Streptomyces monashensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8549 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S2Q2H8|A0A1S2Q2H8_9ACTN T2SSF domain-containing protein OS=Streptomyces monashensis OX=1678012 GN=BIV23_27265 PE=4 SV=1
MM1 pKa = 7.53SAPHH5 pKa = 6.51TDD7 pKa = 3.36VEE9 pKa = 4.44QVEE12 pKa = 4.42AANTAFYY19 pKa = 10.82EE20 pKa = 4.25AMEE23 pKa = 4.84CGDD26 pKa = 4.03FEE28 pKa = 4.58EE29 pKa = 6.09LSSLWLSPADD39 pKa = 3.54LGVDD43 pKa = 3.75EE44 pKa = 5.74EE45 pKa = 4.75YY46 pKa = 10.73HH47 pKa = 6.99DD48 pKa = 4.52PADD51 pKa = 3.68TGVISCVHH59 pKa = 6.58PGWPVLTGRR68 pKa = 11.84GEE70 pKa = 4.16VLRR73 pKa = 11.84SYY75 pKa = 11.35ALIMANTDD83 pKa = 3.58YY84 pKa = 11.13IQFFLTDD91 pKa = 2.76VHH93 pKa = 8.04VSVTGDD99 pKa = 3.33TALVTCTEE107 pKa = 4.71NILSGGPAPADD118 pKa = 3.54GGEE121 pKa = 4.13LGPLVGQLVVATNVFRR137 pKa = 11.84RR138 pKa = 11.84TPAGWKK144 pKa = 8.81IWSHH148 pKa = 5.69HH149 pKa = 6.45ASPVMAEE156 pKa = 4.09SEE158 pKa = 4.11DD159 pKa = 4.08AEE161 pKa = 4.57VVEE164 pKa = 6.15DD165 pKa = 4.03MDD167 pKa = 6.09AMDD170 pKa = 5.96DD171 pKa = 3.85EE172 pKa = 5.6PGTNGTNPDD181 pKa = 4.08EE182 pKa = 4.9GPLGG186 pKa = 3.78

Molecular weight: 19.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S2QCL0|A0A1S2QCL0_9ACTN Type-5 uracil-DNA glycosylase OS=Streptomyces monashensis OX=1678012 GN=BIV23_22500 PE=3 SV=1
MM1 pKa = 7.68RR2 pKa = 11.84LISIWASGATIGASISPMRR21 pKa = 11.84PGSASSSPSGQGVPAQGPPATVTRR45 pKa = 11.84PAASRR50 pKa = 11.84STRR53 pKa = 11.84VKK55 pKa = 10.37LLSSASRR62 pKa = 11.84TKK64 pKa = 10.34SSRR67 pKa = 11.84RR68 pKa = 11.84SGGGPGTGVV77 pKa = 2.85

Molecular weight: 7.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8549

0

8549

2756139

29

6126

322.4

34.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.801 ± 0.04

0.824 ± 0.008

5.923 ± 0.022

5.461 ± 0.029

2.72 ± 0.015

9.418 ± 0.029

2.442 ± 0.013

3.046 ± 0.018

2.015 ± 0.022

10.498 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.687 ± 0.009

1.728 ± 0.015

6.107 ± 0.027

2.811 ± 0.016

8.172 ± 0.032

5.014 ± 0.022

6.223 ± 0.023

8.456 ± 0.026

1.533 ± 0.011

2.119 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski