Hanstruepera neustonica

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Hanstruepera

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2739 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K1DWZ9|A0A2K1DWZ9_9FLAO Uncharacterized protein OS=Hanstruepera neustonica OX=1445657 GN=C1T31_10450 PE=4 SV=1
MM1 pKa = 7.34RR2 pKa = 11.84QIGLLLFVFFTGLLQAQISFEE23 pKa = 4.19DD24 pKa = 3.6QASDD28 pKa = 4.13LGVQLSCGTTYY39 pKa = 11.02LGNGVSFYY47 pKa = 11.4DD48 pKa = 4.25FDD50 pKa = 4.65NDD52 pKa = 3.36GWDD55 pKa = 3.89DD56 pKa = 3.67LTLNTEE62 pKa = 4.68FGQEE66 pKa = 3.19IRR68 pKa = 11.84FFKK71 pKa = 9.2NTNGTFAEE79 pKa = 4.31IFPTIPYY86 pKa = 9.77FDD88 pKa = 4.24YY89 pKa = 9.74QTKK92 pKa = 8.93QVNWVDD98 pKa = 4.07FDD100 pKa = 4.14NDD102 pKa = 3.2GDD104 pKa = 3.89KK105 pKa = 11.47DD106 pKa = 4.15LFVTSDD112 pKa = 3.54YY113 pKa = 11.27EE114 pKa = 4.52GNRR117 pKa = 11.84LFEE120 pKa = 4.54NTGSMTFVDD129 pKa = 4.16ISVSSGIDD137 pKa = 3.14TTNEE141 pKa = 3.44FSYY144 pKa = 9.09GTSWGDD150 pKa = 3.53YY151 pKa = 10.93NNDD154 pKa = 3.11GYY156 pKa = 11.84LDD158 pKa = 3.45IFVSNRR164 pKa = 11.84TALHH168 pKa = 5.77NNILYY173 pKa = 10.46RR174 pKa = 11.84NNGDD178 pKa = 3.49GTFTDD183 pKa = 3.51VTLAAGLSGSGSGSFCSAFIDD204 pKa = 3.69INNDD208 pKa = 2.75GFQDD212 pKa = 3.66IYY214 pKa = 10.8ISNDD218 pKa = 2.87RR219 pKa = 11.84PVNPNILYY227 pKa = 10.46KK228 pKa = 11.13NNGDD232 pKa = 3.67GTFTDD237 pKa = 4.23ISASSGSDD245 pKa = 3.2VTIDD249 pKa = 3.24AMTVTVADD257 pKa = 4.54FNSDD261 pKa = 2.89GWFDD265 pKa = 3.4IYY267 pKa = 10.01ITNGPIGNVLLKK279 pKa = 11.16NNGNEE284 pKa = 4.15TFTDD288 pKa = 3.34IAVSSGTVFNSISWGASFFDD308 pKa = 4.87AEE310 pKa = 4.15NDD312 pKa = 3.37MDD314 pKa = 4.02QDD316 pKa = 4.46LYY318 pKa = 11.83VCGEE322 pKa = 4.17FDD324 pKa = 4.24ANQTNFLLAAFYY336 pKa = 10.6TNNDD340 pKa = 3.52DD341 pKa = 3.85EE342 pKa = 4.89TFNLSNEE349 pKa = 4.38CFPDD353 pKa = 3.78DD354 pKa = 3.61NRR356 pKa = 11.84AAYY359 pKa = 9.93SSAVGDD365 pKa = 3.96VDD367 pKa = 3.96NDD369 pKa = 4.14GYY371 pKa = 10.43PDD373 pKa = 5.41LIVTNSFDD381 pKa = 3.85QDD383 pKa = 2.86IFFWKK388 pKa = 8.56NTTPQTNNWVKK399 pKa = 11.02LEE401 pKa = 4.07LEE403 pKa = 4.41GTVSNRR409 pKa = 11.84DD410 pKa = 3.46GIGSVIEE417 pKa = 3.86VSVNGEE423 pKa = 3.5KK424 pKa = 10.39QYY426 pKa = 11.44AYY428 pKa = 6.82THH430 pKa = 6.43CGEE433 pKa = 5.26GYY435 pKa = 10.58LSQNSATKK443 pKa = 10.16IIGTGTHH450 pKa = 4.88TTIDD454 pKa = 4.01YY455 pKa = 10.97IKK457 pKa = 9.03VTWLSGIVDD466 pKa = 3.83ILYY469 pKa = 9.84DD470 pKa = 3.51VPVNQKK476 pKa = 9.43IDD478 pKa = 3.76LVEE481 pKa = 4.14GTFPEE486 pKa = 4.51LSVAEE491 pKa = 4.16YY492 pKa = 10.91DD493 pKa = 3.85LQNTEE498 pKa = 5.14LYY500 pKa = 9.31PNPVNDD506 pKa = 3.93NLVVAVNEE514 pKa = 4.1DD515 pKa = 3.37ARR517 pKa = 11.84VEE519 pKa = 4.02IYY521 pKa = 10.24TILGKK526 pKa = 10.83RR527 pKa = 11.84MMTTNVSGQGNNQLDD542 pKa = 4.21VSTLPSGMYY551 pKa = 9.52LVKK554 pKa = 10.41ISADD558 pKa = 3.11NGQTLTRR565 pKa = 11.84KK566 pKa = 9.83LIKK569 pKa = 10.21KK570 pKa = 9.67

Molecular weight: 62.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K1DWP2|A0A2K1DWP2_9FLAO Glycosyltransferase OS=Hanstruepera neustonica OX=1445657 GN=C1T31_11690 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 10.15RR11 pKa = 11.84KK12 pKa = 9.6RR13 pKa = 11.84KK14 pKa = 8.29NKK16 pKa = 9.34HH17 pKa = 4.03GFRR20 pKa = 11.84EE21 pKa = 4.16RR22 pKa = 11.84MATANGRR29 pKa = 11.84KK30 pKa = 8.93VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.09GRR40 pKa = 11.84KK41 pKa = 7.97KK42 pKa = 10.52LSVSSEE48 pKa = 3.87PRR50 pKa = 11.84HH51 pKa = 5.92KK52 pKa = 10.61KK53 pKa = 9.84

Molecular weight: 6.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2739

0

2739

942191

38

3366

344.0

38.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.205 ± 0.046

0.815 ± 0.024

5.984 ± 0.051

6.411 ± 0.052

5.23 ± 0.041

6.369 ± 0.06

1.826 ± 0.021

7.957 ± 0.043

7.21 ± 0.078

9.13 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.234 ± 0.026

6.469 ± 0.049

3.427 ± 0.034

3.483 ± 0.029

3.254 ± 0.031

6.537 ± 0.039

5.959 ± 0.071

6.362 ± 0.037

1.023 ± 0.017

4.116 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski