Amino acid dipepetide frequency for Streptococcus phage Javan623

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.186AlaAla: 2.186 ± 0.465
0.262AlaCys: 0.262 ± 0.158
3.848AlaAsp: 3.848 ± 0.512
5.073AlaGlu: 5.073 ± 0.559
2.711AlaPhe: 2.711 ± 0.478
3.673AlaGly: 3.673 ± 0.592
0.525AlaHis: 0.525 ± 0.148
4.46AlaIle: 4.46 ± 0.562
6.122AlaLys: 6.122 ± 1.227
5.248AlaLeu: 5.248 ± 1.111
2.186AlaMet: 2.186 ± 0.455
4.898AlaAsn: 4.898 ± 0.705
2.099AlaPro: 2.099 ± 0.541
2.361AlaGln: 2.361 ± 0.42
2.186AlaArg: 2.186 ± 0.385
5.073AlaSer: 5.073 ± 0.745
4.373AlaThr: 4.373 ± 0.893
2.886AlaVal: 2.886 ± 0.498
0.7AlaTrp: 0.7 ± 0.319
3.061AlaTyr: 3.061 ± 0.462
0.0AlaXaa: 0.0 ± 0.0
Cys
0.262CysAla: 0.262 ± 0.134
0.087CysCys: 0.087 ± 0.086
0.087CysAsp: 0.087 ± 0.093
0.262CysGlu: 0.262 ± 0.138
0.0CysPhe: 0.0 ± 0.0
0.262CysGly: 0.262 ± 0.166
0.0CysHis: 0.0 ± 0.0
0.175CysIle: 0.175 ± 0.133
0.437CysLys: 0.437 ± 0.21
0.525CysLeu: 0.525 ± 0.236
0.087CysMet: 0.087 ± 0.099
0.262CysAsn: 0.262 ± 0.145
0.087CysPro: 0.087 ± 0.086
0.087CysGln: 0.087 ± 0.087
0.087CysArg: 0.087 ± 0.063
0.087CysSer: 0.087 ± 0.087
0.087CysThr: 0.087 ± 0.087
0.437CysVal: 0.437 ± 0.206
0.087CysTrp: 0.087 ± 0.086
0.437CysTyr: 0.437 ± 0.257
0.0CysXaa: 0.0 ± 0.0
Asp
2.711AspAla: 2.711 ± 0.645
0.175AspCys: 0.175 ± 0.172
3.936AspAsp: 3.936 ± 0.795
4.548AspGlu: 4.548 ± 0.561
3.586AspPhe: 3.586 ± 0.626
5.51AspGly: 5.51 ± 0.946
0.612AspHis: 0.612 ± 0.207
4.198AspIle: 4.198 ± 1.154
5.685AspLys: 5.685 ± 0.689
5.335AspLeu: 5.335 ± 0.71
1.574AspMet: 1.574 ± 0.328
3.236AspAsn: 3.236 ± 0.41
1.574AspPro: 1.574 ± 0.347
1.224AspGln: 1.224 ± 0.3
2.274AspArg: 2.274 ± 0.444
4.373AspSer: 4.373 ± 0.657
3.411AspThr: 3.411 ± 0.646
2.799AspVal: 2.799 ± 0.462
0.962AspTrp: 0.962 ± 0.274
3.411AspTyr: 3.411 ± 0.592
0.0AspXaa: 0.0 ± 0.0
Glu
4.285GluAla: 4.285 ± 0.746
0.262GluCys: 0.262 ± 0.177
3.586GluAsp: 3.586 ± 0.556
4.898GluGlu: 4.898 ± 0.763
3.323GluPhe: 3.323 ± 0.637
2.711GluGly: 2.711 ± 0.457
0.875GluHis: 0.875 ± 0.307
7.959GluIle: 7.959 ± 1.03
6.384GluLys: 6.384 ± 1.158
7.696GluLeu: 7.696 ± 1.066
1.924GluMet: 1.924 ± 0.468
4.985GluAsn: 4.985 ± 0.797
1.312GluPro: 1.312 ± 0.335
2.711GluGln: 2.711 ± 0.455
2.536GluArg: 2.536 ± 0.487
3.848GluSer: 3.848 ± 0.731
4.548GluThr: 4.548 ± 0.776
5.16GluVal: 5.16 ± 0.683
0.787GluTrp: 0.787 ± 0.272
1.837GluTyr: 1.837 ± 0.411
0.0GluXaa: 0.0 ± 0.0
Phe
2.449PheAla: 2.449 ± 0.491
0.0PheCys: 0.0 ± 0.0
2.799PheAsp: 2.799 ± 0.588
2.536PheGlu: 2.536 ± 0.556
1.399PhePhe: 1.399 ± 0.44
2.624PheGly: 2.624 ± 0.481
0.35PheHis: 0.35 ± 0.155
4.285PheIle: 4.285 ± 0.617
4.985PheLys: 4.985 ± 0.727
2.799PheLeu: 2.799 ± 0.611
1.224PheMet: 1.224 ± 0.361
2.536PheAsn: 2.536 ± 0.383
0.787PhePro: 0.787 ± 0.349
0.612PheGln: 0.612 ± 0.208
1.487PheArg: 1.487 ± 0.415
3.323PheSer: 3.323 ± 0.546
1.924PheThr: 1.924 ± 0.34
1.837PheVal: 1.837 ± 0.433
0.787PheTrp: 0.787 ± 0.315
1.749PheTyr: 1.749 ± 0.417
0.0PheXaa: 0.0 ± 0.0
Gly
3.673GlyAla: 3.673 ± 0.67
0.175GlyCys: 0.175 ± 0.129
3.586GlyAsp: 3.586 ± 0.545
2.711GlyGlu: 2.711 ± 0.637
3.411GlyPhe: 3.411 ± 0.668
4.985GlyGly: 4.985 ± 0.998
1.05GlyHis: 1.05 ± 0.289
4.81GlyIle: 4.81 ± 0.667
4.46GlyLys: 4.46 ± 0.494
6.035GlyLeu: 6.035 ± 1.1
0.7GlyMet: 0.7 ± 0.212
3.061GlyAsn: 3.061 ± 0.466
1.137GlyPro: 1.137 ± 0.381
2.624GlyGln: 2.624 ± 0.454
2.099GlyArg: 2.099 ± 0.474
4.111GlySer: 4.111 ± 0.991
5.947GlyThr: 5.947 ± 0.94
3.761GlyVal: 3.761 ± 0.519
1.05GlyTrp: 1.05 ± 0.219
1.837GlyTyr: 1.837 ± 0.381
0.0GlyXaa: 0.0 ± 0.0
His
0.525HisAla: 0.525 ± 0.186
0.087HisCys: 0.087 ± 0.086
0.962HisAsp: 0.962 ± 0.261
0.7HisGlu: 0.7 ± 0.242
0.525HisPhe: 0.525 ± 0.206
0.7HisGly: 0.7 ± 0.316
0.35HisHis: 0.35 ± 0.169
1.05HisIle: 1.05 ± 0.295
0.962HisLys: 0.962 ± 0.241
0.962HisLeu: 0.962 ± 0.302
0.087HisMet: 0.087 ± 0.091
0.787HisAsn: 0.787 ± 0.282
0.525HisPro: 0.525 ± 0.243
0.612HisGln: 0.612 ± 0.2
0.262HisArg: 0.262 ± 0.136
0.875HisSer: 0.875 ± 0.26
0.787HisThr: 0.787 ± 0.279
1.137HisVal: 1.137 ± 0.393
0.0HisTrp: 0.0 ± 0.0
0.612HisTyr: 0.612 ± 0.189
0.0HisXaa: 0.0 ± 0.0
Ile
6.21IleAla: 6.21 ± 0.754
0.612IleCys: 0.612 ± 0.188
5.685IleAsp: 5.685 ± 0.696
7.259IleGlu: 7.259 ± 0.664
2.274IlePhe: 2.274 ± 0.589
4.198IleGly: 4.198 ± 0.636
1.224IleHis: 1.224 ± 0.244
4.023IleIle: 4.023 ± 0.694
7.084IleLys: 7.084 ± 1.022
5.248IleLeu: 5.248 ± 0.664
1.137IleMet: 1.137 ± 0.362
5.51IleAsn: 5.51 ± 0.8
3.149IlePro: 3.149 ± 0.55
3.061IleGln: 3.061 ± 0.426
2.449IleArg: 2.449 ± 0.399
5.335IleSer: 5.335 ± 0.928
5.073IleThr: 5.073 ± 0.533
3.761IleVal: 3.761 ± 0.73
0.612IleTrp: 0.612 ± 0.201
2.536IleTyr: 2.536 ± 0.525
0.0IleXaa: 0.0 ± 0.0
Lys
6.122LysAla: 6.122 ± 1.261
0.087LysCys: 0.087 ± 0.087
5.772LysAsp: 5.772 ± 0.759
6.909LysGlu: 6.909 ± 1.194
2.711LysPhe: 2.711 ± 0.59
4.373LysGly: 4.373 ± 0.57
0.612LysHis: 0.612 ± 0.23
6.384LysIle: 6.384 ± 0.759
8.833LysLys: 8.833 ± 1.256
7.959LysLeu: 7.959 ± 1.332
1.924LysMet: 1.924 ± 0.381
6.297LysAsn: 6.297 ± 0.809
2.449LysPro: 2.449 ± 0.54
4.285LysGln: 4.285 ± 0.938
4.285LysArg: 4.285 ± 0.562
4.985LysSer: 4.985 ± 0.622
6.734LysThr: 6.734 ± 0.805
4.635LysVal: 4.635 ± 0.726
0.962LysTrp: 0.962 ± 0.366
3.498LysTyr: 3.498 ± 0.539
0.0LysXaa: 0.0 ± 0.0
Leu
5.335LeuAla: 5.335 ± 0.84
0.35LeuCys: 0.35 ± 0.181
4.285LeuAsp: 4.285 ± 0.556
6.384LeuGlu: 6.384 ± 0.901
3.673LeuPhe: 3.673 ± 0.817
4.81LeuGly: 4.81 ± 0.721
0.875LeuHis: 0.875 ± 0.327
6.122LeuIle: 6.122 ± 0.551
6.559LeuLys: 6.559 ± 0.777
6.472LeuLeu: 6.472 ± 0.643
1.749LeuMet: 1.749 ± 0.444
5.772LeuAsn: 5.772 ± 0.797
2.711LeuPro: 2.711 ± 0.423
2.974LeuGln: 2.974 ± 0.612
2.886LeuArg: 2.886 ± 0.548
6.297LeuSer: 6.297 ± 0.822
5.51LeuThr: 5.51 ± 0.579
5.335LeuVal: 5.335 ± 0.595
0.875LeuTrp: 0.875 ± 0.293
3.061LeuTyr: 3.061 ± 0.467
0.0LeuXaa: 0.0 ± 0.0
Met
2.099MetAla: 2.099 ± 0.552
0.0MetCys: 0.0 ± 0.0
1.487MetAsp: 1.487 ± 0.313
1.399MetGlu: 1.399 ± 0.396
0.787MetPhe: 0.787 ± 0.235
0.612MetGly: 0.612 ± 0.195
0.175MetHis: 0.175 ± 0.107
1.487MetIle: 1.487 ± 0.399
2.799MetLys: 2.799 ± 0.581
1.574MetLeu: 1.574 ± 0.371
0.175MetMet: 0.175 ± 0.134
1.487MetAsn: 1.487 ± 0.333
0.525MetPro: 0.525 ± 0.178
1.224MetGln: 1.224 ± 0.39
0.7MetArg: 0.7 ± 0.311
1.312MetSer: 1.312 ± 0.308
2.186MetThr: 2.186 ± 0.378
1.137MetVal: 1.137 ± 0.331
0.35MetTrp: 0.35 ± 0.175
0.875MetTyr: 0.875 ± 0.271
0.0MetXaa: 0.0 ± 0.0
Asn
3.498AsnAla: 3.498 ± 0.632
0.262AsnCys: 0.262 ± 0.153
2.886AsnAsp: 2.886 ± 0.431
5.422AsnGlu: 5.422 ± 0.756
2.274AsnPhe: 2.274 ± 0.379
5.073AsnGly: 5.073 ± 0.613
1.224AsnHis: 1.224 ± 0.355
4.023AsnIle: 4.023 ± 0.797
5.51AsnLys: 5.51 ± 0.714
4.985AsnLeu: 4.985 ± 0.685
1.399AsnMet: 1.399 ± 0.432
3.936AsnAsn: 3.936 ± 0.553
2.186AsnPro: 2.186 ± 0.465
3.673AsnGln: 3.673 ± 0.554
2.099AsnArg: 2.099 ± 0.461
4.023AsnSer: 4.023 ± 0.579
3.498AsnThr: 3.498 ± 0.567
3.761AsnVal: 3.761 ± 0.576
1.137AsnTrp: 1.137 ± 0.3
2.974AsnTyr: 2.974 ± 0.473
0.0AsnXaa: 0.0 ± 0.0
Pro
1.924ProAla: 1.924 ± 0.436
0.087ProCys: 0.087 ± 0.078
1.574ProAsp: 1.574 ± 0.412
2.361ProGlu: 2.361 ± 0.368
1.137ProPhe: 1.137 ± 0.422
1.312ProGly: 1.312 ± 0.307
0.262ProHis: 0.262 ± 0.134
2.711ProIle: 2.711 ± 0.621
2.624ProLys: 2.624 ± 0.439
2.274ProLeu: 2.274 ± 0.479
0.35ProMet: 0.35 ± 0.145
1.924ProAsn: 1.924 ± 0.386
0.35ProPro: 0.35 ± 0.185
1.05ProGln: 1.05 ± 0.269
0.35ProArg: 0.35 ± 0.191
2.449ProSer: 2.449 ± 0.714
2.449ProThr: 2.449 ± 0.366
1.924ProVal: 1.924 ± 0.336
0.262ProTrp: 0.262 ± 0.142
0.787ProTyr: 0.787 ± 0.205
0.0ProXaa: 0.0 ± 0.0
Gln
2.711GlnAla: 2.711 ± 0.436
0.175GlnCys: 0.175 ± 0.1
1.224GlnAsp: 1.224 ± 0.285
2.012GlnGlu: 2.012 ± 0.412
2.012GlnPhe: 2.012 ± 0.383
2.711GlnGly: 2.711 ± 0.686
0.787GlnHis: 0.787 ± 0.227
2.624GlnIle: 2.624 ± 0.392
3.586GlnLys: 3.586 ± 0.637
3.673GlnLeu: 3.673 ± 0.647
1.312GlnMet: 1.312 ± 0.348
2.536GlnAsn: 2.536 ± 0.432
1.05GlnPro: 1.05 ± 0.488
1.837GlnGln: 1.837 ± 0.468
1.574GlnArg: 1.574 ± 0.512
2.536GlnSer: 2.536 ± 0.507
2.536GlnThr: 2.536 ± 0.487
1.574GlnVal: 1.574 ± 0.457
0.437GlnTrp: 0.437 ± 0.159
1.662GlnTyr: 1.662 ± 0.288
0.0GlnXaa: 0.0 ± 0.0
Arg
1.224ArgAla: 1.224 ± 0.34
0.087ArgCys: 0.087 ± 0.063
2.449ArgAsp: 2.449 ± 0.59
2.886ArgGlu: 2.886 ± 0.714
1.312ArgPhe: 1.312 ± 0.33
1.487ArgGly: 1.487 ± 0.367
0.35ArgHis: 0.35 ± 0.164
2.886ArgIle: 2.886 ± 0.568
3.586ArgLys: 3.586 ± 0.506
3.149ArgLeu: 3.149 ± 0.449
1.224ArgMet: 1.224 ± 0.455
2.974ArgAsn: 2.974 ± 0.424
1.05ArgPro: 1.05 ± 0.36
1.399ArgGln: 1.399 ± 0.32
1.487ArgArg: 1.487 ± 0.487
1.137ArgSer: 1.137 ± 0.308
2.799ArgThr: 2.799 ± 0.495
1.837ArgVal: 1.837 ± 0.286
0.262ArgTrp: 0.262 ± 0.133
1.574ArgTyr: 1.574 ± 0.35
0.0ArgXaa: 0.0 ± 0.0
Ser
5.335SerAla: 5.335 ± 0.819
0.262SerCys: 0.262 ± 0.138
5.248SerAsp: 5.248 ± 0.708
4.111SerGlu: 4.111 ± 0.707
2.099SerPhe: 2.099 ± 0.412
4.023SerGly: 4.023 ± 0.987
0.7SerHis: 0.7 ± 0.176
5.685SerIle: 5.685 ± 0.577
4.46SerLys: 4.46 ± 0.591
3.673SerLeu: 3.673 ± 0.587
1.487SerMet: 1.487 ± 0.454
4.285SerAsn: 4.285 ± 0.443
1.924SerPro: 1.924 ± 0.438
2.799SerGln: 2.799 ± 0.599
2.012SerArg: 2.012 ± 0.455
4.898SerSer: 4.898 ± 0.894
4.723SerThr: 4.723 ± 0.762
5.16SerVal: 5.16 ± 0.675
1.399SerTrp: 1.399 ± 0.402
2.799SerTyr: 2.799 ± 0.477
0.0SerXaa: 0.0 ± 0.0
Thr
5.51ThrAla: 5.51 ± 0.759
0.175ThrCys: 0.175 ± 0.151
4.023ThrAsp: 4.023 ± 0.845
3.586ThrGlu: 3.586 ± 0.557
2.799ThrPhe: 2.799 ± 0.46
4.985ThrGly: 4.985 ± 0.673
1.312ThrHis: 1.312 ± 0.292
6.384ThrIle: 6.384 ± 0.969
6.297ThrLys: 6.297 ± 0.769
6.21ThrLeu: 6.21 ± 0.891
1.224ThrMet: 1.224 ± 0.295
3.149ThrAsn: 3.149 ± 0.431
2.012ThrPro: 2.012 ± 0.428
2.624ThrGln: 2.624 ± 0.6
2.012ThrArg: 2.012 ± 0.37
4.723ThrSer: 4.723 ± 1.049
5.335ThrThr: 5.335 ± 1.111
5.248ThrVal: 5.248 ± 0.743
0.612ThrTrp: 0.612 ± 0.181
2.624ThrTyr: 2.624 ± 0.668
0.0ThrXaa: 0.0 ± 0.0
Val
4.373ValAla: 4.373 ± 0.612
0.262ValCys: 0.262 ± 0.123
4.548ValAsp: 4.548 ± 0.674
4.723ValGlu: 4.723 ± 0.629
1.837ValPhe: 1.837 ± 0.535
3.848ValGly: 3.848 ± 0.593
0.437ValHis: 0.437 ± 0.253
3.936ValIle: 3.936 ± 0.485
4.81ValLys: 4.81 ± 0.58
3.936ValLeu: 3.936 ± 0.605
1.837ValMet: 1.837 ± 0.296
3.323ValAsn: 3.323 ± 0.509
1.924ValPro: 1.924 ± 0.404
1.312ValGln: 1.312 ± 0.351
2.274ValArg: 2.274 ± 0.651
4.985ValSer: 4.985 ± 0.906
4.373ValThr: 4.373 ± 0.435
3.761ValVal: 3.761 ± 0.773
0.437ValTrp: 0.437 ± 0.222
2.099ValTyr: 2.099 ± 0.327
0.0ValXaa: 0.0 ± 0.0
Trp
0.7TrpAla: 0.7 ± 0.252
0.087TrpCys: 0.087 ± 0.086
0.525TrpAsp: 0.525 ± 0.188
1.137TrpGlu: 1.137 ± 0.289
0.875TrpPhe: 0.875 ± 0.24
1.05TrpGly: 1.05 ± 0.255
0.175TrpHis: 0.175 ± 0.1
0.875TrpIle: 0.875 ± 0.225
1.137TrpLys: 1.137 ± 0.319
0.962TrpLeu: 0.962 ± 0.35
0.0TrpMet: 0.0 ± 0.0
0.437TrpAsn: 0.437 ± 0.26
0.087TrpPro: 0.087 ± 0.092
0.262TrpGln: 0.262 ± 0.153
0.35TrpArg: 0.35 ± 0.168
0.7TrpSer: 0.7 ± 0.243
1.399TrpThr: 1.399 ± 0.48
0.612TrpVal: 0.612 ± 0.244
0.175TrpTrp: 0.175 ± 0.103
0.525TrpTyr: 0.525 ± 0.213
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.799TyrAla: 2.799 ± 0.697
0.35TyrCys: 0.35 ± 0.153
2.624TyrAsp: 2.624 ± 0.504
2.711TyrGlu: 2.711 ± 0.453
1.749TyrPhe: 1.749 ± 0.402
2.361TyrGly: 2.361 ± 0.414
0.612TyrHis: 0.612 ± 0.244
2.361TyrIle: 2.361 ± 0.523
3.498TyrLys: 3.498 ± 0.4
3.323TyrLeu: 3.323 ± 0.593
0.7TyrMet: 0.7 ± 0.244
2.449TyrAsn: 2.449 ± 0.511
1.224TyrPro: 1.224 ± 0.39
1.837TyrGln: 1.837 ± 0.322
1.749TyrArg: 1.749 ± 0.425
2.012TyrSer: 2.012 ± 0.446
3.149TyrThr: 3.149 ± 0.604
2.274TyrVal: 2.274 ± 0.443
0.175TyrTrp: 0.175 ± 0.126
1.837TyrTyr: 1.837 ± 0.424
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (11435 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski