Amino acid dipepetide frequency for Psychromonas sp. RZ5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.636AlaAla: 6.636 ± 0.112
0.923AlaCys: 0.923 ± 0.034
4.222AlaAsp: 4.222 ± 0.081
5.33AlaGlu: 5.33 ± 0.09
3.494AlaPhe: 3.494 ± 0.068
5.137AlaGly: 5.137 ± 0.081
1.455AlaHis: 1.455 ± 0.038
6.289AlaIle: 6.289 ± 0.089
5.171AlaLys: 5.171 ± 0.077
9.298AlaLeu: 9.298 ± 0.115
2.28AlaMet: 2.28 ± 0.053
3.689AlaAsn: 3.689 ± 0.064
2.45AlaPro: 2.45 ± 0.055
3.404AlaGln: 3.404 ± 0.064
2.868AlaArg: 2.868 ± 0.062
5.228AlaSer: 5.228 ± 0.088
4.312AlaThr: 4.312 ± 0.071
5.344AlaVal: 5.344 ± 0.077
0.809AlaTrp: 0.809 ± 0.031
2.446AlaTyr: 2.446 ± 0.06
0.0AlaXaa: 0.0 ± 0.0
Cys
0.759CysAla: 0.759 ± 0.028
0.171CysCys: 0.171 ± 0.015
0.597CysAsp: 0.597 ± 0.028
0.652CysGlu: 0.652 ± 0.027
0.532CysPhe: 0.532 ± 0.025
0.807CysGly: 0.807 ± 0.03
0.313CysHis: 0.313 ± 0.019
0.709CysIle: 0.709 ± 0.022
0.554CysLys: 0.554 ± 0.026
1.075CysLeu: 1.075 ± 0.037
0.202CysMet: 0.202 ± 0.017
0.413CysAsn: 0.413 ± 0.022
0.419CysPro: 0.419 ± 0.023
0.398CysGln: 0.398 ± 0.024
0.393CysArg: 0.393 ± 0.025
0.734CysSer: 0.734 ± 0.029
0.471CysThr: 0.471 ± 0.024
0.625CysVal: 0.625 ± 0.027
0.12CysTrp: 0.12 ± 0.011
0.39CysTyr: 0.39 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
4.213AspAla: 4.213 ± 0.074
0.571AspCys: 0.571 ± 0.028
3.01AspAsp: 3.01 ± 0.083
3.729AspGlu: 3.729 ± 0.069
2.729AspPhe: 2.729 ± 0.055
3.276AspGly: 3.276 ± 0.068
1.065AspHis: 1.065 ± 0.03
4.312AspIle: 4.312 ± 0.077
3.714AspLys: 3.714 ± 0.058
5.641AspLeu: 5.641 ± 0.079
1.254AspMet: 1.254 ± 0.033
2.719AspAsn: 2.719 ± 0.054
1.925AspPro: 1.925 ± 0.045
2.168AspGln: 2.168 ± 0.048
1.888AspArg: 1.888 ± 0.051
3.34AspSer: 3.34 ± 0.074
2.561AspThr: 2.561 ± 0.055
3.576AspVal: 3.576 ± 0.072
0.746AspTrp: 0.746 ± 0.027
2.062AspTyr: 2.062 ± 0.05
0.0AspXaa: 0.0 ± 0.0
Glu
5.134GluAla: 5.134 ± 0.091
0.587GluCys: 0.587 ± 0.028
3.007GluAsp: 3.007 ± 0.068
3.993GluGlu: 3.993 ± 0.1
2.645GluPhe: 2.645 ± 0.052
3.496GluGly: 3.496 ± 0.073
1.531GluHis: 1.531 ± 0.039
4.465GluIle: 4.465 ± 0.081
4.481GluLys: 4.481 ± 0.067
7.155GluLeu: 7.155 ± 0.104
1.742GluMet: 1.742 ± 0.042
3.346GluAsn: 3.346 ± 0.063
1.669GluPro: 1.669 ± 0.05
4.022GluGln: 4.022 ± 0.074
2.643GluArg: 2.643 ± 0.06
4.02GluSer: 4.02 ± 0.078
3.405GluThr: 3.405 ± 0.072
4.339GluVal: 4.339 ± 0.077
0.666GluTrp: 0.666 ± 0.027
1.827GluTyr: 1.827 ± 0.045
0.0GluXaa: 0.0 ± 0.0
Phe
3.576PheAla: 3.576 ± 0.07
0.564PheCys: 0.564 ± 0.024
2.814PheAsp: 2.814 ± 0.049
2.691PheGlu: 2.691 ± 0.046
1.965PhePhe: 1.965 ± 0.055
2.757PheGly: 2.757 ± 0.062
0.845PheHis: 0.845 ± 0.029
3.598PheIle: 3.598 ± 0.068
2.649PheLys: 2.649 ± 0.054
3.96PheLeu: 3.96 ± 0.077
1.014PheMet: 1.014 ± 0.034
2.443PheAsn: 2.443 ± 0.052
1.467PhePro: 1.467 ± 0.036
1.277PheGln: 1.277 ± 0.037
1.316PheArg: 1.316 ± 0.039
3.766PheSer: 3.766 ± 0.066
2.569PheThr: 2.569 ± 0.056
2.923PheVal: 2.923 ± 0.053
0.487PheTrp: 0.487 ± 0.024
1.54PheTyr: 1.54 ± 0.049
0.0PheXaa: 0.0 ± 0.0
Gly
4.585GlyAla: 4.585 ± 0.082
0.826GlyCys: 0.826 ± 0.031
3.408GlyAsp: 3.408 ± 0.071
4.21GlyGlu: 4.21 ± 0.07
3.245GlyPhe: 3.245 ± 0.059
4.0GlyGly: 4.0 ± 0.079
1.352GlyHis: 1.352 ± 0.039
4.741GlyIle: 4.741 ± 0.086
4.057GlyLys: 4.057 ± 0.072
6.612GlyLeu: 6.612 ± 0.094
1.727GlyMet: 1.727 ± 0.049
2.49GlyAsn: 2.49 ± 0.06
1.391GlyPro: 1.391 ± 0.035
2.409GlyGln: 2.409 ± 0.05
2.442GlyArg: 2.442 ± 0.052
3.761GlySer: 3.761 ± 0.071
3.069GlyThr: 3.069 ± 0.069
4.53GlyVal: 4.53 ± 0.074
0.768GlyTrp: 0.768 ± 0.028
2.392GlyTyr: 2.392 ± 0.052
0.0GlyXaa: 0.0 ± 0.0
His
1.424HisAla: 1.424 ± 0.039
0.315HisCys: 0.315 ± 0.017
1.026HisAsp: 1.026 ± 0.036
1.13HisGlu: 1.13 ± 0.039
1.204HisPhe: 1.204 ± 0.036
1.321HisGly: 1.321 ± 0.04
0.623HisHis: 0.623 ± 0.029
1.477HisIle: 1.477 ± 0.035
1.129HisLys: 1.129 ± 0.03
2.319HisLeu: 2.319 ± 0.056
0.409HisMet: 0.409 ± 0.021
0.925HisAsn: 0.925 ± 0.034
1.009HisPro: 1.009 ± 0.039
1.163HisGln: 1.163 ± 0.037
0.893HisArg: 0.893 ± 0.031
1.426HisSer: 1.426 ± 0.034
0.923HisThr: 0.923 ± 0.032
1.076HisVal: 1.076 ± 0.036
0.304HisTrp: 0.304 ± 0.014
0.881HisTyr: 0.881 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
6.714IleAla: 6.714 ± 0.094
0.751IleCys: 0.751 ± 0.028
4.728IleAsp: 4.728 ± 0.075
5.484IleGlu: 5.484 ± 0.087
2.775IlePhe: 2.775 ± 0.062
4.771IleGly: 4.771 ± 0.077
1.316IleHis: 1.316 ± 0.036
4.909IleIle: 4.909 ± 0.079
4.834IleLys: 4.834 ± 0.078
6.166IleLeu: 6.166 ± 0.099
1.443IleMet: 1.443 ± 0.042
4.137IleAsn: 4.137 ± 0.065
2.736IlePro: 2.736 ± 0.056
2.642IleGln: 2.642 ± 0.058
2.607IleArg: 2.607 ± 0.052
5.322IleSer: 5.322 ± 0.084
4.356IleThr: 4.356 ± 0.063
4.448IleVal: 4.448 ± 0.07
0.614IleTrp: 0.614 ± 0.025
2.103IleTyr: 2.103 ± 0.048
0.0IleXaa: 0.0 ± 0.0
Lys
5.223LysAla: 5.223 ± 0.092
0.367LysCys: 0.367 ± 0.017
3.081LysAsp: 3.081 ± 0.057
4.244LysGlu: 4.244 ± 0.077
1.926LysPhe: 1.926 ± 0.036
3.538LysGly: 3.538 ± 0.061
1.433LysHis: 1.433 ± 0.04
4.141LysIle: 4.141 ± 0.062
4.366LysLys: 4.366 ± 0.087
6.236LysLeu: 6.236 ± 0.085
1.737LysMet: 1.737 ± 0.043
3.228LysAsn: 3.228 ± 0.059
2.232LysPro: 2.232 ± 0.049
3.713LysGln: 3.713 ± 0.074
2.793LysArg: 2.793 ± 0.056
3.908LysSer: 3.908 ± 0.075
3.463LysThr: 3.463 ± 0.066
4.268LysVal: 4.268 ± 0.074
0.618LysTrp: 0.618 ± 0.024
1.668LysTyr: 1.668 ± 0.044
0.0LysXaa: 0.0 ± 0.0
Leu
9.193LeuAla: 9.193 ± 0.107
1.048LeuCys: 1.048 ± 0.032
5.628LeuAsp: 5.628 ± 0.094
5.856LeuGlu: 5.856 ± 0.09
4.926LeuPhe: 4.926 ± 0.094
6.496LeuGly: 6.496 ± 0.089
2.044LeuHis: 2.044 ± 0.05
7.684LeuIle: 7.684 ± 0.096
6.383LeuLys: 6.383 ± 0.097
11.833LeuLeu: 11.833 ± 0.162
2.599LeuMet: 2.599 ± 0.054
5.36LeuAsn: 5.36 ± 0.09
4.495LeuPro: 4.495 ± 0.066
4.366LeuGln: 4.366 ± 0.078
3.807LeuArg: 3.807 ± 0.06
8.385LeuSer: 8.385 ± 0.106
6.611LeuThr: 6.611 ± 0.085
6.565LeuVal: 6.565 ± 0.093
0.975LeuTrp: 0.975 ± 0.035
2.789LeuTyr: 2.789 ± 0.056
0.0LeuXaa: 0.0 ± 0.0
Met
2.063MetAla: 2.063 ± 0.042
0.194MetCys: 0.194 ± 0.014
1.17MetAsp: 1.17 ± 0.033
1.202MetGlu: 1.202 ± 0.034
0.955MetPhe: 0.955 ± 0.035
1.62MetGly: 1.62 ± 0.041
0.47MetHis: 0.47 ± 0.019
1.642MetIle: 1.642 ± 0.045
1.574MetLys: 1.574 ± 0.041
2.682MetLeu: 2.682 ± 0.06
0.666MetMet: 0.666 ± 0.027
1.212MetAsn: 1.212 ± 0.036
1.077MetPro: 1.077 ± 0.034
1.221MetGln: 1.221 ± 0.036
0.993MetArg: 0.993 ± 0.031
1.895MetSer: 1.895 ± 0.044
1.366MetThr: 1.366 ± 0.039
1.545MetVal: 1.545 ± 0.038
0.19MetTrp: 0.19 ± 0.016
0.458MetTyr: 0.458 ± 0.023
0.0MetXaa: 0.0 ± 0.0
Asn
3.683AsnAla: 3.683 ± 0.062
0.456AsnCys: 0.456 ± 0.026
2.677AsnAsp: 2.677 ± 0.059
3.119AsnGlu: 3.119 ± 0.061
1.811AsnPhe: 1.811 ± 0.042
3.001AsnGly: 3.001 ± 0.064
0.996AsnHis: 0.996 ± 0.031
3.867AsnIle: 3.867 ± 0.053
3.58AsnLys: 3.58 ± 0.062
4.542AsnLeu: 4.542 ± 0.077
1.077AsnMet: 1.077 ± 0.037
2.863AsnAsn: 2.863 ± 0.067
1.961AsnPro: 1.961 ± 0.047
2.31AsnGln: 2.31 ± 0.053
1.731AsnArg: 1.731 ± 0.041
3.069AsnSer: 3.069 ± 0.071
2.67AsnThr: 2.67 ± 0.063
2.907AsnVal: 2.907 ± 0.056
0.585AsnTrp: 0.585 ± 0.028
1.54AsnTyr: 1.54 ± 0.042
0.0AsnXaa: 0.0 ± 0.0
Pro
2.689ProAla: 2.689 ± 0.061
0.323ProCys: 0.323 ± 0.02
1.999ProAsp: 1.999 ± 0.049
3.04ProGlu: 3.04 ± 0.056
1.703ProPhe: 1.703 ± 0.048
1.783ProGly: 1.783 ± 0.043
0.726ProHis: 0.726 ± 0.028
2.645ProIle: 2.645 ± 0.051
2.002ProLys: 2.002 ± 0.05
3.9ProLeu: 3.9 ± 0.057
0.81ProMet: 0.81 ± 0.031
1.628ProAsn: 1.628 ± 0.04
0.906ProPro: 0.906 ± 0.036
1.301ProGln: 1.301 ± 0.033
1.118ProArg: 1.118 ± 0.032
2.28ProSer: 2.28 ± 0.051
1.957ProThr: 1.957 ± 0.045
2.621ProVal: 2.621 ± 0.061
0.404ProTrp: 0.404 ± 0.022
1.135ProTyr: 1.135 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
4.071GlnAla: 4.071 ± 0.078
0.4GlnCys: 0.4 ± 0.019
2.051GlnAsp: 2.051 ± 0.042
2.481GlnGlu: 2.481 ± 0.054
1.753GlnPhe: 1.753 ± 0.042
2.802GlnGly: 2.802 ± 0.054
1.129GlnHis: 1.129 ± 0.036
2.8GlnIle: 2.8 ± 0.06
2.661GlnLys: 2.661 ± 0.055
5.368GlnLeu: 5.368 ± 0.098
0.948GlnMet: 0.948 ± 0.033
1.691GlnAsn: 1.691 ± 0.039
1.358GlnPro: 1.358 ± 0.039
3.278GlnGln: 3.278 ± 0.102
2.003GlnArg: 2.003 ± 0.046
2.875GlnSer: 2.875 ± 0.067
2.359GlnThr: 2.359 ± 0.058
3.062GlnVal: 3.062 ± 0.065
0.606GlnTrp: 0.606 ± 0.025
1.386GlnTyr: 1.386 ± 0.044
0.0GlnXaa: 0.0 ± 0.0
Arg
2.627ArgAla: 2.627 ± 0.055
0.406ArgCys: 0.406 ± 0.02
2.058ArgAsp: 2.058 ± 0.042
2.392ArgGlu: 2.392 ± 0.052
2.001ArgPhe: 2.001 ± 0.049
2.16ArgGly: 2.16 ± 0.043
0.89ArgHis: 0.89 ± 0.03
2.793ArgIle: 2.793 ± 0.045
2.137ArgLys: 2.137 ± 0.054
4.412ArgLeu: 4.412 ± 0.071
0.883ArgMet: 0.883 ± 0.031
1.722ArgAsn: 1.722 ± 0.043
1.339ArgPro: 1.339 ± 0.039
1.561ArgGln: 1.561 ± 0.041
1.713ArgArg: 1.713 ± 0.05
2.426ArgSer: 2.426 ± 0.052
1.781ArgThr: 1.781 ± 0.044
2.516ArgVal: 2.516 ± 0.057
0.44ArgTrp: 0.44 ± 0.023
1.52ArgTyr: 1.52 ± 0.039
0.0ArgXaa: 0.0 ± 0.0
Ser
5.166SerAla: 5.166 ± 0.077
0.632SerCys: 0.632 ± 0.025
3.816SerAsp: 3.816 ± 0.07
4.487SerGlu: 4.487 ± 0.079
3.201SerPhe: 3.201 ± 0.064
4.573SerGly: 4.573 ± 0.083
1.448SerHis: 1.448 ± 0.044
5.087SerIle: 5.087 ± 0.075
3.834SerLys: 3.834 ± 0.063
7.731SerLeu: 7.731 ± 0.107
1.661SerMet: 1.661 ± 0.04
3.159SerAsn: 3.159 ± 0.074
2.244SerPro: 2.244 ± 0.049
2.741SerGln: 2.741 ± 0.061
2.477SerArg: 2.477 ± 0.057
4.742SerSer: 4.742 ± 0.099
3.619SerThr: 3.619 ± 0.072
4.644SerVal: 4.644 ± 0.062
0.752SerTrp: 0.752 ± 0.033
2.164SerTyr: 2.164 ± 0.052
0.0SerXaa: 0.0 ± 0.0
Thr
4.364ThrAla: 4.364 ± 0.074
0.487ThrCys: 0.487 ± 0.023
2.975ThrAsp: 2.975 ± 0.066
3.568ThrGlu: 3.568 ± 0.076
2.305ThrPhe: 2.305 ± 0.051
3.753ThrGly: 3.753 ± 0.071
1.179ThrHis: 1.179 ± 0.037
3.882ThrIle: 3.882 ± 0.073
2.762ThrLys: 2.762 ± 0.06
6.486ThrLeu: 6.486 ± 0.085
1.12ThrMet: 1.12 ± 0.035
2.405ThrAsn: 2.405 ± 0.063
2.425ThrPro: 2.425 ± 0.047
2.405ThrGln: 2.405 ± 0.06
2.014ThrArg: 2.014 ± 0.047
3.476ThrSer: 3.476 ± 0.06
3.065ThrThr: 3.065 ± 0.069
3.588ThrVal: 3.588 ± 0.071
0.593ThrTrp: 0.593 ± 0.024
1.508ThrTyr: 1.508 ± 0.039
0.0ThrXaa: 0.0 ± 0.0
Val
5.683ValAla: 5.683 ± 0.086
0.72ValCys: 0.72 ± 0.028
4.033ValAsp: 4.033 ± 0.065
4.416ValGlu: 4.416 ± 0.083
2.854ValPhe: 2.854 ± 0.053
4.105ValGly: 4.105 ± 0.076
1.17ValHis: 1.17 ± 0.034
5.039ValIle: 5.039 ± 0.079
3.954ValLys: 3.954 ± 0.073
6.571ValLeu: 6.571 ± 0.09
1.687ValMet: 1.687 ± 0.043
3.3ValAsn: 3.3 ± 0.06
2.179ValPro: 2.179 ± 0.052
2.025ValGln: 2.025 ± 0.051
2.204ValArg: 2.204 ± 0.049
4.727ValSer: 4.727 ± 0.083
3.864ValThr: 3.864 ± 0.071
4.878ValVal: 4.878 ± 0.083
0.627ValTrp: 0.627 ± 0.025
1.876ValTyr: 1.876 ± 0.04
0.0ValXaa: 0.0 ± 0.0
Trp
0.718TrpAla: 0.718 ± 0.029
0.147TrpCys: 0.147 ± 0.011
0.553TrpAsp: 0.553 ± 0.029
0.499TrpGlu: 0.499 ± 0.022
0.572TrpPhe: 0.572 ± 0.03
0.681TrpGly: 0.681 ± 0.026
0.261TrpHis: 0.261 ± 0.016
0.721TrpIle: 0.721 ± 0.029
0.566TrpLys: 0.566 ± 0.021
1.478TrpLeu: 1.478 ± 0.043
0.303TrpMet: 0.303 ± 0.016
0.427TrpAsn: 0.427 ± 0.022
0.388TrpPro: 0.388 ± 0.02
0.7TrpGln: 0.7 ± 0.029
0.496TrpArg: 0.496 ± 0.021
0.688TrpSer: 0.688 ± 0.03
0.431TrpThr: 0.431 ± 0.018
0.765TrpVal: 0.765 ± 0.031
0.142TrpTrp: 0.142 ± 0.014
0.331TrpTyr: 0.331 ± 0.024
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.264TyrAla: 2.264 ± 0.055
0.446TyrCys: 0.446 ± 0.024
1.649TyrAsp: 1.649 ± 0.047
1.648TyrGlu: 1.648 ± 0.043
1.627TyrPhe: 1.627 ± 0.038
1.937TyrGly: 1.937 ± 0.045
0.732TyrHis: 0.732 ± 0.025
2.055TyrIle: 2.055 ± 0.044
1.774TyrLys: 1.774 ± 0.045
3.578TyrLeu: 3.578 ± 0.063
0.633TyrMet: 0.633 ± 0.026
1.289TyrAsn: 1.289 ± 0.034
1.298TyrPro: 1.298 ± 0.038
1.98TyrGln: 1.98 ± 0.051
1.379TyrArg: 1.379 ± 0.036
2.113TyrSer: 2.113 ± 0.049
1.506TyrThr: 1.506 ± 0.05
1.689TyrVal: 1.689 ± 0.042
0.422TyrTrp: 0.422 ± 0.022
1.109TyrTyr: 1.109 ± 0.035
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3176 proteins (992658 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski