Lentibacillus halodurans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Lentibacillus

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3521 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I0W2M1|A0A1I0W2M1_9BACI EDD domain protein DegV family OS=Lentibacillus halodurans OX=237679 GN=SAMN04488072_102113 PE=4 SV=1
MM1 pKa = 6.84QKK3 pKa = 10.78KK4 pKa = 8.52MLIILGSIVVLFAALYY20 pKa = 9.16FVIDD24 pKa = 4.07YY25 pKa = 10.63KK26 pKa = 11.04NQQAIGDD33 pKa = 3.92NEE35 pKa = 4.01NPYY38 pKa = 11.11GKK40 pKa = 9.87DD41 pKa = 3.34DD42 pKa = 4.74LEE44 pKa = 4.34QATIDD49 pKa = 4.17QLDD52 pKa = 3.86DD53 pKa = 4.06PNYY56 pKa = 10.03QNQIVPDD63 pKa = 3.84EE64 pKa = 4.61LSEE67 pKa = 3.96QLHH70 pKa = 6.26NGEE73 pKa = 4.39DD74 pKa = 3.67MVVYY78 pKa = 9.43FYY80 pKa = 11.32DD81 pKa = 3.92PTCPHH86 pKa = 6.31CQEE89 pKa = 3.98LTPRR93 pKa = 11.84LVPIAEE99 pKa = 4.1EE100 pKa = 4.0MDD102 pKa = 3.25VDD104 pKa = 4.1MKK106 pKa = 11.12KK107 pKa = 10.94LNLLEE112 pKa = 5.99FRR114 pKa = 11.84DD115 pKa = 3.53AWDD118 pKa = 3.18QYY120 pKa = 11.22GIEE123 pKa = 4.23STPTLIYY130 pKa = 10.41FEE132 pKa = 4.76NGEE135 pKa = 4.06EE136 pKa = 3.97VDD138 pKa = 4.14RR139 pKa = 11.84VNGAQQDD146 pKa = 4.08DD147 pKa = 3.7IFRR150 pKa = 11.84AFFNEE155 pKa = 3.75YY156 pKa = 10.48VMNDD160 pKa = 3.25SEE162 pKa = 5.36NNDD165 pKa = 3.43EE166 pKa = 4.24QASS169 pKa = 3.69

Molecular weight: 19.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I0ZWT8|A0A1I0ZWT8_9BACI Uncharacterized membrane protein YphA DoxX/SURF4 family OS=Lentibacillus halodurans OX=237679 GN=SAMN04488072_11455 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.02KK14 pKa = 8.47VHH16 pKa = 5.63GFRR19 pKa = 11.84SRR21 pKa = 11.84MKK23 pKa = 10.63SKK25 pKa = 10.79DD26 pKa = 2.81GRR28 pKa = 11.84QVLKK32 pKa = 10.25RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.96GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3521

0

3521

1011085

32

2316

287.2

32.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.228 ± 0.042

0.633 ± 0.011

5.85 ± 0.039

7.435 ± 0.043

4.368 ± 0.037

7.06 ± 0.047

2.254 ± 0.024

7.679 ± 0.039

6.079 ± 0.043

9.332 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.004 ± 0.018

4.546 ± 0.029

3.671 ± 0.023

3.919 ± 0.031

4.04 ± 0.028

5.935 ± 0.028

5.527 ± 0.029

6.948 ± 0.037

1.009 ± 0.017

3.483 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski