Psychromonas sp. RZ5

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Psychromonadaceae; Psychromonas; unclassified Psychromonas

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3176 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y7ZIL1|A0A4Y7ZIL1_9GAMM 1 4-dihydroxy-2-naphthoate octaprenyltransferase OS=Psychromonas sp. RZ5 OX=2555642 GN=menA PE=3 SV=1
MM1 pKa = 7.52KK2 pKa = 10.3KK3 pKa = 10.46ALLAVLIPSLLGATSAFAGGVDD25 pKa = 5.4LIKK28 pKa = 10.95NDD30 pKa = 4.68DD31 pKa = 3.42MTLNFNGDD39 pKa = 3.49IDD41 pKa = 4.43LKK43 pKa = 11.25SYY45 pKa = 8.97ITFTDD50 pKa = 2.87TDD52 pKa = 3.54GDD54 pKa = 4.04YY55 pKa = 11.04KK56 pKa = 11.35SNSGEE61 pKa = 4.13TEE63 pKa = 4.15VIFDD67 pKa = 5.38DD68 pKa = 5.01IDD70 pKa = 4.85FKK72 pKa = 10.45FTWHH76 pKa = 7.05ASDD79 pKa = 3.67NLDD82 pKa = 4.18YY83 pKa = 10.92IAGMDD88 pKa = 4.12LTADD92 pKa = 3.62PDD94 pKa = 4.21GYY96 pKa = 9.43TEE98 pKa = 4.02IGAAVGVFYY107 pKa = 10.77AWAGFNHH114 pKa = 5.84SQYY117 pKa = 11.55GQLTWGNQITSFDD130 pKa = 3.54NFGIDD135 pKa = 3.33TAEE138 pKa = 3.68IWGGAASGKK147 pKa = 10.17LDD149 pKa = 3.47GRR151 pKa = 11.84STEE154 pKa = 3.82HH155 pKa = 6.91SNALVYY161 pKa = 10.42EE162 pKa = 4.05IEE164 pKa = 4.57IDD166 pKa = 3.91DD167 pKa = 3.99LTISATYY174 pKa = 10.01GINNEE179 pKa = 4.61DD180 pKa = 4.91DD181 pKa = 4.35SDD183 pKa = 3.95DD184 pKa = 3.88FKK186 pKa = 11.27NAKK189 pKa = 8.96VMQVTAFYY197 pKa = 10.81KK198 pKa = 10.76PGDD201 pKa = 3.69LQINAGVGHH210 pKa = 4.91TTGYY214 pKa = 9.83NQDD217 pKa = 3.56SADD220 pKa = 3.61FAQVSTAVYY229 pKa = 8.68GQAEE233 pKa = 4.49VEE235 pKa = 4.24YY236 pKa = 10.52QMGDD240 pKa = 2.72ISVAGLVAYY249 pKa = 10.29QDD251 pKa = 3.32AAYY254 pKa = 9.68EE255 pKa = 4.26DD256 pKa = 4.3NSANTGSDD264 pKa = 3.45FTSTGVEE271 pKa = 3.63FDD273 pKa = 3.37VTYY276 pKa = 10.94KK277 pKa = 10.85LSSTVKK283 pKa = 10.02LSVGGDD289 pKa = 3.98YY290 pKa = 9.6ITQDD294 pKa = 2.86IDD296 pKa = 3.61NVSDD300 pKa = 3.21NDD302 pKa = 3.65AYY304 pKa = 10.88MDD306 pKa = 4.14LFVASKK312 pKa = 8.72HH313 pKa = 4.89TLTKK317 pKa = 10.45NVYY320 pKa = 10.19LLTEE324 pKa = 4.41LAMQQGDD331 pKa = 4.37LIEE334 pKa = 4.1YY335 pKa = 10.05RR336 pKa = 11.84ADD338 pKa = 3.45KK339 pKa = 10.97DD340 pKa = 4.22SVSTTSLDD348 pKa = 3.57DD349 pKa = 3.99AQVKK353 pKa = 10.48VGFLLNMQFF362 pKa = 4.04

Molecular weight: 39.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y7ZMG5|A0A4Y7ZMG5_9GAMM Single-stranded-DNA-specific exonuclease RecJ OS=Psychromonas sp. RZ5 OX=2555642 GN=recJ PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNTKK11 pKa = 10.0RR12 pKa = 11.84KK13 pKa = 8.73RR14 pKa = 11.84SHH16 pKa = 6.16GFRR19 pKa = 11.84TRR21 pKa = 11.84MATKK25 pKa = 10.18NGRR28 pKa = 11.84AVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.5GRR39 pKa = 11.84ASLSAA44 pKa = 3.83

Molecular weight: 5.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3176

0

3176

992658

32

2661

312.5

34.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.078 ± 0.047

1.033 ± 0.015

5.45 ± 0.037

6.417 ± 0.049

4.422 ± 0.033

6.201 ± 0.046

2.098 ± 0.022

7.286 ± 0.038

6.218 ± 0.042

10.704 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.42 ± 0.02

4.845 ± 0.036

3.497 ± 0.026

4.508 ± 0.041

3.778 ± 0.034

6.818 ± 0.044

5.436 ± 0.045

6.591 ± 0.036

1.087 ± 0.015

3.113 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski