Ekpoma virus 2
Average proteome isoelectric point is 7.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C5BZR0|A0A0C5BZR0_9RHAB U3 protein OS=Ekpoma virus 2 OX=1987021 PE=4 SV=1
MM1 pKa = 7.53 NKK3 pKa = 9.55 PDD5 pKa = 4.53 FSKK8 pKa = 10.88 LYY10 pKa = 10.47 QNYY13 pKa = 10.34 SMEE16 pKa = 4.25 KK17 pKa = 10.26 LRR19 pKa = 11.84 DD20 pKa = 3.61 SLRR23 pKa = 11.84 DD24 pKa = 3.32 MALQPNDD31 pKa = 4.35 EE32 pKa = 4.31 VPEE35 pKa = 4.33 DD36 pKa = 4.84 PIHH39 pKa = 6.56 NNPSTSTGYY48 pKa = 8.61 TNKK51 pKa = 10.08 NPLEE55 pKa = 4.22 SSKK58 pKa = 9.89 LTPSYY63 pKa = 10.81 DD64 pKa = 3.37 WEE66 pKa = 4.48 SSISPDD72 pKa = 3.39 TSLTQQSKK80 pKa = 10.8 LDD82 pKa = 4.36 PIKK85 pKa = 10.48 EE86 pKa = 4.23 VFPMASLSDD95 pKa = 3.55 ILRR98 pKa = 11.84 ALALFNIHH106 pKa = 6.29 EE107 pKa = 4.44 SMDD110 pKa = 3.67 FEE112 pKa = 4.58 ISCEE116 pKa = 3.84 KK117 pKa = 10.06 LTFSIFPINRR127 pKa = 11.84 SVTQNISSTPEE138 pKa = 3.49 HH139 pKa = 6.59 GKK141 pKa = 8.31 TYY143 pKa = 7.13 PTLRR147 pKa = 11.84 EE148 pKa = 3.79 VLEE151 pKa = 4.28 RR152 pKa = 11.84 GIIVHH157 pKa = 6.97 KK158 pKa = 9.67 LPSMKK163 pKa = 9.98 PVTIHH168 pKa = 6.55 AWTKK172 pKa = 10.52 GINLEE177 pKa = 4.69 KK178 pKa = 10.04 IDD180 pKa = 4.32 DD181 pKa = 3.99 LMYY184 pKa = 10.91 ADD186 pKa = 5.83 DD187 pKa = 5.07 MDD189 pKa = 5.21 DD190 pKa = 3.79 VEE192 pKa = 5.98 CIAKK196 pKa = 9.15 TIIKK200 pKa = 10.13 ASRR203 pKa = 11.84 LTRR206 pKa = 11.84 SLTHH210 pKa = 6.76 EE211 pKa = 4.5 GAKK214 pKa = 10.14 HH215 pKa = 5.42 CAA217 pKa = 3.33
Molecular weight: 24.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.367
IPC2_protein 5.334
IPC_protein 5.308
Toseland 5.512
ProMoST 5.55
Dawson 5.436
Bjellqvist 5.499
Wikipedia 5.372
Rodwell 5.385
Grimsley 5.563
Solomon 5.436
Lehninger 5.41
Nozaki 5.626
DTASelect 5.804
Thurlkill 5.639
EMBOSS 5.601
Sillero 5.703
Patrickios 4.202
IPC_peptide 5.448
IPC2_peptide 5.715
IPC2.peptide.svr19 5.694
Protein with the highest isoelectric point:
>tr|A0A0C5C384|A0A0C5C384_9RHAB Nucleocapsid protein OS=Ekpoma virus 2 OX=1987021 PE=4 SV=1
MM1 pKa = 7.63 KK2 pKa = 9.21 KK3 pKa = 6.78 TTEE6 pKa = 3.82 NMGNSLSSHH15 pKa = 5.58 LEE17 pKa = 3.81 KK18 pKa = 10.93 FKK20 pKa = 9.12 TTIIWVIGGIGVCIVGCLLIKK41 pKa = 9.82 CTSCLHH47 pKa = 7.45 DD48 pKa = 5.86 IILCCASSKK57 pKa = 9.93 TSSPANKK64 pKa = 9.5 QMPTIYY70 pKa = 10.37 QKK72 pKa = 10.75 FKK74 pKa = 8.89 KK75 pKa = 9.72 QRR77 pKa = 11.84 RR78 pKa = 11.84 KK79 pKa = 10.4 KK80 pKa = 9.21 MKK82 pKa = 10.63 NKK84 pKa = 7.54 ITKK87 pKa = 9.99 LARR90 pKa = 11.84 TKK92 pKa = 10.52 AANKK96 pKa = 9.24 TSLKK100 pKa = 10.05 RR101 pKa = 11.84 IKK103 pKa = 10.29 PSTRR107 pKa = 11.84 SSLPDD112 pKa = 3.24 KK113 pKa = 10.8 RR114 pKa = 11.84 VGEE117 pKa = 4.39 LIGKK121 pKa = 9.23 YY122 pKa = 8.65 EE123 pKa = 4.06 AGG125 pKa = 3.53
Molecular weight: 13.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.154
IPC2_protein 9.472
IPC_protein 9.385
Toseland 10.57
ProMoST 9.999
Dawson 10.643
Bjellqvist 10.218
Wikipedia 10.716
Rodwell 11.403
Grimsley 10.657
Solomon 10.657
Lehninger 10.657
Nozaki 10.57
DTASelect 10.189
Thurlkill 10.54
EMBOSS 10.935
Sillero 10.555
Patrickios 11.14
IPC_peptide 10.672
IPC2_peptide 8.931
IPC2.peptide.svr19 8.403
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
4091
125
2120
511.4
58.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.74 ± 0.295
2.151 ± 0.28
5.475 ± 0.409
5.353 ± 0.253
3.667 ± 0.378
5.353 ± 0.278
2.982 ± 0.333
8.726 ± 0.436
7.162 ± 1.035
10.364 ± 0.942
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.64 ± 0.342
5.5 ± 0.369
5.109 ± 0.317
2.909 ± 0.181
4.351 ± 0.324
8.409 ± 0.587
5.989 ± 0.92
4.571 ± 0.247
1.76 ± 0.278
3.789 ± 0.297
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here