HisAla: 2.598 ± 0.038
0.218HisCys: 0.218 ± 0.01
1.345HisAsp: 1.345 ± 0.022
1.263HisGlu: 1.263 ± 0.022
0.662HisPhe: 0.662 ± 0.017
2.345HisGly: 2.345 ± 0.036
0.686HisHis: 0.686 ± 0.018
0.712HisIle: 0.712 ± 0.018
0.366HisLys: 0.366 ± 0.012
2.376HisLeu: 2.376 ± 0.033
0.338HisMet: 0.338 ± 0.01
0.386HisAsn: 0.386 ± 0.014
1.716HisPro: 1.716 ± 0.027
0.637HisGln: 0.637 ± 0.017
2.001HisArg: 2.001 ± 0.028
1.018HisSer: 1.018 ± 0.021
1.366HisThr: 1.366 ± 0.024
1.693HisVal: 1.693 ± 0.025
0.385HisTrp: 0.385 ± 0.014
0.501HisTyr: 0.501 ± 0.016
0.0HisXaa: 0.0 ± 0.0
Ile
5.012IleAla: 5.012 ± 0.052
0.277IleCys: 0.277 ± 0.012
2.362IleAsp: 2.362 ± 0.04
2.063IleGlu: 2.063 ± 0.031
0.743IlePhe: 0.743 ± 0.019
3.692IleGly: 3.692 ± 0.037
0.667IleHis: 0.667 ± 0.018
0.931IleIle: 0.931 ± 0.021
0.801IleLys: 0.801 ± 0.02
2.573IleLeu: 2.573 ± 0.034
0.465IleMet: 0.465 ± 0.015
0.733IleAsn: 0.733 ± 0.019
1.852IlePro: 1.852 ± 0.03
0.77IleGln: 0.77 ± 0.019
2.259IleArg: 2.259 ± 0.034
1.754IleSer: 1.754 ± 0.03
2.352IleThr: 2.352 ± 0.032
2.867IleVal: 2.867 ± 0.033
0.41IleTrp: 0.41 ± 0.015
0.576IleTyr: 0.576 ± 0.016
0.0IleXaa: 0.0 ± 0.0
Lys
3.263LysAla: 3.263 ± 0.041
0.131LysCys: 0.131 ± 0.009
1.478LysAsp: 1.478 ± 0.025
1.346LysGlu: 1.346 ± 0.025
0.516LysPhe: 0.516 ± 0.015
2.06LysGly: 2.06 ± 0.036
0.458LysHis: 0.458 ± 0.013
0.921LysIle: 0.921 ± 0.019
1.018LysLys: 1.018 ± 0.027
2.244LysLeu: 2.244 ± 0.031
0.399LysMet: 0.399 ± 0.011
0.601LysAsn: 0.601 ± 0.018
1.419LysPro: 1.419 ± 0.027
0.77LysGln: 0.77 ± 0.021
1.485LysArg: 1.485 ± 0.028
1.339LysSer: 1.339 ± 0.028
1.407LysThr: 1.407 ± 0.029
2.171LysVal: 2.171 ± 0.036
0.315LysTrp: 0.315 ± 0.011
0.538LysTyr: 0.538 ± 0.014
0.0LysXaa: 0.0 ± 0.0
Leu
14.683LeuAla: 14.683 ± 0.099
0.831LeuCys: 0.831 ± 0.019
6.733LeuAsp: 6.733 ± 0.056
4.819LeuGlu: 4.819 ± 0.057
2.651LeuPhe: 2.651 ± 0.04
9.312LeuGly: 9.312 ± 0.07
2.282LeuHis: 2.282 ± 0.033
3.408LeuIle: 3.408 ± 0.043
2.311LeuLys: 2.311 ± 0.034
11.274LeuLeu: 11.274 ± 0.099
1.687LeuMet: 1.687 ± 0.029
1.791LeuAsn: 1.791 ± 0.025
6.262LeuPro: 6.262 ± 0.063
2.242LeuGln: 2.242 ± 0.031
8.389LeuArg: 8.389 ± 0.066
5.326LeuSer: 5.326 ± 0.049
7.027LeuThr: 7.027 ± 0.048
8.904LeuVal: 8.904 ± 0.062
1.33LeuTrp: 1.33 ± 0.022
1.957LeuTyr: 1.957 ± 0.029
0.0LeuXaa: 0.0 ± 0.0
Met
2.242MetAla: 2.242 ± 0.033
0.139MetCys: 0.139 ± 0.007
0.913MetAsp: 0.913 ± 0.02
0.777MetGlu: 0.777 ± 0.019
0.473MetPhe: 0.473 ± 0.013
1.349MetGly: 1.349 ± 0.027
0.347MetHis: 0.347 ± 0.013
0.658MetIle: 0.658 ± 0.018
0.444MetLys: 0.444 ± 0.015
1.694MetLeu: 1.694 ± 0.031
0.302MetMet: 0.302 ± 0.012
0.442MetAsn: 0.442 ± 0.013
1.104MetPro: 1.104 ± 0.024
0.446MetGln: 0.446 ± 0.013
1.425MetArg: 1.425 ± 0.025
1.341MetSer: 1.341 ± 0.019
1.639MetThr: 1.639 ± 0.027
1.331MetVal: 1.331 ± 0.022
0.23MetTrp: 0.23 ± 0.008
0.351MetTyr: 0.351 ± 0.012
0.0MetXaa: 0.0 ± 0.0
Asn
2.288AsnAla: 2.288 ± 0.033
0.165AsnCys: 0.165 ± 0.008
1.02AsnAsp: 1.02 ± 0.022
0.878AsnGlu: 0.878 ± 0.018
0.541AsnPhe: 0.541 ± 0.015
1.997AsnGly: 1.997 ± 0.035
0.401AsnHis: 0.401 ± 0.013
0.705AsnIle: 0.705 ± 0.017
0.468AsnLys: 0.468 ± 0.015
1.733AsnLeu: 1.733 ± 0.026
0.316AsnMet: 0.316 ± 0.012
0.514AsnAsn: 0.514 ± 0.016
1.393AsnPro: 1.393 ± 0.024
0.548AsnGln: 0.548 ± 0.016
1.27AsnArg: 1.27 ± 0.023
1.06AsnSer: 1.06 ± 0.024
1.217AsnThr: 1.217 ± 0.025
1.453AsnVal: 1.453 ± 0.024
0.326AsnTrp: 0.326 ± 0.012
0.467AsnTyr: 0.467 ± 0.014
0.0AsnXaa: 0.0 ± 0.0
Pro
7.684ProAla: 7.684 ± 0.062
0.316ProCys: 0.316 ± 0.011
4.382ProAsp: 4.382 ± 0.043
4.301ProGlu: 4.301 ± 0.046
1.527ProPhe: 1.527 ± 0.028
6.113ProGly: 6.113 ± 0.055
1.361ProHis: 1.361 ± 0.023
1.341ProIle: 1.341 ± 0.023
1.287ProLys: 1.287 ± 0.023
5.248ProLeu: 5.248 ± 0.047
0.986ProMet: 0.986 ± 0.021
0.931ProAsn: 0.931 ± 0.021
3.192ProPro: 3.192 ± 0.045
1.789ProGln: 1.789 ± 0.027
3.642ProArg: 3.642 ± 0.045
3.155ProSer: 3.155 ± 0.042
3.246ProThr: 3.246 ± 0.037
5.331ProVal: 5.331 ± 0.044
0.904ProTrp: 0.904 ± 0.018
1.479ProTyr: 1.479 ± 0.025
0.0ProXaa: 0.0 ± 0.0
Gln
3.667GlnAla: 3.667 ± 0.046
0.182GlnCys: 0.182 ± 0.009
1.491GlnAsp: 1.491 ± 0.025
1.566GlnGlu: 1.566 ± 0.027
0.714GlnPhe: 0.714 ± 0.018
2.428GlnGly: 2.428 ± 0.034
0.673GlnHis: 0.673 ± 0.017
1.145GlnIle: 1.145 ± 0.023
0.686GlnLys: 0.686 ± 0.017
3.145GlnLeu: 3.145 ± 0.036
0.513GlnMet: 0.513 ± 0.014
0.552GlnAsn: 0.552 ± 0.017
1.673GlnPro: 1.673 ± 0.029
1.297GlnGln: 1.297 ± 0.037
2.257GlnArg: 2.257 ± 0.028
1.35GlnSer: 1.35 ± 0.025
1.416GlnThr: 1.416 ± 0.027
2.396GlnVal: 2.396 ± 0.033
0.501GlnTrp: 0.501 ± 0.013
0.67GlnTyr: 0.67 ± 0.016
0.0GlnXaa: 0.0 ± 0.0
Arg
9.256ArgAla: 9.256 ± 0.068
0.544ArgCys: 0.544 ± 0.016
4.089ArgAsp: 4.089 ± 0.039
4.746ArgGlu: 4.746 ± 0.05
2.289ArgPhe: 2.289 ± 0.03
5.576ArgGly: 5.576 ± 0.056
2.015ArgHis: 2.015 ± 0.03
3.245ArgIle: 3.245 ± 0.044
1.737ArgLys: 1.737 ± 0.031
8.498ArgLeu: 8.498 ± 0.068
1.66ArgMet: 1.66 ± 0.026
1.34ArgAsn: 1.34 ± 0.026
4.522ArgPro: 4.522 ± 0.047
2.236ArgGln: 2.236 ± 0.03
7.135ArgArg: 7.135 ± 0.065
3.872ArgSer: 3.872 ± 0.043
5.24ArgThr: 5.24 ± 0.045
5.728ArgVal: 5.728 ± 0.051
1.331ArgTrp: 1.331 ± 0.024
1.775ArgTyr: 1.775 ± 0.027
0.0ArgXaa: 0.0 ± 0.0
Ser
6.683SerAla: 6.683 ± 0.05
0.407SerCys: 0.407 ± 0.012
2.902SerAsp: 2.902 ± 0.039
2.511SerGlu: 2.511 ± 0.034
1.593SerPhe: 1.593 ± 0.026
6.028SerGly: 6.028 ± 0.053
1.023SerHis: 1.023 ± 0.021
1.503SerIle: 1.503 ± 0.026
1.16SerLys: 1.16 ± 0.026
4.943SerLeu: 4.943 ± 0.045
1.117SerMet: 1.117 ± 0.021
0.954SerAsn: 0.954 ± 0.022
3.085SerPro: 3.085 ± 0.038
1.335SerGln: 1.335 ± 0.025
3.567SerArg: 3.567 ± 0.04
3.012SerSer: 3.012 ± 0.044
3.178SerThr: 3.178 ± 0.043
4.417SerVal: 4.417 ± 0.044
0.938SerTrp: 0.938 ± 0.018
1.375SerTyr: 1.375 ± 0.026
0.0SerXaa: 0.0 ± 0.0
Thr
8.725ThrAla: 8.725 ± 0.071
0.422ThrCys: 0.422 ± 0.015
3.833ThrAsp: 3.833 ± 0.043
3.466ThrGlu: 3.466 ± 0.045
1.695ThrPhe: 1.695 ± 0.03
6.7ThrGly: 6.7 ± 0.057
1.23ThrHis: 1.23 ± 0.022
1.831ThrIle: 1.831 ± 0.028
1.374ThrLys: 1.374 ± 0.029
5.851ThrLeu: 5.851 ± 0.046
0.996ThrMet: 0.996 ± 0.021
1.084ThrAsn: 1.084 ± 0.024
4.094ThrPro: 4.094 ± 0.047
1.439ThrGln: 1.439 ± 0.025
3.824ThrArg: 3.824 ± 0.037
3.322ThrSer: 3.322 ± 0.04
4.003ThrThr: 4.003 ± 0.05
6.257ThrVal: 6.257 ± 0.052
0.983ThrTrp: 0.983 ± 0.023
1.494ThrTyr: 1.494 ± 0.025
0.0ThrXaa: 0.0 ± 0.0
Val
10.591ValAla: 10.591 ± 0.076
0.774ValCys: 0.774 ± 0.021
5.055ValAsp: 5.055 ± 0.052
4.909ValGlu: 4.909 ± 0.05
2.552ValPhe: 2.552 ± 0.037
6.635ValGly: 6.635 ± 0.06
1.989ValHis: 1.989 ± 0.03
2.952ValIle: 2.952 ± 0.038
1.913ValLys: 1.913 ± 0.034
9.546ValLeu: 9.546 ± 0.079
1.46ValMet: 1.46 ± 0.025
1.749ValAsn: 1.749 ± 0.029
5.169ValPro: 5.169 ± 0.049
2.214ValGln: 2.214 ± 0.03
7.092ValArg: 7.092 ± 0.061
4.441ValSer: 4.441 ± 0.046
5.679ValThr: 5.679 ± 0.044
8.193ValVal: 8.193 ± 0.066
1.205ValTrp: 1.205 ± 0.026
1.671ValTyr: 1.671 ± 0.028
0.0ValXaa: 0.0 ± 0.0
Trp
1.718TrpAla: 1.718 ± 0.028
0.139TrpCys: 0.139 ± 0.007
0.904TrpAsp: 0.904 ± 0.021
0.774TrpGlu: 0.774 ± 0.019
0.543TrpPhe: 0.543 ± 0.017
1.117TrpGly: 1.117 ± 0.022
0.376TrpHis: 0.376 ± 0.012
0.615TrpIle: 0.615 ± 0.015
0.412TrpLys: 0.412 ± 0.013
1.811TrpLeu: 1.811 ± 0.034
0.314TrpMet: 0.314 ± 0.011
0.476TrpAsn: 0.476 ± 0.015
0.793TrpPro: 0.793 ± 0.02
0.654TrpGln: 0.654 ± 0.016
1.298TrpArg: 1.298 ± 0.026
0.961TrpSer: 0.961 ± 0.02
1.102TrpThr: 1.102 ± 0.023
1.013TrpVal: 1.013 ± 0.021
0.367TrpTrp: 0.367 ± 0.013
0.392TrpTyr: 0.392 ± 0.012
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.857TyrAla: 2.857 ± 0.037
0.181TyrCys: 0.181 ± 0.009
1.683TyrAsp: 1.683 ± 0.029
1.393TyrGlu: 1.393 ± 0.022
0.718TyrPhe: 0.718 ± 0.018
2.473TyrGly: 2.473 ± 0.033
0.395TyrHis: 0.395 ± 0.014
0.563TyrIle: 0.563 ± 0.015
0.48TyrLys: 0.48 ± 0.016
2.112TyrLeu: 2.112 ± 0.03
0.276TyrMet: 0.276 ± 0.011
0.47TyrAsn: 0.47 ± 0.016
1.052TyrPro: 1.052 ± 0.025
0.641TyrGln: 0.641 ± 0.018
1.809TyrArg: 1.809 ± 0.032
1.013TyrSer: 1.013 ± 0.022
1.292TyrThr: 1.292 ± 0.026
1.842TyrVal: 1.842 ± 0.029
0.391TyrTrp: 0.391 ± 0.014
0.528TyrTyr: 0.528 ± 0.017
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.001XaaXaa: 0.001 ± 0.001
Statistics based on 8143 proteins (2521063 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski