Amino acid dipepetide frequency for Staphylococcus phage SP120

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.115AlaAla: 2.115 ± 0.405
0.235AlaCys: 0.235 ± 0.113
3.995AlaAsp: 3.995 ± 0.527
4.308AlaGlu: 4.308 ± 0.721
2.506AlaPhe: 2.506 ± 0.475
2.976AlaGly: 2.976 ± 0.572
0.862AlaHis: 0.862 ± 0.236
5.953AlaIle: 5.953 ± 1.036
5.64AlaLys: 5.64 ± 0.798
5.091AlaLeu: 5.091 ± 0.675
1.018AlaMet: 1.018 ± 0.285
4.621AlaAsn: 4.621 ± 0.628
0.862AlaPro: 0.862 ± 0.216
2.428AlaGln: 2.428 ± 0.473
2.35AlaArg: 2.35 ± 0.449
2.663AlaSer: 2.663 ± 0.578
4.7AlaThr: 4.7 ± 0.579
3.133AlaVal: 3.133 ± 0.531
0.548AlaTrp: 0.548 ± 0.197
2.115AlaTyr: 2.115 ± 0.388
0.0AlaXaa: 0.0 ± 0.0
Cys
0.078CysAla: 0.078 ± 0.084
0.0CysCys: 0.0 ± 0.0
0.392CysAsp: 0.392 ± 0.197
0.157CysGlu: 0.157 ± 0.118
0.0CysPhe: 0.0 ± 0.0
0.313CysGly: 0.313 ± 0.158
0.157CysHis: 0.157 ± 0.099
0.235CysIle: 0.235 ± 0.107
0.313CysLys: 0.313 ± 0.149
0.47CysLeu: 0.47 ± 0.195
0.235CysMet: 0.235 ± 0.133
0.627CysAsn: 0.627 ± 0.225
0.0CysPro: 0.0 ± 0.0
0.078CysGln: 0.078 ± 0.077
0.392CysArg: 0.392 ± 0.191
0.392CysSer: 0.392 ± 0.16
0.392CysThr: 0.392 ± 0.163
0.078CysVal: 0.078 ± 0.078
0.078CysTrp: 0.078 ± 0.065
0.235CysTyr: 0.235 ± 0.118
0.0CysXaa: 0.0 ± 0.0
Asp
3.211AspAla: 3.211 ± 0.515
0.157AspCys: 0.157 ± 0.107
5.561AspAsp: 5.561 ± 0.825
5.796AspGlu: 5.796 ± 0.814
3.446AspPhe: 3.446 ± 0.564
3.681AspGly: 3.681 ± 0.458
0.783AspHis: 0.783 ± 0.268
4.7AspIle: 4.7 ± 0.596
5.953AspLys: 5.953 ± 0.841
6.031AspLeu: 6.031 ± 0.79
1.88AspMet: 1.88 ± 0.422
4.465AspAsn: 4.465 ± 0.564
1.175AspPro: 1.175 ± 0.216
0.862AspGln: 0.862 ± 0.284
1.645AspArg: 1.645 ± 0.284
4.23AspSer: 4.23 ± 0.563
2.663AspThr: 2.663 ± 0.479
4.386AspVal: 4.386 ± 0.487
0.627AspTrp: 0.627 ± 0.185
3.29AspTyr: 3.29 ± 0.617
0.0AspXaa: 0.0 ± 0.0
Glu
4.935GluAla: 4.935 ± 0.581
0.705GluCys: 0.705 ± 0.192
3.29GluAsp: 3.29 ± 0.592
6.971GluGlu: 6.971 ± 1.17
4.23GluPhe: 4.23 ± 0.654
3.133GluGly: 3.133 ± 0.464
0.627GluHis: 0.627 ± 0.226
5.405GluIle: 5.405 ± 0.717
5.64GluLys: 5.64 ± 0.729
6.344GluLeu: 6.344 ± 0.754
3.055GluMet: 3.055 ± 0.441
4.621GluAsn: 4.621 ± 0.602
1.175GluPro: 1.175 ± 0.287
3.446GluGln: 3.446 ± 0.631
4.151GluArg: 4.151 ± 0.679
3.838GluSer: 3.838 ± 0.628
2.976GluThr: 2.976 ± 0.537
5.875GluVal: 5.875 ± 0.728
0.548GluTrp: 0.548 ± 0.19
3.603GluTyr: 3.603 ± 0.597
0.0GluXaa: 0.0 ± 0.0
Phe
2.506PheAla: 2.506 ± 0.448
0.235PheCys: 0.235 ± 0.135
2.898PheAsp: 2.898 ± 0.464
3.916PheGlu: 3.916 ± 0.577
1.802PhePhe: 1.802 ± 0.319
2.82PheGly: 2.82 ± 0.44
0.392PheHis: 0.392 ± 0.172
3.681PheIle: 3.681 ± 0.444
4.778PheLys: 4.778 ± 0.458
3.055PheLeu: 3.055 ± 0.637
1.097PheMet: 1.097 ± 0.262
3.368PheAsn: 3.368 ± 0.47
0.783PhePro: 0.783 ± 0.244
0.862PheGln: 0.862 ± 0.261
1.488PheArg: 1.488 ± 0.293
2.506PheSer: 2.506 ± 0.546
2.428PheThr: 2.428 ± 0.331
3.603PheVal: 3.603 ± 0.645
0.392PheTrp: 0.392 ± 0.17
1.802PheTyr: 1.802 ± 0.38
0.0PheXaa: 0.0 ± 0.0
Gly
2.506GlyAla: 2.506 ± 0.772
0.392GlyCys: 0.392 ± 0.181
2.271GlyAsp: 2.271 ± 0.386
4.151GlyGlu: 4.151 ± 0.589
2.585GlyPhe: 2.585 ± 0.532
1.802GlyGly: 1.802 ± 0.368
0.862GlyHis: 0.862 ± 0.208
3.838GlyIle: 3.838 ± 0.466
4.543GlyLys: 4.543 ± 0.709
4.308GlyLeu: 4.308 ± 0.618
0.862GlyMet: 0.862 ± 0.25
2.428GlyAsn: 2.428 ± 0.413
0.313GlyPro: 0.313 ± 0.231
1.645GlyGln: 1.645 ± 0.362
2.271GlyArg: 2.271 ± 0.382
2.898GlySer: 2.898 ± 0.368
3.838GlyThr: 3.838 ± 0.416
4.621GlyVal: 4.621 ± 0.674
0.313GlyTrp: 0.313 ± 0.163
3.446GlyTyr: 3.446 ± 0.53
0.0GlyXaa: 0.0 ± 0.0
His
0.313HisAla: 0.313 ± 0.145
0.392HisCys: 0.392 ± 0.159
0.94HisAsp: 0.94 ± 0.265
0.705HisGlu: 0.705 ± 0.232
1.097HisPhe: 1.097 ± 0.323
1.332HisGly: 1.332 ± 0.322
0.313HisHis: 0.313 ± 0.145
1.253HisIle: 1.253 ± 0.374
1.097HisLys: 1.097 ± 0.302
1.723HisLeu: 1.723 ± 0.314
0.313HisMet: 0.313 ± 0.142
1.018HisAsn: 1.018 ± 0.298
0.392HisPro: 0.392 ± 0.181
1.175HisGln: 1.175 ± 0.34
0.313HisArg: 0.313 ± 0.203
0.705HisSer: 0.705 ± 0.234
0.548HisThr: 0.548 ± 0.23
1.175HisVal: 1.175 ± 0.327
0.157HisTrp: 0.157 ± 0.12
1.097HisTyr: 1.097 ± 0.299
0.0HisXaa: 0.0 ± 0.0
Ile
4.7IleAla: 4.7 ± 0.717
0.313IleCys: 0.313 ± 0.142
6.266IleAsp: 6.266 ± 0.591
4.778IleGlu: 4.778 ± 0.757
2.741IlePhe: 2.741 ± 0.519
3.838IleGly: 3.838 ± 0.866
0.94IleHis: 0.94 ± 0.282
3.603IleIle: 3.603 ± 0.516
8.381IleLys: 8.381 ± 0.824
4.308IleLeu: 4.308 ± 0.574
0.94IleMet: 0.94 ± 0.347
6.031IleAsn: 6.031 ± 0.677
3.368IlePro: 3.368 ± 0.548
2.663IleGln: 2.663 ± 0.442
2.663IleArg: 2.663 ± 0.498
4.073IleSer: 4.073 ± 0.608
5.091IleThr: 5.091 ± 0.641
4.543IleVal: 4.543 ± 0.506
0.94IleTrp: 0.94 ± 0.301
3.603IleTyr: 3.603 ± 0.595
0.0IleXaa: 0.0 ± 0.0
Lys
6.031LysAla: 6.031 ± 0.702
0.235LysCys: 0.235 ± 0.137
6.11LysAsp: 6.11 ± 0.655
7.441LysGlu: 7.441 ± 1.032
3.838LysPhe: 3.838 ± 0.556
5.248LysGly: 5.248 ± 0.608
1.018LysHis: 1.018 ± 0.281
8.146LysIle: 8.146 ± 0.781
8.381LysLys: 8.381 ± 0.916
7.598LysLeu: 7.598 ± 0.638
2.976LysMet: 2.976 ± 0.459
5.091LysAsn: 5.091 ± 0.577
2.35LysPro: 2.35 ± 0.721
5.796LysGln: 5.796 ± 0.656
3.995LysArg: 3.995 ± 0.545
5.405LysSer: 5.405 ± 0.726
5.483LysThr: 5.483 ± 0.769
6.266LysVal: 6.266 ± 0.669
0.862LysTrp: 0.862 ± 0.241
3.838LysTyr: 3.838 ± 0.641
0.0LysXaa: 0.0 ± 0.0
Leu
4.386LeuAla: 4.386 ± 0.577
0.157LeuCys: 0.157 ± 0.156
5.718LeuAsp: 5.718 ± 0.686
6.188LeuGlu: 6.188 ± 0.667
3.681LeuPhe: 3.681 ± 0.486
3.133LeuGly: 3.133 ± 0.601
1.488LeuHis: 1.488 ± 0.356
5.483LeuIle: 5.483 ± 0.65
8.303LeuLys: 8.303 ± 0.816
6.031LeuLeu: 6.031 ± 0.756
2.115LeuMet: 2.115 ± 0.372
6.031LeuAsn: 6.031 ± 0.757
2.193LeuPro: 2.193 ± 0.39
3.055LeuGln: 3.055 ± 0.46
3.29LeuArg: 3.29 ± 0.624
5.326LeuSer: 5.326 ± 0.875
6.501LeuThr: 6.501 ± 0.747
3.838LeuVal: 3.838 ± 0.614
1.175LeuTrp: 1.175 ± 0.371
2.428LeuTyr: 2.428 ± 0.403
0.0LeuXaa: 0.0 ± 0.0
Met
1.488MetAla: 1.488 ± 0.354
0.078MetCys: 0.078 ± 0.07
1.41MetAsp: 1.41 ± 0.291
2.506MetGlu: 2.506 ± 0.501
0.94MetPhe: 0.94 ± 0.247
0.783MetGly: 0.783 ± 0.203
0.548MetHis: 0.548 ± 0.195
1.88MetIle: 1.88 ± 0.332
1.958MetLys: 1.958 ± 0.353
1.802MetLeu: 1.802 ± 0.353
0.313MetMet: 0.313 ± 0.135
1.175MetAsn: 1.175 ± 0.32
0.548MetPro: 0.548 ± 0.239
0.862MetGln: 0.862 ± 0.239
0.94MetArg: 0.94 ± 0.271
1.645MetSer: 1.645 ± 0.335
1.723MetThr: 1.723 ± 0.38
1.332MetVal: 1.332 ± 0.344
0.157MetTrp: 0.157 ± 0.102
1.175MetTyr: 1.175 ± 0.287
0.0MetXaa: 0.0 ± 0.0
Asn
4.856AsnAla: 4.856 ± 0.673
0.548AsnCys: 0.548 ± 0.219
4.7AsnAsp: 4.7 ± 0.793
4.778AsnGlu: 4.778 ± 0.602
2.271AsnPhe: 2.271 ± 0.382
5.013AsnGly: 5.013 ± 0.643
1.175AsnHis: 1.175 ± 0.296
3.368AsnIle: 3.368 ± 0.551
6.814AsnLys: 6.814 ± 0.703
5.17AsnLeu: 5.17 ± 0.691
1.488AsnMet: 1.488 ± 0.321
5.405AsnAsn: 5.405 ± 0.882
2.193AsnPro: 2.193 ± 0.413
1.88AsnGln: 1.88 ± 0.439
2.115AsnArg: 2.115 ± 0.374
4.151AsnSer: 4.151 ± 0.582
4.543AsnThr: 4.543 ± 0.611
4.778AsnVal: 4.778 ± 0.64
0.94AsnTrp: 0.94 ± 0.263
2.741AsnTyr: 2.741 ± 0.495
0.0AsnXaa: 0.0 ± 0.0
Pro
1.41ProAla: 1.41 ± 0.294
0.157ProCys: 0.157 ± 0.108
1.332ProAsp: 1.332 ± 0.333
1.488ProGlu: 1.488 ± 0.347
1.253ProPhe: 1.253 ± 0.312
0.627ProGly: 0.627 ± 0.251
0.392ProHis: 0.392 ± 0.153
1.958ProIle: 1.958 ± 0.555
2.82ProLys: 2.82 ± 0.594
1.332ProLeu: 1.332 ± 0.348
0.548ProMet: 0.548 ± 0.193
2.115ProAsn: 2.115 ± 0.326
0.783ProPro: 0.783 ± 0.221
1.097ProGln: 1.097 ± 0.306
1.018ProArg: 1.018 ± 0.273
1.958ProSer: 1.958 ± 0.375
1.645ProThr: 1.645 ± 0.343
1.802ProVal: 1.802 ± 0.383
0.0ProTrp: 0.0 ± 0.0
1.018ProTyr: 1.018 ± 0.271
0.0ProXaa: 0.0 ± 0.0
Gln
2.741GlnAla: 2.741 ± 0.477
0.392GlnCys: 0.392 ± 0.17
1.645GlnAsp: 1.645 ± 0.302
1.88GlnGlu: 1.88 ± 0.498
1.802GlnPhe: 1.802 ± 0.323
1.332GlnGly: 1.332 ± 0.308
0.548GlnHis: 0.548 ± 0.247
2.35GlnIle: 2.35 ± 0.384
4.465GlnLys: 4.465 ± 0.61
3.446GlnLeu: 3.446 ± 0.525
0.313GlnMet: 0.313 ± 0.178
3.211GlnAsn: 3.211 ± 0.496
0.705GlnPro: 0.705 ± 0.262
1.567GlnGln: 1.567 ± 0.457
1.332GlnArg: 1.332 ± 0.268
2.741GlnSer: 2.741 ± 0.441
2.82GlnThr: 2.82 ± 0.436
2.506GlnVal: 2.506 ± 0.472
0.235GlnTrp: 0.235 ± 0.123
1.488GlnTyr: 1.488 ± 0.321
0.0GlnXaa: 0.0 ± 0.0
Arg
1.723ArgAla: 1.723 ± 0.357
0.157ArgCys: 0.157 ± 0.102
2.037ArgAsp: 2.037 ± 0.394
2.428ArgGlu: 2.428 ± 0.417
1.802ArgPhe: 1.802 ± 0.311
1.567ArgGly: 1.567 ± 0.343
0.862ArgHis: 0.862 ± 0.286
4.073ArgIle: 4.073 ± 0.552
3.133ArgLys: 3.133 ± 0.522
4.151ArgLeu: 4.151 ± 0.584
1.253ArgMet: 1.253 ± 0.281
1.958ArgAsn: 1.958 ± 0.293
0.705ArgPro: 0.705 ± 0.263
1.802ArgGln: 1.802 ± 0.311
1.41ArgArg: 1.41 ± 0.298
1.88ArgSer: 1.88 ± 0.309
2.428ArgThr: 2.428 ± 0.544
2.271ArgVal: 2.271 ± 0.368
0.47ArgTrp: 0.47 ± 0.187
2.506ArgTyr: 2.506 ± 0.389
0.0ArgXaa: 0.0 ± 0.0
Ser
3.29SerAla: 3.29 ± 0.59
0.0SerCys: 0.0 ± 0.0
3.368SerAsp: 3.368 ± 0.439
4.308SerGlu: 4.308 ± 0.493
2.506SerPhe: 2.506 ± 0.659
3.76SerGly: 3.76 ± 0.523
1.41SerHis: 1.41 ± 0.323
5.405SerIle: 5.405 ± 0.822
5.796SerLys: 5.796 ± 0.748
5.483SerLeu: 5.483 ± 0.773
1.175SerMet: 1.175 ± 0.287
4.465SerAsn: 4.465 ± 0.613
1.488SerPro: 1.488 ± 0.314
1.488SerGln: 1.488 ± 0.289
2.82SerArg: 2.82 ± 0.424
3.29SerSer: 3.29 ± 0.721
3.603SerThr: 3.603 ± 0.519
3.838SerVal: 3.838 ± 0.48
0.47SerTrp: 0.47 ± 0.215
2.35SerTyr: 2.35 ± 0.424
0.0SerXaa: 0.0 ± 0.0
Thr
3.838ThrAla: 3.838 ± 0.491
0.078ThrCys: 0.078 ± 0.083
4.073ThrAsp: 4.073 ± 0.623
3.446ThrGlu: 3.446 ± 0.581
2.585ThrPhe: 2.585 ± 0.459
3.211ThrGly: 3.211 ± 0.521
1.723ThrHis: 1.723 ± 0.286
4.778ThrIle: 4.778 ± 0.686
7.128ThrLys: 7.128 ± 0.766
5.326ThrLeu: 5.326 ± 0.641
0.94ThrMet: 0.94 ± 0.277
3.211ThrAsn: 3.211 ± 0.546
2.193ThrPro: 2.193 ± 0.403
1.958ThrGln: 1.958 ± 0.382
2.271ThrArg: 2.271 ± 0.46
3.916ThrSer: 3.916 ± 0.553
4.308ThrThr: 4.308 ± 0.714
4.543ThrVal: 4.543 ± 0.849
0.862ThrTrp: 0.862 ± 0.329
2.585ThrTyr: 2.585 ± 0.454
0.0ThrXaa: 0.0 ± 0.0
Val
4.308ValAla: 4.308 ± 0.505
0.157ValCys: 0.157 ± 0.11
4.935ValAsp: 4.935 ± 0.511
5.718ValGlu: 5.718 ± 0.649
2.976ValPhe: 2.976 ± 0.548
2.898ValGly: 2.898 ± 0.515
0.783ValHis: 0.783 ± 0.226
5.091ValIle: 5.091 ± 0.663
5.405ValLys: 5.405 ± 0.532
4.621ValLeu: 4.621 ± 0.61
1.253ValMet: 1.253 ± 0.317
5.17ValAsn: 5.17 ± 0.635
2.115ValPro: 2.115 ± 0.392
2.193ValGln: 2.193 ± 0.37
1.802ValArg: 1.802 ± 0.308
5.17ValSer: 5.17 ± 0.567
4.073ValThr: 4.073 ± 0.635
4.151ValVal: 4.151 ± 0.663
0.783ValTrp: 0.783 ± 0.212
2.506ValTyr: 2.506 ± 0.397
0.0ValXaa: 0.0 ± 0.0
Trp
0.94TrpAla: 0.94 ± 0.275
0.078TrpCys: 0.078 ± 0.078
0.548TrpAsp: 0.548 ± 0.196
0.47TrpGlu: 0.47 ± 0.174
0.392TrpPhe: 0.392 ± 0.177
0.392TrpGly: 0.392 ± 0.206
0.235TrpHis: 0.235 ± 0.134
0.392TrpIle: 0.392 ± 0.156
0.627TrpLys: 0.627 ± 0.224
0.783TrpLeu: 0.783 ± 0.247
0.235TrpMet: 0.235 ± 0.147
1.175TrpAsn: 1.175 ± 0.605
0.078TrpPro: 0.078 ± 0.077
0.548TrpGln: 0.548 ± 0.262
0.313TrpArg: 0.313 ± 0.133
1.253TrpSer: 1.253 ± 0.332
0.705TrpThr: 0.705 ± 0.25
0.783TrpVal: 0.783 ± 0.226
0.0TrpTrp: 0.0 ± 0.0
0.627TrpTyr: 0.627 ± 0.21
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.741TyrAla: 2.741 ± 0.419
0.157TyrCys: 0.157 ± 0.114
3.055TyrAsp: 3.055 ± 0.479
3.133TyrGlu: 3.133 ± 0.46
1.958TyrPhe: 1.958 ± 0.432
2.037TyrGly: 2.037 ± 0.353
0.94TyrHis: 0.94 ± 0.303
2.193TyrIle: 2.193 ± 0.422
4.935TyrLys: 4.935 ± 0.698
3.446TyrLeu: 3.446 ± 0.543
1.175TyrMet: 1.175 ± 0.336
2.741TyrAsn: 2.741 ± 0.408
1.332TyrPro: 1.332 ± 0.287
2.115TyrGln: 2.115 ± 0.449
2.037TyrArg: 2.037 ± 0.366
2.35TyrSer: 2.35 ± 0.411
2.506TyrThr: 2.506 ± 0.538
2.585TyrVal: 2.585 ± 0.409
1.018TyrTrp: 1.018 ± 0.323
1.88TyrTyr: 1.88 ± 0.439
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 70 proteins (12768 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski