Amino acid dipepetide frequency for Lactobacillus phage PLE2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.62AlaAla: 8.62 ± 1.352
0.088AlaCys: 0.088 ± 0.09
6.597AlaAsp: 6.597 ± 0.812
4.662AlaGlu: 4.662 ± 0.586
3.518AlaPhe: 3.518 ± 0.619
5.278AlaGly: 5.278 ± 0.908
1.495AlaHis: 1.495 ± 0.335
6.949AlaIle: 6.949 ± 0.974
6.421AlaLys: 6.421 ± 0.651
7.125AlaLeu: 7.125 ± 0.913
1.847AlaMet: 1.847 ± 0.443
5.629AlaAsn: 5.629 ± 0.831
2.199AlaPro: 2.199 ± 0.408
4.046AlaGln: 4.046 ± 0.714
2.551AlaArg: 2.551 ± 0.637
4.574AlaSer: 4.574 ± 0.56
5.893AlaThr: 5.893 ± 1.011
5.19AlaVal: 5.19 ± 1.03
0.88AlaTrp: 0.88 ± 0.332
3.43AlaTyr: 3.43 ± 0.602
0.0AlaXaa: 0.0 ± 0.0
Cys
0.352CysAla: 0.352 ± 0.174
0.0CysCys: 0.0 ± 0.0
0.088CysAsp: 0.088 ± 0.099
0.088CysGlu: 0.088 ± 0.095
0.176CysPhe: 0.176 ± 0.121
0.176CysGly: 0.176 ± 0.127
0.088CysHis: 0.088 ± 0.095
0.176CysIle: 0.176 ± 0.122
0.176CysLys: 0.176 ± 0.107
0.264CysLeu: 0.264 ± 0.163
0.0CysMet: 0.0 ± 0.0
0.0CysAsn: 0.0 ± 0.0
0.176CysPro: 0.176 ± 0.181
0.0CysGln: 0.0 ± 0.0
0.264CysArg: 0.264 ± 0.154
0.0CysSer: 0.0 ± 0.0
0.264CysThr: 0.264 ± 0.158
0.176CysVal: 0.176 ± 0.112
0.088CysTrp: 0.088 ± 0.075
0.264CysTyr: 0.264 ± 0.168
0.0CysXaa: 0.0 ± 0.0
Asp
6.685AspAla: 6.685 ± 0.757
0.352AspCys: 0.352 ± 0.185
4.926AspAsp: 4.926 ± 0.614
4.486AspGlu: 4.486 ± 0.741
2.463AspPhe: 2.463 ± 0.358
5.365AspGly: 5.365 ± 0.765
1.759AspHis: 1.759 ± 0.579
4.75AspIle: 4.75 ± 0.553
3.87AspLys: 3.87 ± 0.499
5.981AspLeu: 5.981 ± 0.746
1.583AspMet: 1.583 ± 0.375
3.782AspAsn: 3.782 ± 0.339
3.167AspPro: 3.167 ± 0.667
3.518AspGln: 3.518 ± 0.592
2.991AspArg: 2.991 ± 0.395
4.046AspSer: 4.046 ± 0.574
2.991AspThr: 2.991 ± 0.649
4.31AspVal: 4.31 ± 0.615
0.968AspTrp: 0.968 ± 0.259
2.815AspTyr: 2.815 ± 0.501
0.0AspXaa: 0.0 ± 0.0
Glu
3.694GluAla: 3.694 ± 0.521
0.0GluCys: 0.0 ± 0.0
3.079GluAsp: 3.079 ± 0.46
2.639GluGlu: 2.639 ± 0.695
2.199GluPhe: 2.199 ± 0.463
2.287GluGly: 2.287 ± 0.327
1.143GluHis: 1.143 ± 0.375
2.991GluIle: 2.991 ± 0.508
3.606GluLys: 3.606 ± 0.64
5.014GluLeu: 5.014 ± 0.641
1.143GluMet: 1.143 ± 0.386
2.991GluAsn: 2.991 ± 0.462
1.319GluPro: 1.319 ± 0.472
3.606GluGln: 3.606 ± 0.626
2.639GluArg: 2.639 ± 0.518
3.342GluSer: 3.342 ± 0.667
4.31GluThr: 4.31 ± 0.612
2.287GluVal: 2.287 ± 0.5
0.616GluTrp: 0.616 ± 0.271
2.551GluTyr: 2.551 ± 0.459
0.0GluXaa: 0.0 ± 0.0
Phe
2.727PheAla: 2.727 ± 0.445
0.088PheCys: 0.088 ± 0.09
3.167PheAsp: 3.167 ± 0.59
2.199PheGlu: 2.199 ± 0.477
1.671PhePhe: 1.671 ± 0.377
2.111PheGly: 2.111 ± 0.348
0.616PheHis: 0.616 ± 0.248
1.759PheIle: 1.759 ± 0.468
2.463PheLys: 2.463 ± 0.59
2.815PheLeu: 2.815 ± 0.544
0.968PheMet: 0.968 ± 0.267
2.199PheAsn: 2.199 ± 0.658
0.528PhePro: 0.528 ± 0.228
1.407PheGln: 1.407 ± 0.46
1.056PheArg: 1.056 ± 0.311
2.903PheSer: 2.903 ± 0.67
1.935PheThr: 1.935 ± 0.387
2.287PheVal: 2.287 ± 0.596
0.44PheTrp: 0.44 ± 0.167
1.143PheTyr: 1.143 ± 0.315
0.0PheXaa: 0.0 ± 0.0
Gly
4.486GlyAla: 4.486 ± 0.819
0.264GlyCys: 0.264 ± 0.168
3.958GlyAsp: 3.958 ± 0.724
3.958GlyGlu: 3.958 ± 0.606
2.551GlyPhe: 2.551 ± 0.366
4.75GlyGly: 4.75 ± 0.643
0.968GlyHis: 0.968 ± 0.283
5.102GlyIle: 5.102 ± 0.792
5.102GlyLys: 5.102 ± 0.756
4.75GlyLeu: 4.75 ± 0.52
1.671GlyMet: 1.671 ± 0.313
2.551GlyAsn: 2.551 ± 0.433
1.847GlyPro: 1.847 ± 0.505
2.727GlyGln: 2.727 ± 0.384
2.199GlyArg: 2.199 ± 0.53
2.639GlySer: 2.639 ± 0.451
4.662GlyThr: 4.662 ± 0.632
3.958GlyVal: 3.958 ± 0.621
1.407GlyTrp: 1.407 ± 0.311
3.167GlyTyr: 3.167 ± 0.573
0.0GlyXaa: 0.0 ± 0.0
His
1.143HisAla: 1.143 ± 0.333
0.088HisCys: 0.088 ± 0.1
1.847HisAsp: 1.847 ± 0.378
0.88HisGlu: 0.88 ± 0.318
0.792HisPhe: 0.792 ± 0.248
1.056HisGly: 1.056 ± 0.267
0.528HisHis: 0.528 ± 0.191
1.935HisIle: 1.935 ± 0.331
0.616HisLys: 0.616 ± 0.237
1.671HisLeu: 1.671 ± 0.352
0.264HisMet: 0.264 ± 0.142
0.968HisAsn: 0.968 ± 0.307
0.44HisPro: 0.44 ± 0.235
0.704HisGln: 0.704 ± 0.259
1.231HisArg: 1.231 ± 0.409
1.319HisSer: 1.319 ± 0.326
1.407HisThr: 1.407 ± 0.464
1.319HisVal: 1.319 ± 0.355
0.264HisTrp: 0.264 ± 0.188
0.352HisTyr: 0.352 ± 0.228
0.0HisXaa: 0.0 ± 0.0
Ile
6.421IleAla: 6.421 ± 0.779
0.088IleCys: 0.088 ± 0.08
4.486IleAsp: 4.486 ± 0.639
3.694IleGlu: 3.694 ± 0.658
1.583IlePhe: 1.583 ± 0.35
3.694IleGly: 3.694 ± 0.698
0.968IleHis: 0.968 ± 0.283
3.167IleIle: 3.167 ± 0.541
5.453IleLys: 5.453 ± 0.797
3.254IleLeu: 3.254 ± 0.82
1.935IleMet: 1.935 ± 0.495
3.167IleAsn: 3.167 ± 0.942
3.518IlePro: 3.518 ± 0.481
3.167IleGln: 3.167 ± 0.551
3.342IleArg: 3.342 ± 0.543
3.782IleSer: 3.782 ± 0.692
5.278IleThr: 5.278 ± 0.657
4.398IleVal: 4.398 ± 0.564
1.495IleTrp: 1.495 ± 0.316
2.375IleTyr: 2.375 ± 0.658
0.0IleXaa: 0.0 ± 0.0
Lys
4.926LysAla: 4.926 ± 0.621
0.088LysCys: 0.088 ± 0.075
5.102LysAsp: 5.102 ± 0.801
3.079LysGlu: 3.079 ± 0.524
1.847LysPhe: 1.847 ± 0.384
4.222LysGly: 4.222 ± 0.762
1.407LysHis: 1.407 ± 0.382
3.606LysIle: 3.606 ± 0.527
4.838LysLys: 4.838 ± 0.998
5.278LysLeu: 5.278 ± 0.663
1.847LysMet: 1.847 ± 0.408
2.903LysAsn: 2.903 ± 0.588
2.551LysPro: 2.551 ± 0.46
4.31LysGln: 4.31 ± 0.752
4.31LysArg: 4.31 ± 0.929
5.453LysSer: 5.453 ± 0.714
5.805LysThr: 5.805 ± 0.537
5.102LysVal: 5.102 ± 0.67
0.792LysTrp: 0.792 ± 0.287
2.023LysTyr: 2.023 ± 0.458
0.0LysXaa: 0.0 ± 0.0
Leu
8.532LeuAla: 8.532 ± 1.205
0.44LeuCys: 0.44 ± 0.254
5.014LeuAsp: 5.014 ± 0.576
5.365LeuGlu: 5.365 ± 0.801
3.167LeuPhe: 3.167 ± 0.448
4.486LeuGly: 4.486 ± 0.822
1.231LeuHis: 1.231 ± 0.416
4.926LeuIle: 4.926 ± 0.985
7.037LeuLys: 7.037 ± 0.696
7.125LeuLeu: 7.125 ± 0.889
1.935LeuMet: 1.935 ± 0.416
4.838LeuAsn: 4.838 ± 0.864
2.815LeuPro: 2.815 ± 0.454
3.254LeuGln: 3.254 ± 0.57
3.87LeuArg: 3.87 ± 0.674
6.949LeuSer: 6.949 ± 0.837
5.014LeuThr: 5.014 ± 0.832
5.453LeuVal: 5.453 ± 0.677
0.968LeuTrp: 0.968 ± 0.295
2.199LeuTyr: 2.199 ± 0.463
0.0LeuXaa: 0.0 ± 0.0
Met
2.903MetAla: 2.903 ± 0.56
0.0MetCys: 0.0 ± 0.0
1.671MetAsp: 1.671 ± 0.391
0.88MetGlu: 0.88 ± 0.281
1.143MetPhe: 1.143 ± 0.267
0.968MetGly: 0.968 ± 0.318
0.44MetHis: 0.44 ± 0.222
0.792MetIle: 0.792 ± 0.286
2.199MetLys: 2.199 ± 0.557
1.407MetLeu: 1.407 ± 0.374
0.616MetMet: 0.616 ± 0.307
2.815MetAsn: 2.815 ± 0.493
1.231MetPro: 1.231 ± 0.377
0.616MetGln: 0.616 ± 0.225
0.88MetArg: 0.88 ± 0.283
2.199MetSer: 2.199 ± 0.433
1.935MetThr: 1.935 ± 0.411
1.583MetVal: 1.583 ± 0.387
0.176MetTrp: 0.176 ± 0.108
0.616MetTyr: 0.616 ± 0.185
0.0MetXaa: 0.0 ± 0.0
Asn
5.102AsnAla: 5.102 ± 1.076
0.0AsnCys: 0.0 ± 0.0
3.87AsnAsp: 3.87 ± 0.481
2.463AsnGlu: 2.463 ± 0.558
1.671AsnPhe: 1.671 ± 0.301
4.75AsnGly: 4.75 ± 0.536
1.231AsnHis: 1.231 ± 0.294
3.167AsnIle: 3.167 ± 0.507
3.518AsnLys: 3.518 ± 0.642
4.134AsnLeu: 4.134 ± 0.883
0.968AsnMet: 0.968 ± 0.324
3.167AsnAsn: 3.167 ± 0.493
2.199AsnPro: 2.199 ± 0.452
2.463AsnGln: 2.463 ± 0.413
2.551AsnArg: 2.551 ± 0.484
3.43AsnSer: 3.43 ± 0.517
3.518AsnThr: 3.518 ± 0.606
3.43AsnVal: 3.43 ± 0.64
0.792AsnTrp: 0.792 ± 0.279
1.759AsnTyr: 1.759 ± 0.424
0.0AsnXaa: 0.0 ± 0.0
Pro
2.375ProAla: 2.375 ± 0.461
0.0ProCys: 0.0 ± 0.0
2.551ProAsp: 2.551 ± 0.486
2.727ProGlu: 2.727 ± 0.51
1.231ProPhe: 1.231 ± 0.459
2.023ProGly: 2.023 ± 0.512
0.616ProHis: 0.616 ± 0.305
2.375ProIle: 2.375 ± 0.426
1.935ProLys: 1.935 ± 0.388
2.375ProLeu: 2.375 ± 0.557
0.792ProMet: 0.792 ± 0.241
2.287ProAsn: 2.287 ± 0.509
0.792ProPro: 0.792 ± 0.298
1.935ProGln: 1.935 ± 0.371
1.143ProArg: 1.143 ± 0.289
2.727ProSer: 2.727 ± 0.426
2.551ProThr: 2.551 ± 0.433
2.903ProVal: 2.903 ± 0.477
0.352ProTrp: 0.352 ± 0.177
1.495ProTyr: 1.495 ± 0.278
0.0ProXaa: 0.0 ± 0.0
Gln
3.694GlnAla: 3.694 ± 0.576
0.0GlnCys: 0.0 ± 0.0
1.847GlnAsp: 1.847 ± 0.542
2.639GlnGlu: 2.639 ± 0.465
1.407GlnPhe: 1.407 ± 0.328
2.991GlnGly: 2.991 ± 0.456
0.616GlnHis: 0.616 ± 0.287
3.43GlnIle: 3.43 ± 0.587
3.518GlnLys: 3.518 ± 0.634
5.102GlnLeu: 5.102 ± 0.575
1.495GlnMet: 1.495 ± 0.512
1.847GlnAsn: 1.847 ± 0.352
1.495GlnPro: 1.495 ± 0.287
1.847GlnGln: 1.847 ± 0.436
1.847GlnArg: 1.847 ± 0.463
3.254GlnSer: 3.254 ± 0.47
4.222GlnThr: 4.222 ± 0.896
2.903GlnVal: 2.903 ± 0.509
0.968GlnTrp: 0.968 ± 0.29
0.968GlnTyr: 0.968 ± 0.224
0.0GlnXaa: 0.0 ± 0.0
Arg
2.815ArgAla: 2.815 ± 0.355
0.176ArgCys: 0.176 ± 0.137
3.606ArgAsp: 3.606 ± 0.678
1.847ArgGlu: 1.847 ± 0.391
0.968ArgPhe: 0.968 ± 0.255
2.199ArgGly: 2.199 ± 0.396
1.143ArgHis: 1.143 ± 0.405
2.815ArgIle: 2.815 ± 0.598
2.463ArgLys: 2.463 ± 0.672
5.893ArgLeu: 5.893 ± 1.015
0.88ArgMet: 0.88 ± 0.265
2.199ArgAsn: 2.199 ± 0.476
1.407ArgPro: 1.407 ± 0.385
1.495ArgGln: 1.495 ± 0.437
3.254ArgArg: 3.254 ± 0.627
2.815ArgSer: 2.815 ± 0.558
2.903ArgThr: 2.903 ± 0.536
2.463ArgVal: 2.463 ± 0.547
0.616ArgTrp: 0.616 ± 0.247
1.407ArgTyr: 1.407 ± 0.329
0.0ArgXaa: 0.0 ± 0.0
Ser
5.717SerAla: 5.717 ± 0.881
0.264SerCys: 0.264 ± 0.145
4.486SerAsp: 4.486 ± 0.599
3.167SerGlu: 3.167 ± 0.498
2.815SerPhe: 2.815 ± 0.611
5.102SerGly: 5.102 ± 0.867
1.056SerHis: 1.056 ± 0.362
4.838SerIle: 4.838 ± 0.607
4.31SerLys: 4.31 ± 0.441
5.014SerLeu: 5.014 ± 0.664
2.199SerMet: 2.199 ± 0.427
3.254SerAsn: 3.254 ± 0.62
2.551SerPro: 2.551 ± 0.486
2.463SerGln: 2.463 ± 0.586
2.111SerArg: 2.111 ± 0.367
4.486SerSer: 4.486 ± 0.949
3.87SerThr: 3.87 ± 0.891
4.486SerVal: 4.486 ± 0.672
1.056SerTrp: 1.056 ± 0.292
2.023SerTyr: 2.023 ± 0.435
0.0SerXaa: 0.0 ± 0.0
Thr
6.685ThrAla: 6.685 ± 0.926
0.176ThrCys: 0.176 ± 0.114
5.453ThrAsp: 5.453 ± 0.935
2.199ThrGlu: 2.199 ± 0.396
2.551ThrPhe: 2.551 ± 0.499
3.958ThrGly: 3.958 ± 0.96
1.495ThrHis: 1.495 ± 0.459
4.838ThrIle: 4.838 ± 0.713
3.606ThrLys: 3.606 ± 0.534
6.333ThrLeu: 6.333 ± 0.726
2.111ThrMet: 2.111 ± 0.395
2.727ThrAsn: 2.727 ± 0.475
2.727ThrPro: 2.727 ± 0.606
3.518ThrGln: 3.518 ± 0.606
2.199ThrArg: 2.199 ± 0.54
4.31ThrSer: 4.31 ± 0.805
4.222ThrThr: 4.222 ± 0.781
6.333ThrVal: 6.333 ± 0.932
1.056ThrTrp: 1.056 ± 0.228
2.023ThrTyr: 2.023 ± 0.334
0.0ThrXaa: 0.0 ± 0.0
Val
6.333ValAla: 6.333 ± 0.826
0.44ValCys: 0.44 ± 0.19
5.717ValAsp: 5.717 ± 0.606
2.815ValGlu: 2.815 ± 0.453
1.319ValPhe: 1.319 ± 0.303
3.958ValGly: 3.958 ± 0.647
1.056ValHis: 1.056 ± 0.293
5.014ValIle: 5.014 ± 0.736
4.662ValLys: 4.662 ± 0.73
5.102ValLeu: 5.102 ± 0.699
1.583ValMet: 1.583 ± 0.382
3.606ValAsn: 3.606 ± 0.462
2.463ValPro: 2.463 ± 0.579
2.551ValGln: 2.551 ± 0.624
2.287ValArg: 2.287 ± 0.487
4.662ValSer: 4.662 ± 0.727
5.014ValThr: 5.014 ± 0.584
4.31ValVal: 4.31 ± 0.742
0.704ValTrp: 0.704 ± 0.306
2.287ValTyr: 2.287 ± 0.453
0.0ValXaa: 0.0 ± 0.0
Trp
0.792TrpAla: 0.792 ± 0.307
0.176TrpCys: 0.176 ± 0.122
0.968TrpAsp: 0.968 ± 0.28
0.44TrpGlu: 0.44 ± 0.21
0.176TrpPhe: 0.176 ± 0.114
1.319TrpGly: 1.319 ± 0.376
0.44TrpHis: 0.44 ± 0.197
0.704TrpIle: 0.704 ± 0.223
0.968TrpLys: 0.968 ± 0.326
2.199TrpLeu: 2.199 ± 0.459
0.088TrpMet: 0.088 ± 0.089
1.495TrpAsn: 1.495 ± 0.757
0.44TrpPro: 0.44 ± 0.192
0.528TrpGln: 0.528 ± 0.237
0.88TrpArg: 0.88 ± 0.361
0.704TrpSer: 0.704 ± 0.264
0.88TrpThr: 0.88 ± 0.294
0.616TrpVal: 0.616 ± 0.291
0.264TrpTrp: 0.264 ± 0.172
0.264TrpTyr: 0.264 ± 0.132
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.991TyrAla: 2.991 ± 0.407
0.088TyrCys: 0.088 ± 0.075
2.375TyrAsp: 2.375 ± 0.621
1.319TyrGlu: 1.319 ± 0.362
1.231TyrPhe: 1.231 ± 0.333
2.023TyrGly: 2.023 ± 0.521
0.528TyrHis: 0.528 ± 0.257
1.935TyrIle: 1.935 ± 0.473
2.727TyrLys: 2.727 ± 0.554
3.782TyrLeu: 3.782 ± 0.587
1.056TyrMet: 1.056 ± 0.251
1.759TyrAsn: 1.759 ± 0.475
1.319TyrPro: 1.319 ± 0.318
1.935TyrGln: 1.935 ± 0.362
1.759TyrArg: 1.759 ± 0.397
1.671TyrSer: 1.671 ± 0.374
1.847TyrThr: 1.847 ± 0.498
2.375TyrVal: 2.375 ± 0.504
0.44TyrTrp: 0.44 ± 0.233
1.495TyrTyr: 1.495 ± 0.335
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 51 proteins (11370 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski