Streptococcus phage Javan623

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6ALJ4|A0A4D6ALJ4_9CAUD Uncharacterized protein OS=Streptococcus phage Javan623 OX=2548285 GN=Javan623_0033 PE=4 SV=1
MM1 pKa = 7.98KK2 pKa = 9.64YY3 pKa = 9.24TIEE6 pKa = 4.34LPYY9 pKa = 10.57HH10 pKa = 6.57SGPEE14 pKa = 4.09YY15 pKa = 11.05GNARR19 pKa = 11.84YY20 pKa = 7.51GTLEE24 pKa = 3.79EE25 pKa = 4.68LKK27 pKa = 10.72EE28 pKa = 3.82KK29 pKa = 10.87LLFKK33 pKa = 10.69RR34 pKa = 11.84IVEE37 pKa = 3.98WDD39 pKa = 3.7EE40 pKa = 3.82KK41 pKa = 11.15HH42 pKa = 5.98LTLEE46 pKa = 5.0DD47 pKa = 3.64GTEE50 pKa = 3.96VTIEE54 pKa = 4.15LSEE57 pKa = 4.33SDD59 pKa = 3.58CCAYY63 pKa = 10.52AGGEE67 pKa = 4.12FKK69 pKa = 10.57DD70 pKa = 3.76VKK72 pKa = 10.49LDD74 pKa = 3.64AVITDD79 pKa = 3.55VEE81 pKa = 4.15IGEE84 pKa = 4.44PVSDD88 pKa = 4.33DD89 pKa = 3.73SDD91 pKa = 3.42WVIRR95 pKa = 11.84KK96 pKa = 6.33TNKK99 pKa = 7.3VTIYY103 pKa = 10.6HH104 pKa = 5.63NQNPIAQADD113 pKa = 4.05CEE115 pKa = 4.37AEE117 pKa = 4.15HH118 pKa = 6.37NGYY121 pKa = 9.85YY122 pKa = 10.37YY123 pKa = 10.92SVGSLVIGDD132 pKa = 2.96IHH134 pKa = 6.9FPVVEE139 pKa = 4.09AA140 pKa = 4.75

Molecular weight: 15.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AKK1|A0A4D6AKK1_9CAUD Uncharacterized protein OS=Streptococcus phage Javan623 OX=2548285 GN=Javan623_0006 PE=4 SV=1
MM1 pKa = 7.2KK2 pKa = 10.02QVQPIRR8 pKa = 11.84DD9 pKa = 3.58RR10 pKa = 11.84DD11 pKa = 3.98DD12 pKa = 3.04IARR15 pKa = 11.84MKK17 pKa = 10.78DD18 pKa = 2.8ILRR21 pKa = 11.84PNIRR25 pKa = 11.84NYY27 pKa = 9.89TLFIVGLNCGLRR39 pKa = 11.84ISDD42 pKa = 3.45ILTLKK47 pKa = 10.26VRR49 pKa = 11.84DD50 pKa = 3.65VLGEE54 pKa = 3.99HH55 pKa = 7.06IEE57 pKa = 4.08IKK59 pKa = 10.07EE60 pKa = 4.1KK61 pKa = 9.99KK62 pKa = 7.63TGKK65 pKa = 8.17TRR67 pKa = 11.84MFLINDD73 pKa = 3.36QMKK76 pKa = 10.27RR77 pKa = 11.84ALTKK81 pKa = 10.41YY82 pKa = 10.05IKK84 pKa = 9.01QTGLKK89 pKa = 9.38EE90 pKa = 3.74YY91 pKa = 10.58DD92 pKa = 3.36YY93 pKa = 11.22LFPSNRR99 pKa = 11.84KK100 pKa = 6.47TKK102 pKa = 9.66EE103 pKa = 3.73GKK105 pKa = 8.84IQPISRR111 pKa = 11.84IQAYY115 pKa = 10.31RR116 pKa = 11.84ILNKK120 pKa = 9.81AAKK123 pKa = 9.4EE124 pKa = 3.85AGLVKK129 pKa = 10.36IGTHH133 pKa = 5.2TMRR136 pKa = 11.84KK137 pKa = 6.85TYY139 pKa = 10.15GYY141 pKa = 10.4HH142 pKa = 6.87FYY144 pKa = 10.78KK145 pKa = 10.27QYY147 pKa = 11.24KK148 pKa = 9.19SVAEE152 pKa = 3.75LMEE155 pKa = 4.47IFNHH159 pKa = 6.0SSPDD163 pKa = 2.78ITLIYY168 pKa = 10.17IGINQDD174 pKa = 3.27EE175 pKa = 4.86KK176 pKa = 10.81DD177 pKa = 3.17KK178 pKa = 11.38KK179 pKa = 10.22MRR181 pKa = 11.84GFGLL185 pKa = 3.63

Molecular weight: 21.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

11435

38

1627

207.9

23.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.393 ± 0.46

0.385 ± 0.087

6.113 ± 0.318

7.162 ± 0.486

4.049 ± 0.284

6.13 ± 0.431

1.251 ± 0.135

7.643 ± 0.355

8.981 ± 0.573

8.203 ± 0.36

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.239 ± 0.16

6.384 ± 0.256

2.816 ± 0.205

3.857 ± 0.245

3.516 ± 0.264

6.707 ± 0.452

7.084 ± 0.502

5.903 ± 0.262

1.207 ± 0.099

3.979 ± 0.342
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski