Rhizoclosmatium globosum

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Chytridiomycota; Chytridiomycota incertae sedis; Chytridiomycetes; Chytridiales; Chytriomycetaceae; Rhizoclosmatium

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16725 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y2C6U4|A0A1Y2C6U4_9FUNG Uncharacterized protein OS=Rhizoclosmatium globosum OX=329046 GN=BCR33DRAFT_717942 PE=4 SV=1
MM1 pKa = 7.49SNSFLFSLASIIARR15 pKa = 11.84LEE17 pKa = 4.08EE18 pKa = 4.73DD19 pKa = 3.55PSTHH23 pKa = 5.03YY24 pKa = 9.64TADD27 pKa = 3.75CVSVCSEE34 pKa = 4.26GDD36 pKa = 3.32SSSSSEE42 pKa = 4.45SDD44 pKa = 2.87EE45 pKa = 5.84DD46 pKa = 5.06NNNDD50 pKa = 4.78DD51 pKa = 5.15ANQSSDD57 pKa = 3.02IAEE60 pKa = 4.88DD61 pKa = 3.38EE62 pKa = 4.73LEE64 pKa = 3.93CHH66 pKa = 5.99EE67 pKa = 4.13RR68 pKa = 11.84VEE70 pKa = 4.6AVKK73 pKa = 10.17HH74 pKa = 5.33AQTATNGGQQLYY86 pKa = 10.75ALWQDD91 pKa = 3.38ADD93 pKa = 4.62LEE95 pKa = 4.68LSNQTDD101 pKa = 3.93NDD103 pKa = 3.77SSSYY107 pKa = 10.53ISNLMLRR114 pKa = 11.84IKK116 pKa = 10.39YY117 pKa = 10.24AEE119 pKa = 3.96SRR121 pKa = 11.84QNIANNDD128 pKa = 3.11GDD130 pKa = 5.42LDD132 pKa = 3.89VDD134 pKa = 5.97DD135 pKa = 5.84FINWDD140 pKa = 4.24LYY142 pKa = 10.26WSSAHH147 pKa = 5.52QAGLRR152 pKa = 11.84EE153 pKa = 4.01AMQVSEE159 pKa = 4.27LSVYY163 pKa = 10.35AVSGTWDD170 pKa = 3.42SEE172 pKa = 4.1IEE174 pKa = 4.25AKK176 pKa = 10.4NLPSLFSSISFYY188 pKa = 11.67

Molecular weight: 20.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y2BXD3|A0A1Y2BXD3_9FUNG NAD dependent epimerase/dehydratase family protein OS=Rhizoclosmatium globosum OX=329046 GN=BCR33DRAFT_720256 PE=4 SV=1
MM1 pKa = 7.47LRR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84QLQRR9 pKa = 11.84RR10 pKa = 11.84LLQRR14 pKa = 11.84SQRR17 pKa = 11.84LLFQKK22 pKa = 10.28RR23 pKa = 11.84SPRR26 pKa = 11.84QRR28 pKa = 11.84RR29 pKa = 11.84PLFPKK34 pKa = 10.14PNQLPKK40 pKa = 10.45RR41 pKa = 11.84LQRR44 pKa = 11.84SQLSPLLLKK53 pKa = 10.73RR54 pKa = 11.84KK55 pKa = 8.16LQKK58 pKa = 10.0SRR60 pKa = 11.84KK61 pKa = 7.51LPRR64 pKa = 11.84RR65 pKa = 11.84RR66 pKa = 11.84RR67 pKa = 11.84LLPRR71 pKa = 11.84EE72 pKa = 3.93QLHH75 pKa = 6.81LEE77 pKa = 3.92AKK79 pKa = 9.27LRR81 pKa = 11.84RR82 pKa = 11.84RR83 pKa = 11.84RR84 pKa = 11.84RR85 pKa = 11.84MLQRR89 pKa = 11.84RR90 pKa = 11.84LLLQKK95 pKa = 10.58KK96 pKa = 9.14PLLHH100 pKa = 6.12QKK102 pKa = 10.25SSSFSKK108 pKa = 10.65KK109 pKa = 9.8SASDD113 pKa = 3.33HH114 pKa = 6.02

Molecular weight: 14.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16725

0

16725

6640880

49

5506

397.1

43.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.874 ± 0.022

1.468 ± 0.009

5.194 ± 0.014

5.791 ± 0.021

4.213 ± 0.013

6.084 ± 0.019

2.221 ± 0.01

5.383 ± 0.016

5.562 ± 0.018

9.446 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.223 ± 0.008

4.328 ± 0.012

5.33 ± 0.021

4.047 ± 0.017

4.849 ± 0.017

8.671 ± 0.027

6.381 ± 0.028

6.908 ± 0.016

1.233 ± 0.008

2.794 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski