Candidatus Defluviicoccus seviourii
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3221 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A564WDF9|A0A564WDF9_9PROT Vitamin B12-dependent ribonucleotide reductase OS=Candidatus Defluviicoccus seviourii OX=2565273 GN=nrdJ PE=3 SV=1
MM1 pKa = 7.25 MATEE5 pKa = 3.97 VTGFIEE11 pKa = 4.25 SLLRR15 pKa = 11.84 SIGMLFGSAEE25 pKa = 4.17 ADD27 pKa = 3.57 SATDD31 pKa = 3.45 GLFVVAIGAALVLIVGSWFFRR52 pKa = 11.84 PGTVDD57 pKa = 3.0 SSDD60 pKa = 3.47 YY61 pKa = 10.97 GVNHH65 pKa = 6.83 FDD67 pKa = 4.21 HH68 pKa = 7.08 GGCFGGDD75 pKa = 3.3 GSDD78 pKa = 3.48 CGGDD82 pKa = 3.84 GGCGDD87 pKa = 4.04
Molecular weight: 8.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.732
IPC2_protein 3.757
IPC_protein 3.694
Toseland 3.49
ProMoST 3.808
Dawson 3.719
Bjellqvist 3.961
Wikipedia 3.706
Rodwell 3.541
Grimsley 3.401
Solomon 3.694
Lehninger 3.656
Nozaki 3.859
DTASelect 4.113
Thurlkill 3.579
EMBOSS 3.719
Sillero 3.834
Patrickios 0.693
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.804
Protein with the highest isoelectric point:
>tr|A0A564WFC1|A0A564WFC1_9PROT Peptide methionine sulfoxide reductase MsrA OS=Candidatus Defluviicoccus seviourii OX=2565273 GN=msrA PE=3 SV=1
MM1 pKa = 7.16 NAHH4 pKa = 7.31 AIEE7 pKa = 4.39 LVAKK11 pKa = 10.3 AFLAGFIVAVPVGAIGAMCLRR32 pKa = 11.84 RR33 pKa = 11.84 AFAGRR38 pKa = 11.84 LSDD41 pKa = 4.2 ALVTGFGAAVADD53 pKa = 4.34 ALWAAAAILGLSLITRR69 pKa = 11.84 YY70 pKa = 9.6 VVEE73 pKa = 4.2 HH74 pKa = 7.34 DD75 pKa = 3.61 GPARR79 pKa = 11.84 VIGGLALIAVGAKK92 pKa = 9.07 MIHH95 pKa = 5.61 ARR97 pKa = 11.84 RR98 pKa = 11.84 RR99 pKa = 11.84 QLVPLAAEE107 pKa = 4.13 VAAPAVYY114 pKa = 9.71 RR115 pKa = 11.84 WRR117 pKa = 11.84 AWCRR121 pKa = 11.84 DD122 pKa = 3.02 AATGFGLTIINPATFIGFIAVCAGLGLFVSNLDD155 pKa = 3.48 TLMSKK160 pKa = 9.28 WFIVFGAFAGSMLWWVILSLTASAVRR186 pKa = 11.84 HH187 pKa = 6.24 RR188 pKa = 11.84 IPLQALAMLNAGLGIVVAVLGVVSLLSAFGAA219 pKa = 3.99
Molecular weight: 22.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 9.516
IPC_protein 10.482
Toseland 10.584
ProMoST 10.379
Dawson 10.687
Bjellqvist 10.467
Wikipedia 10.935
Rodwell 10.745
Grimsley 10.745
Solomon 10.847
Lehninger 10.818
Nozaki 10.613
DTASelect 10.452
Thurlkill 10.599
EMBOSS 10.994
Sillero 10.643
Patrickios 10.526
IPC_peptide 10.847
IPC2_peptide 9.838
IPC2.peptide.svr19 8.536
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3221
0
3221
997156
20
2394
309.6
33.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.481 ± 0.07
1.05 ± 0.015
5.489 ± 0.034
5.637 ± 0.038
3.565 ± 0.028
8.495 ± 0.043
2.08 ± 0.022
4.887 ± 0.028
2.766 ± 0.028
10.438 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.125 ± 0.022
2.316 ± 0.022
5.407 ± 0.035
3.16 ± 0.025
7.807 ± 0.043
5.001 ± 0.032
5.214 ± 0.029
7.613 ± 0.037
1.34 ± 0.02
2.133 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here