South African cassava mosaic virus
Average proteome isoelectric point is 8.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9IN43|Q9IN43_9GEMI Transcriptional activator protein OS=South African cassava mosaic virus OX=63723 GN=AC2 PE=3 SV=1
MM1 pKa = 7.35 PRR3 pKa = 11.84 AGRR6 pKa = 11.84 FSIKK10 pKa = 10.18 AKK12 pKa = 10.62 NYY14 pKa = 8.28 FLTYY18 pKa = 9.38 PKK20 pKa = 9.76 CTLSKK25 pKa = 10.51 EE26 pKa = 4.07 AALDD30 pKa = 3.53 QLRR33 pKa = 11.84 QLQTPTNKK41 pKa = 10.03 LFIKK45 pKa = 10.0 ICRR48 pKa = 11.84 EE49 pKa = 3.52 LHH51 pKa = 6.26 EE52 pKa = 4.93 NGEE55 pKa = 4.34 PHH57 pKa = 6.73 LHH59 pKa = 6.75 ALIQFEE65 pKa = 4.49 GKK67 pKa = 10.14 YY68 pKa = 10.4 NCTNQRR74 pKa = 11.84 FFDD77 pKa = 5.08 LISPSRR83 pKa = 11.84 STHH86 pKa = 4.86 FHH88 pKa = 6.77 PNIQGAKK95 pKa = 9.25 SSSDD99 pKa = 3.17 VKK101 pKa = 11.17 SYY103 pKa = 11.2 LDD105 pKa = 3.72 KK106 pKa = 11.6 DD107 pKa = 3.4 GDD109 pKa = 4.14 TIQWGEE115 pKa = 3.74 FQIDD119 pKa = 3.16 GRR121 pKa = 11.84 SARR124 pKa = 11.84 GGQQSANDD132 pKa = 4.12 AYY134 pKa = 10.91 AKK136 pKa = 10.52 ALNAASKK143 pKa = 9.7 TEE145 pKa = 3.75 ALNVIRR151 pKa = 11.84 EE152 pKa = 4.21 LAPKK156 pKa = 10.52 DD157 pKa = 3.64 FVLQFHH163 pKa = 6.68 NLNSNLDD170 pKa = 4.08 RR171 pKa = 11.84 IFQEE175 pKa = 3.93 PPIPYY180 pKa = 9.4 ISPFLSSSFTHH191 pKa = 5.96 VPEE194 pKa = 4.46 EE195 pKa = 4.19 LEE197 pKa = 4.11 DD198 pKa = 3.63 WVSEE202 pKa = 3.99 NVMGFAARR210 pKa = 11.84 PWRR213 pKa = 11.84 PSSIVIEE220 pKa = 4.46 GDD222 pKa = 3.11 SRR224 pKa = 11.84 TGKK227 pKa = 8.52 TMWARR232 pKa = 11.84 SLGPHH237 pKa = 6.71 NYY239 pKa = 10.18 LCGHH243 pKa = 7.38 LDD245 pKa = 4.18 LSPKK249 pKa = 10.15 VYY251 pKa = 11.07 SNDD254 pKa = 2.25 AWYY257 pKa = 10.92 NVIDD261 pKa = 5.2 DD262 pKa = 4.41 VDD264 pKa = 3.61 PHH266 pKa = 5.85 YY267 pKa = 11.0 LKK269 pKa = 10.7 HH270 pKa = 6.2 FKK272 pKa = 10.7 EE273 pKa = 4.46 FMGAQRR279 pKa = 11.84 DD280 pKa = 3.81 WQSNTKK286 pKa = 9.26 YY287 pKa = 10.63 GKK289 pKa = 9.49 PIQIKK294 pKa = 10.42 GGIPTIFLCNPGPTSSYY311 pKa = 11.31 KK312 pKa = 10.67 EE313 pKa = 3.89 FLDD316 pKa = 3.75 EE317 pKa = 5.39 EE318 pKa = 4.82 KK319 pKa = 11.0 NQSLKK324 pKa = 10.9 AWALKK329 pKa = 10.07 NATFITLHH337 pKa = 6.27 EE338 pKa = 4.47 PLFSSAHH345 pKa = 4.89 QSPTPHH351 pKa = 7.15 RR352 pKa = 11.84 EE353 pKa = 3.83 DD354 pKa = 3.16
Molecular weight: 40.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.64
IPC2_protein 6.664
IPC_protein 6.825
Toseland 6.942
ProMoST 7.073
Dawson 7.337
Bjellqvist 7.234
Wikipedia 7.307
Rodwell 7.322
Grimsley 7.088
Solomon 7.38
Lehninger 7.395
Nozaki 7.571
DTASelect 7.614
Thurlkill 7.658
EMBOSS 7.688
Sillero 7.732
Patrickios 4.355
IPC_peptide 7.38
IPC2_peptide 7.146
IPC2.peptide.svr19 7.071
Protein with the highest isoelectric point:
>tr|Q9IN46|Q9IN46_9GEMI Protein V2 OS=South African cassava mosaic virus OX=63723 GN=AV2 PE=3 SV=1
MM1 pKa = 7.71 SKK3 pKa = 10.35 RR4 pKa = 11.84 PADD7 pKa = 3.94 IIISTPVSKK16 pKa = 10.56 VRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 LNFDD24 pKa = 3.38 SPFSSRR30 pKa = 11.84 AAVPIVQGTNKK41 pKa = 9.26 RR42 pKa = 11.84 RR43 pKa = 11.84 SWTFRR48 pKa = 11.84 PMYY51 pKa = 9.51 RR52 pKa = 11.84 KK53 pKa = 9.28 PRR55 pKa = 11.84 MYY57 pKa = 11.01 RR58 pKa = 11.84 MFKK61 pKa = 10.72 SPDD64 pKa = 3.23 VPRR67 pKa = 11.84 GCEE70 pKa = 4.34 GPCKK74 pKa = 9.32 VQSYY78 pKa = 6.99 EE79 pKa = 3.67 QRR81 pKa = 11.84 DD82 pKa = 4.06 DD83 pKa = 3.74 VKK85 pKa = 9.69 HH86 pKa = 5.74 TGSVRR91 pKa = 11.84 CVSDD95 pKa = 3.11 VTRR98 pKa = 11.84 GSGITHH104 pKa = 6.35 RR105 pKa = 11.84 VGKK108 pKa = 9.68 RR109 pKa = 11.84 FCIKK113 pKa = 10.26 SIYY116 pKa = 9.86 VLGKK120 pKa = 9.13 IWMDD124 pKa = 3.49 EE125 pKa = 4.09 NIKK128 pKa = 10.06 KK129 pKa = 9.97 QNHH132 pKa = 4.95 TNQVMFFLVRR142 pKa = 11.84 DD143 pKa = 3.63 RR144 pKa = 11.84 RR145 pKa = 11.84 PYY147 pKa = 10.91 GNSPMDD153 pKa = 3.85 FGQVFNMFDD162 pKa = 3.99 NEE164 pKa = 4.11 PSTATVKK171 pKa = 10.81 NDD173 pKa = 2.71 LRR175 pKa = 11.84 DD176 pKa = 3.32 RR177 pKa = 11.84 YY178 pKa = 9.7 RR179 pKa = 11.84 VMRR182 pKa = 11.84 KK183 pKa = 7.56 FHH185 pKa = 5.89 ATVVGGPSGMKK196 pKa = 9.32 EE197 pKa = 3.6 QALVRR202 pKa = 11.84 RR203 pKa = 11.84 FFRR206 pKa = 11.84 INNHH210 pKa = 4.14 VVYY213 pKa = 10.43 NHH215 pKa = 5.84 QEE217 pKa = 3.45 AAKK220 pKa = 10.4 YY221 pKa = 9.25 EE222 pKa = 4.13 NHH224 pKa = 6.46 TEE226 pKa = 3.99 NALLLYY232 pKa = 7.29 MACTHH237 pKa = 7.07 ASNPVYY243 pKa = 9.86 ATLKK247 pKa = 9.47 IRR249 pKa = 11.84 IYY251 pKa = 10.65 FYY253 pKa = 11.26 DD254 pKa = 3.34 AVTNN258 pKa = 4.16
Molecular weight: 30.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.309
IPC2_protein 9.648
IPC_protein 10.131
Toseland 10.335
ProMoST 10.028
Dawson 10.511
Bjellqvist 10.204
Wikipedia 10.701
Rodwell 10.818
Grimsley 10.57
Solomon 10.555
Lehninger 10.511
Nozaki 10.335
DTASelect 10.189
Thurlkill 10.365
EMBOSS 10.73
Sillero 10.423
Patrickios 10.452
IPC_peptide 10.555
IPC2_peptide 9.136
IPC2.peptide.svr19 8.598
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
1660
98
354
207.5
23.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.06 ± 0.697
1.807 ± 0.33
5.301 ± 0.416
4.217 ± 0.427
4.458 ± 0.389
5.181 ± 0.704
3.735 ± 0.862
5.602 ± 0.589
5.663 ± 0.399
7.59 ± 0.769
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.289 ± 0.494
5.361 ± 0.479
6.325 ± 0.434
4.458 ± 0.573
7.289 ± 0.83
8.434 ± 0.983
6.265 ± 0.713
5.783 ± 1.039
1.205 ± 0.187
3.976 ± 0.641
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here