Staphylococcus phage SP120

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Coventryvirus; Staphylococcus virus SP120

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A494WEA9|A0A494WEA9_9CAUD Phage protein OS=Staphylococcus phage SP120 OX=2495553 GN=ORF_59 PE=4 SV=1
MM1 pKa = 7.32IKK3 pKa = 9.8IYY5 pKa = 10.82KK6 pKa = 9.67NEE8 pKa = 3.86NDD10 pKa = 3.52EE11 pKa = 4.82LEE13 pKa = 4.32CHH15 pKa = 6.0VNYY18 pKa = 10.56AGYY21 pKa = 10.33DD22 pKa = 3.51FKK24 pKa = 11.25FQCIKK29 pKa = 10.5NGSGATFKK37 pKa = 10.96GSNSTEE43 pKa = 3.78YY44 pKa = 11.27LEE46 pKa = 4.27FEE48 pKa = 5.06SYY50 pKa = 10.64IDD52 pKa = 4.56DD53 pKa = 5.12DD54 pKa = 6.26GEE56 pKa = 4.63ILDD59 pKa = 4.08NLQDD63 pKa = 3.63VMYY66 pKa = 10.21HH67 pKa = 6.17IASVCNWRR75 pKa = 11.84EE76 pKa = 3.91SFGEE80 pKa = 4.02EE81 pKa = 3.58RR82 pKa = 4.2

Molecular weight: 9.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A494WIL3|A0A494WIL3_9CAUD Phage protein OS=Staphylococcus phage SP120 OX=2495553 GN=ORF_54 PE=4 SV=1
MM1 pKa = 6.63TWKK4 pKa = 10.66DD5 pKa = 2.72IDD7 pKa = 4.33FIGLTKK13 pKa = 10.28SRR15 pKa = 11.84KK16 pKa = 9.66AKK18 pKa = 9.51MIHH21 pKa = 6.45KK22 pKa = 10.25GITPSIALSRR32 pKa = 11.84YY33 pKa = 8.12KK34 pKa = 10.63NYY36 pKa = 10.19WSIDD40 pKa = 3.48EE41 pKa = 4.33IANTKK46 pKa = 10.25PYY48 pKa = 9.29MKK50 pKa = 10.02RR51 pKa = 11.84RR52 pKa = 11.84RR53 pKa = 11.84KK54 pKa = 9.6

Molecular weight: 6.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

12768

47

1039

182.4

20.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.811 ± 0.402

0.462 ± 0.077

6.454 ± 0.357

7.456 ± 0.453

4.441 ± 0.209

4.989 ± 0.263

1.606 ± 0.173

7.378 ± 0.306

9.61 ± 0.326

8.051 ± 0.339

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.326 ± 0.119

6.892 ± 0.273

2.475 ± 0.225

3.752 ± 0.174

3.861 ± 0.205

6.039 ± 0.293

6.297 ± 0.344

6.61 ± 0.227

1.057 ± 0.142

4.433 ± 0.315
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski