Lactobacillus phage PLE2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Pleeduovirus; Lactobacillus virus PLE2

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B0Y2R8|A0A1B0Y2R8_9CAUD Head-tail joining protein OS=Lactobacillus phage PLE2 OX=1815511 GN=PLE2_8 PE=4 SV=1
MM1 pKa = 7.75ADD3 pKa = 3.35EE4 pKa = 4.73KK5 pKa = 11.35SEE7 pKa = 4.13EE8 pKa = 4.28EE9 pKa = 4.13PTLLDD14 pKa = 4.45LLKK17 pKa = 10.47QHH19 pKa = 6.48IRR21 pKa = 11.84LEE23 pKa = 4.32DD24 pKa = 3.91DD25 pKa = 3.68MDD27 pKa = 5.1PSMLQFYY34 pKa = 10.76LDD36 pKa = 3.69AADD39 pKa = 4.86KK40 pKa = 10.19YY41 pKa = 7.76VQRR44 pKa = 11.84KK45 pKa = 8.24VGHH48 pKa = 4.93SVKK51 pKa = 10.44YY52 pKa = 9.05LQLMVATVMNDD63 pKa = 2.73NRR65 pKa = 11.84SAGDD69 pKa = 3.65DD70 pKa = 3.29LAAALEE76 pKa = 4.0ALEE79 pKa = 4.81PIFYY83 pKa = 10.85LEE85 pKa = 4.66VRR87 pKa = 11.84TDD89 pKa = 4.06DD90 pKa = 4.87PDD92 pKa = 3.76SQSNEE97 pKa = 3.66PTQVDD102 pKa = 3.69SHH104 pKa = 5.66TVGTT108 pKa = 4.34

Molecular weight: 12.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B0Y2T1|A0A1B0Y2T1_9CAUD Uncharacterized protein OS=Lactobacillus phage PLE2 OX=1815511 GN=PLE2_46 PE=4 SV=1
MM1 pKa = 7.79SRR3 pKa = 11.84LLDD6 pKa = 4.12DD7 pKa = 4.91KK8 pKa = 11.35QLEE11 pKa = 4.61TYY13 pKa = 10.82KK14 pKa = 10.95NFVPGHH20 pKa = 5.07TAAEE24 pKa = 4.2IANMVHH30 pKa = 6.09EE31 pKa = 4.25NWGIQLTVQKK41 pKa = 10.43VHH43 pKa = 6.79ALNIRR48 pKa = 11.84NNIKK52 pKa = 10.27SGLYY56 pKa = 9.24QKK58 pKa = 10.97YY59 pKa = 9.6FGKK62 pKa = 10.45ADD64 pKa = 3.54PRR66 pKa = 11.84RR67 pKa = 11.84SSSHH71 pKa = 6.52HH72 pKa = 6.72DD73 pKa = 3.1LHH75 pKa = 8.37KK76 pKa = 10.87RR77 pKa = 11.84MAIGTVKK84 pKa = 10.62KK85 pKa = 10.76NEE87 pKa = 4.06TRR89 pKa = 11.84SKK91 pKa = 10.69DD92 pKa = 3.5RR93 pKa = 11.84PNRR96 pKa = 11.84APIVVVKK103 pKa = 10.23QSEE106 pKa = 4.55RR107 pKa = 11.84KK108 pKa = 8.27WKK110 pKa = 9.43PNHH113 pKa = 5.08RR114 pKa = 11.84RR115 pKa = 11.84IWEE118 pKa = 3.93EE119 pKa = 3.72AYY121 pKa = 10.43GPIPQGYY128 pKa = 7.69KK129 pKa = 9.61TVFLDD134 pKa = 4.03GNSLNFSITNLALVTDD150 pKa = 4.45AEE152 pKa = 4.2FLIMNEE158 pKa = 3.69KK159 pKa = 10.46HH160 pKa = 6.56LISSDD165 pKa = 3.43KK166 pKa = 10.49QVTRR170 pKa = 11.84SGIEE174 pKa = 3.8LARR177 pKa = 11.84LLSKK181 pKa = 8.29THH183 pKa = 5.3QIKK186 pKa = 10.05RR187 pKa = 11.84RR188 pKa = 11.84KK189 pKa = 9.31RR190 pKa = 11.84KK191 pKa = 9.35NEE193 pKa = 3.54RR194 pKa = 11.84SS195 pKa = 3.41

Molecular weight: 22.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

11370

43

1467

222.9

24.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.698 ± 0.471

0.281 ± 0.077

7.08 ± 0.32

5.154 ± 0.374

3.448 ± 0.183

6.271 ± 0.308

1.882 ± 0.179

6.526 ± 0.252

6.948 ± 0.418

8.461 ± 0.331

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.507 ± 0.18

5.418 ± 0.264

3.518 ± 0.221

4.609 ± 0.272

4.336 ± 0.45

6.526 ± 0.461

6.851 ± 0.46

6.588 ± 0.216

1.434 ± 0.106

3.465 ± 0.314
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski