Amino acid dipepetide frequency for Lentibacillus halodurans

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.423AlaAla: 6.423 ± 0.097
0.611AlaCys: 0.611 ± 0.023
4.172AlaAsp: 4.172 ± 0.072
5.058AlaGlu: 5.058 ± 0.082
3.419AlaPhe: 3.419 ± 0.058
5.84AlaGly: 5.84 ± 0.086
1.321AlaHis: 1.321 ± 0.033
5.771AlaIle: 5.771 ± 0.086
4.208AlaLys: 4.208 ± 0.07
6.995AlaLeu: 6.995 ± 0.091
2.16AlaMet: 2.16 ± 0.047
3.11AlaAsn: 3.11 ± 0.056
2.025AlaPro: 2.025 ± 0.049
2.147AlaGln: 2.147 ± 0.045
2.612AlaArg: 2.612 ± 0.056
4.305AlaSer: 4.305 ± 0.075
3.285AlaThr: 3.285 ± 0.065
5.784AlaVal: 5.784 ± 0.085
0.633AlaTrp: 0.633 ± 0.028
2.402AlaTyr: 2.402 ± 0.05
0.0AlaXaa: 0.0 ± 0.0
Cys
0.392CysAla: 0.392 ± 0.021
0.077CysCys: 0.077 ± 0.009
0.411CysAsp: 0.411 ± 0.019
0.393CysGlu: 0.393 ± 0.019
0.252CysPhe: 0.252 ± 0.016
0.65CysGly: 0.65 ± 0.028
0.209CysHis: 0.209 ± 0.016
0.449CysIle: 0.449 ± 0.02
0.255CysLys: 0.255 ± 0.016
0.566CysLeu: 0.566 ± 0.021
0.181CysMet: 0.181 ± 0.014
0.258CysAsn: 0.258 ± 0.019
0.316CysPro: 0.316 ± 0.019
0.219CysGln: 0.219 ± 0.014
0.285CysArg: 0.285 ± 0.018
0.456CysSer: 0.456 ± 0.02
0.342CysThr: 0.342 ± 0.018
0.343CysVal: 0.343 ± 0.018
0.051CysTrp: 0.051 ± 0.007
0.226CysTyr: 0.226 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
3.814AspAla: 3.814 ± 0.07
0.317AspCys: 0.317 ± 0.017
3.508AspAsp: 3.508 ± 0.067
5.066AspGlu: 5.066 ± 0.082
2.478AspPhe: 2.478 ± 0.051
3.818AspGly: 3.818 ± 0.074
1.499AspHis: 1.499 ± 0.043
4.694AspIle: 4.694 ± 0.07
3.408AspLys: 3.408 ± 0.06
5.156AspLeu: 5.156 ± 0.072
1.744AspMet: 1.744 ± 0.036
2.309AspAsn: 2.309 ± 0.053
2.283AspPro: 2.283 ± 0.055
2.495AspGln: 2.495 ± 0.047
2.536AspArg: 2.536 ± 0.052
2.938AspSer: 2.938 ± 0.059
2.983AspThr: 2.983 ± 0.06
4.371AspVal: 4.371 ± 0.063
0.683AspTrp: 0.683 ± 0.028
2.396AspTyr: 2.396 ± 0.05
0.0AspXaa: 0.0 ± 0.0
Glu
5.426GluAla: 5.426 ± 0.082
0.314GluCys: 0.314 ± 0.018
4.201GluAsp: 4.201 ± 0.076
6.501GluGlu: 6.501 ± 0.102
2.321GluPhe: 2.321 ± 0.052
4.278GluGly: 4.278 ± 0.074
1.708GluHis: 1.708 ± 0.04
5.072GluIle: 5.072 ± 0.08
5.874GluLys: 5.874 ± 0.083
6.834GluLeu: 6.834 ± 0.098
2.447GluMet: 2.447 ± 0.046
4.146GluAsn: 4.146 ± 0.069
2.212GluPro: 2.212 ± 0.047
3.834GluGln: 3.834 ± 0.083
3.454GluArg: 3.454 ± 0.056
3.857GluSer: 3.857 ± 0.07
4.137GluThr: 4.137 ± 0.065
4.695GluVal: 4.695 ± 0.089
0.835GluTrp: 0.835 ± 0.03
2.202GluTyr: 2.202 ± 0.046
0.0GluXaa: 0.0 ± 0.0
Phe
3.032PheAla: 3.032 ± 0.06
0.307PheCys: 0.307 ± 0.016
2.532PheAsp: 2.532 ± 0.046
2.614PheGlu: 2.614 ± 0.052
2.149PhePhe: 2.149 ± 0.06
3.271PheGly: 3.271 ± 0.076
1.021PheHis: 1.021 ± 0.039
3.623PheIle: 3.623 ± 0.08
2.082PheLys: 2.082 ± 0.047
4.256PheLeu: 4.256 ± 0.086
1.355PheMet: 1.355 ± 0.039
1.879PheAsn: 1.879 ± 0.045
1.642PhePro: 1.642 ± 0.04
1.628PheGln: 1.628 ± 0.038
1.546PheArg: 1.546 ± 0.043
3.108PheSer: 3.108 ± 0.057
2.585PheThr: 2.585 ± 0.057
2.939PheVal: 2.939 ± 0.06
0.464PheTrp: 0.464 ± 0.022
1.649PheTyr: 1.649 ± 0.046
0.0PheXaa: 0.0 ± 0.0
Gly
5.034GlyAla: 5.034 ± 0.084
0.574GlyCys: 0.574 ± 0.026
3.618GlyAsp: 3.618 ± 0.067
4.747GlyGlu: 4.747 ± 0.072
3.445GlyPhe: 3.445 ± 0.063
5.07GlyGly: 5.07 ± 0.093
1.491GlyHis: 1.491 ± 0.044
6.146GlyIle: 6.146 ± 0.094
4.59GlyLys: 4.59 ± 0.07
6.544GlyLeu: 6.544 ± 0.099
2.394GlyMet: 2.394 ± 0.053
2.928GlyAsn: 2.928 ± 0.054
1.824GlyPro: 1.824 ± 0.043
2.088GlyGln: 2.088 ± 0.047
2.614GlyArg: 2.614 ± 0.058
4.407GlySer: 4.407 ± 0.07
4.289GlyThr: 4.289 ± 0.069
5.127GlyVal: 5.127 ± 0.092
0.832GlyTrp: 0.832 ± 0.031
2.839GlyTyr: 2.839 ± 0.051
0.0GlyXaa: 0.0 ± 0.0
His
1.479HisAla: 1.479 ± 0.041
0.169HisCys: 0.169 ± 0.014
1.388HisAsp: 1.388 ± 0.04
1.561HisGlu: 1.561 ± 0.04
1.081HisPhe: 1.081 ± 0.035
1.493HisGly: 1.493 ± 0.039
0.824HisHis: 0.824 ± 0.034
1.674HisIle: 1.674 ± 0.044
1.099HisLys: 1.099 ± 0.035
2.169HisLeu: 2.169 ± 0.045
0.618HisMet: 0.618 ± 0.023
0.888HisAsn: 0.888 ± 0.028
1.163HisPro: 1.163 ± 0.036
0.96HisGln: 0.96 ± 0.024
0.935HisArg: 0.935 ± 0.033
1.103HisSer: 1.103 ± 0.035
1.149HisThr: 1.149 ± 0.033
1.561HisVal: 1.561 ± 0.039
0.218HisTrp: 0.218 ± 0.014
1.012HisTyr: 1.012 ± 0.035
0.0HisXaa: 0.0 ± 0.0
Ile
6.258IleAla: 6.258 ± 0.095
0.56IleCys: 0.56 ± 0.022
4.629IleAsp: 4.629 ± 0.076
5.228IleGlu: 5.228 ± 0.083
3.189IlePhe: 3.189 ± 0.069
6.095IleGly: 6.095 ± 0.107
1.776IleHis: 1.776 ± 0.04
5.688IleIle: 5.688 ± 0.095
3.941IleLys: 3.941 ± 0.066
6.843IleLeu: 6.843 ± 0.115
1.994IleMet: 1.994 ± 0.05
3.279IleAsn: 3.279 ± 0.061
3.446IlePro: 3.446 ± 0.051
2.808IleGln: 2.808 ± 0.05
3.158IleArg: 3.158 ± 0.062
4.958IleSer: 4.958 ± 0.08
4.464IleThr: 4.464 ± 0.067
5.35IleVal: 5.35 ± 0.077
0.674IleTrp: 0.674 ± 0.03
2.452IleTyr: 2.452 ± 0.054
0.0IleXaa: 0.0 ± 0.0
Lys
4.289LysAla: 4.289 ± 0.072
0.267LysCys: 0.267 ± 0.019
3.507LysAsp: 3.507 ± 0.059
5.572LysGlu: 5.572 ± 0.075
1.618LysPhe: 1.618 ± 0.042
3.932LysGly: 3.932 ± 0.064
1.498LysHis: 1.498 ± 0.043
3.851LysIle: 3.851 ± 0.062
4.628LysLys: 4.628 ± 0.079
5.144LysLeu: 5.144 ± 0.079
1.926LysMet: 1.926 ± 0.048
2.966LysAsn: 2.966 ± 0.058
2.144LysPro: 2.144 ± 0.047
3.295LysGln: 3.295 ± 0.064
3.11LysArg: 3.11 ± 0.061
3.235LysSer: 3.235 ± 0.059
3.478LysThr: 3.478 ± 0.059
3.798LysVal: 3.798 ± 0.059
0.725LysTrp: 0.725 ± 0.028
1.807LysTyr: 1.807 ± 0.047
0.0LysXaa: 0.0 ± 0.0
Leu
7.048LeuAla: 7.048 ± 0.099
0.534LeuCys: 0.534 ± 0.026
5.205LeuAsp: 5.205 ± 0.074
6.302LeuGlu: 6.302 ± 0.09
4.553LeuPhe: 4.553 ± 0.098
6.383LeuGly: 6.383 ± 0.098
1.969LeuHis: 1.969 ± 0.047
6.99LeuIle: 6.99 ± 0.111
5.73LeuLys: 5.73 ± 0.086
9.367LeuLeu: 9.367 ± 0.124
2.621LeuMet: 2.621 ± 0.054
4.398LeuAsn: 4.398 ± 0.07
3.822LeuPro: 3.822 ± 0.07
3.545LeuGln: 3.545 ± 0.056
3.48LeuArg: 3.48 ± 0.056
6.237LeuSer: 6.237 ± 0.085
5.533LeuThr: 5.533 ± 0.076
5.916LeuVal: 5.916 ± 0.089
0.768LeuTrp: 0.768 ± 0.03
2.917LeuTyr: 2.917 ± 0.064
0.0LeuXaa: 0.0 ± 0.0
Met
2.368MetAla: 2.368 ± 0.052
0.142MetCys: 0.142 ± 0.013
1.85MetAsp: 1.85 ± 0.041
2.148MetGlu: 2.148 ± 0.046
1.121MetPhe: 1.121 ± 0.036
2.023MetGly: 2.023 ± 0.054
0.527MetHis: 0.527 ± 0.024
2.357MetIle: 2.357 ± 0.057
2.272MetLys: 2.272 ± 0.047
2.781MetLeu: 2.781 ± 0.053
0.95MetMet: 0.95 ± 0.031
1.638MetAsn: 1.638 ± 0.043
1.098MetPro: 1.098 ± 0.04
1.108MetGln: 1.108 ± 0.038
1.158MetArg: 1.158 ± 0.03
1.689MetSer: 1.689 ± 0.041
1.933MetThr: 1.933 ± 0.043
1.889MetVal: 1.889 ± 0.047
0.197MetTrp: 0.197 ± 0.016
0.792MetTyr: 0.792 ± 0.026
0.0MetXaa: 0.0 ± 0.0
Asn
3.035AsnAla: 3.035 ± 0.056
0.277AsnCys: 0.277 ± 0.019
2.805AsnAsp: 2.805 ± 0.065
3.628AsnGlu: 3.628 ± 0.068
1.617AsnPhe: 1.617 ± 0.041
3.453AsnGly: 3.453 ± 0.063
1.158AsnHis: 1.158 ± 0.03
3.365AsnIle: 3.365 ± 0.058
2.533AsnLys: 2.533 ± 0.057
3.745AsnLeu: 3.745 ± 0.063
1.363AsnMet: 1.363 ± 0.038
2.111AsnAsn: 2.111 ± 0.06
2.191AsnPro: 2.191 ± 0.048
2.187AsnGln: 2.187 ± 0.052
2.136AsnArg: 2.136 ± 0.043
2.148AsnSer: 2.148 ± 0.045
2.395AsnThr: 2.395 ± 0.052
3.211AsnVal: 3.211 ± 0.057
0.541AsnTrp: 0.541 ± 0.023
1.56AsnTyr: 1.56 ± 0.043
0.0AsnXaa: 0.0 ± 0.0
Pro
2.446ProAla: 2.446 ± 0.049
0.174ProCys: 0.174 ± 0.014
2.592ProAsp: 2.592 ± 0.051
3.421ProGlu: 3.421 ± 0.062
1.942ProPhe: 1.942 ± 0.046
2.675ProGly: 2.675 ± 0.059
0.805ProHis: 0.805 ± 0.032
2.764ProIle: 2.764 ± 0.059
1.896ProLys: 1.896 ± 0.049
3.326ProLeu: 3.326 ± 0.056
0.878ProMet: 0.878 ± 0.029
1.598ProAsn: 1.598 ± 0.037
1.088ProPro: 1.088 ± 0.033
1.029ProGln: 1.029 ± 0.032
1.118ProArg: 1.118 ± 0.033
2.254ProSer: 2.254 ± 0.047
1.741ProThr: 1.741 ± 0.041
3.132ProVal: 3.132 ± 0.051
0.366ProTrp: 0.366 ± 0.021
1.461ProTyr: 1.461 ± 0.045
0.0ProXaa: 0.0 ± 0.0
Gln
2.878GlnAla: 2.878 ± 0.06
0.173GlnCys: 0.173 ± 0.014
2.105GlnAsp: 2.105 ± 0.048
3.215GlnGlu: 3.215 ± 0.061
1.516GlnPhe: 1.516 ± 0.036
2.263GlnGly: 2.263 ± 0.054
0.959GlnHis: 0.959 ± 0.029
2.635GlnIle: 2.635 ± 0.048
2.59GlnLys: 2.59 ± 0.055
4.042GlnLeu: 4.042 ± 0.081
1.322GlnMet: 1.322 ± 0.036
1.861GlnAsn: 1.861 ± 0.049
1.313GlnPro: 1.313 ± 0.04
2.106GlnGln: 2.106 ± 0.066
1.491GlnArg: 1.491 ± 0.042
2.352GlnSer: 2.352 ± 0.056
2.204GlnThr: 2.204 ± 0.051
2.377GlnVal: 2.377 ± 0.05
0.421GlnTrp: 0.421 ± 0.021
1.362GlnTyr: 1.362 ± 0.04
0.0GlnXaa: 0.0 ± 0.0
Arg
2.32ArgAla: 2.32 ± 0.051
0.261ArgCys: 0.261 ± 0.018
2.307ArgAsp: 2.307 ± 0.043
3.262ArgGlu: 3.262 ± 0.062
1.923ArgPhe: 1.923 ± 0.053
2.299ArgGly: 2.299 ± 0.05
0.93ArgHis: 0.93 ± 0.035
3.049ArgIle: 3.049 ± 0.065
3.107ArgLys: 3.107 ± 0.053
3.824ArgLeu: 3.824 ± 0.065
1.369ArgMet: 1.369 ± 0.036
2.045ArgAsn: 2.045 ± 0.043
1.283ArgPro: 1.283 ± 0.035
1.738ArgGln: 1.738 ± 0.04
1.877ArgArg: 1.877 ± 0.047
2.254ArgSer: 2.254 ± 0.05
2.136ArgThr: 2.136 ± 0.046
2.521ArgVal: 2.521 ± 0.05
0.385ArgTrp: 0.385 ± 0.02
1.511ArgTyr: 1.511 ± 0.038
0.0ArgXaa: 0.0 ± 0.0
Ser
4.04SerAla: 4.04 ± 0.07
0.363SerCys: 0.363 ± 0.02
3.458SerAsp: 3.458 ± 0.064
4.174SerGlu: 4.174 ± 0.068
2.98SerPhe: 2.98 ± 0.053
4.734SerGly: 4.734 ± 0.069
1.253SerHis: 1.253 ± 0.037
4.978SerIle: 4.978 ± 0.074
3.183SerLys: 3.183 ± 0.059
5.641SerLeu: 5.641 ± 0.083
1.871SerMet: 1.871 ± 0.038
2.47SerAsn: 2.47 ± 0.051
2.079SerPro: 2.079 ± 0.046
2.054SerGln: 2.054 ± 0.049
2.395SerArg: 2.395 ± 0.054
3.739SerSer: 3.739 ± 0.073
2.954SerThr: 2.954 ± 0.053
4.232SerVal: 4.232 ± 0.067
0.606SerTrp: 0.606 ± 0.027
2.142SerTyr: 2.142 ± 0.046
0.0SerXaa: 0.0 ± 0.0
Thr
4.439ThrAla: 4.439 ± 0.074
0.36ThrCys: 0.36 ± 0.019
3.371ThrAsp: 3.371 ± 0.063
3.71ThrGlu: 3.71 ± 0.064
2.71ThrPhe: 2.71 ± 0.056
4.552ThrGly: 4.552 ± 0.067
1.047ThrHis: 1.047 ± 0.039
4.597ThrIle: 4.597 ± 0.068
2.931ThrLys: 2.931 ± 0.056
4.95ThrLeu: 4.95 ± 0.078
1.433ThrMet: 1.433 ± 0.039
2.393ThrAsn: 2.393 ± 0.055
2.263ThrPro: 2.263 ± 0.045
1.454ThrGln: 1.454 ± 0.041
1.924ThrArg: 1.924 ± 0.045
3.341ThrSer: 3.341 ± 0.058
2.935ThrThr: 2.935 ± 0.053
4.314ThrVal: 4.314 ± 0.065
0.54ThrTrp: 0.54 ± 0.025
2.011ThrTyr: 2.011 ± 0.045
0.0ThrXaa: 0.0 ± 0.0
Val
4.747ValAla: 4.747 ± 0.078
0.545ValCys: 0.545 ± 0.026
4.0ValAsp: 4.0 ± 0.071
4.621ValGlu: 4.621 ± 0.074
3.126ValPhe: 3.126 ± 0.062
4.494ValGly: 4.494 ± 0.078
1.419ValHis: 1.419 ± 0.041
5.852ValIle: 5.852 ± 0.083
4.036ValLys: 4.036 ± 0.067
6.722ValLeu: 6.722 ± 0.09
2.156ValMet: 2.156 ± 0.049
3.194ValAsn: 3.194 ± 0.058
2.732ValPro: 2.732 ± 0.057
2.418ValGln: 2.418 ± 0.056
2.63ValArg: 2.63 ± 0.049
4.564ValSer: 4.564 ± 0.068
4.327ValThr: 4.327 ± 0.065
4.969ValVal: 4.969 ± 0.07
0.624ValTrp: 0.624 ± 0.023
2.305ValTyr: 2.305 ± 0.044
0.0ValXaa: 0.0 ± 0.0
Trp
0.613TrpAla: 0.613 ± 0.024
0.061TrpCys: 0.061 ± 0.008
0.586TrpAsp: 0.586 ± 0.029
0.698TrpGlu: 0.698 ± 0.026
0.501TrpPhe: 0.501 ± 0.023
0.655TrpGly: 0.655 ± 0.025
0.215TrpHis: 0.215 ± 0.014
0.796TrpIle: 0.796 ± 0.027
0.656TrpLys: 0.656 ± 0.022
1.13TrpLeu: 1.13 ± 0.035
0.352TrpMet: 0.352 ± 0.019
0.546TrpAsn: 0.546 ± 0.023
0.301TrpPro: 0.301 ± 0.021
0.424TrpGln: 0.424 ± 0.023
0.372TrpArg: 0.372 ± 0.018
0.54TrpSer: 0.54 ± 0.023
0.501TrpThr: 0.501 ± 0.021
0.663TrpVal: 0.663 ± 0.024
0.136TrpTrp: 0.136 ± 0.012
0.341TrpTyr: 0.341 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.201TyrAla: 2.201 ± 0.048
0.245TyrCys: 0.245 ± 0.016
2.253TyrAsp: 2.253 ± 0.047
2.429TyrGlu: 2.429 ± 0.045
1.74TyrPhe: 1.74 ± 0.04
2.625TyrGly: 2.625 ± 0.057
0.912TyrHis: 0.912 ± 0.03
2.437TyrIle: 2.437 ± 0.053
1.773TyrLys: 1.773 ± 0.043
3.281TyrLeu: 3.281 ± 0.068
0.904TyrMet: 0.904 ± 0.027
1.442TyrAsn: 1.442 ± 0.044
1.484TyrPro: 1.484 ± 0.036
1.65TyrGln: 1.65 ± 0.041
1.571TyrArg: 1.571 ± 0.041
1.86TyrSer: 1.86 ± 0.042
1.902TyrThr: 1.902 ± 0.046
2.291TyrVal: 2.291 ± 0.052
0.389TyrTrp: 0.389 ± 0.02
1.446TyrTyr: 1.446 ± 0.043
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3521 proteins (1011085 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski