Tortoise microvirus 23

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4P8W5R3|A0A4P8W5R3_9VIRU DNA pilot protein OS=Tortoise microvirus 23 OX=2583125 PE=4 SV=1
MM1 pKa = 7.65ANHH4 pKa = 6.28EE5 pKa = 4.49PDD7 pKa = 4.17YY8 pKa = 10.19EE9 pKa = 4.37TLDD12 pKa = 3.85SVSVTVPDD20 pKa = 3.55MALSVSEE27 pKa = 3.72IVRR30 pKa = 11.84RR31 pKa = 11.84SRR33 pKa = 11.84AGIPVSVSQRR43 pKa = 11.84NPTNIPEE50 pKa = 4.11NRR52 pKa = 11.84DD53 pKa = 3.39FFDD56 pKa = 4.32LCDD59 pKa = 3.59SLPYY63 pKa = 9.76YY64 pKa = 10.75DD65 pKa = 4.84KK66 pKa = 11.06TIADD70 pKa = 4.35FKK72 pKa = 11.01KK73 pKa = 10.0SQQKK77 pKa = 10.18PSEE80 pKa = 4.4TPQTPNPAPTSMVEE94 pKa = 3.98RR95 pKa = 11.84SEE97 pKa = 4.22EE98 pKa = 3.85

Molecular weight:
11.0 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4V1FVX3|A0A4V1FVX3_9VIRU Replication initiation protein OS=Tortoise microvirus 23 OX=2583125 PE=4 SV=1
MM1 pKa = 7.47ACISPILIKK10 pKa = 10.91SPLTPDD16 pKa = 2.76SHH18 pKa = 8.88IYY20 pKa = 9.22VPCGRR25 pKa = 11.84CAWCRR30 pKa = 11.84RR31 pKa = 11.84LMQTDD36 pKa = 4.14WYY38 pKa = 10.99CRR40 pKa = 11.84FLEE43 pKa = 4.1EE44 pKa = 4.43RR45 pKa = 11.84KK46 pKa = 9.8EE47 pKa = 4.07NPVVSFLTLTYY58 pKa = 10.59SPEE61 pKa = 4.1NLPLMCDD68 pKa = 3.73DD69 pKa = 3.94LTGEE73 pKa = 4.45IKK75 pKa = 10.59SVAQKK80 pKa = 9.94TDD82 pKa = 2.54IQKK85 pKa = 10.09YY86 pKa = 8.24IKK88 pKa = 10.22RR89 pKa = 11.84LRR91 pKa = 11.84KK92 pKa = 8.82EE93 pKa = 3.85NKK95 pKa = 6.46TFKK98 pKa = 10.22YY99 pKa = 10.12FVVSEE104 pKa = 4.11HH105 pKa = 6.14GKK107 pKa = 10.25KK108 pKa = 7.94ITKK111 pKa = 9.44RR112 pKa = 11.84VHH114 pKa = 4.59YY115 pKa = 9.09HH116 pKa = 6.38ALMFSKK122 pKa = 8.55THH124 pKa = 6.58YY125 pKa = 10.14DD126 pKa = 3.72YY127 pKa = 11.35QSQWTKK133 pKa = 10.38GFSMQLPATEE143 pKa = 3.96GAMRR147 pKa = 11.84YY148 pKa = 4.75VTKK151 pKa = 10.87YY152 pKa = 9.31ILKK155 pKa = 9.8GSEE158 pKa = 3.78RR159 pKa = 11.84DD160 pKa = 3.7TEE162 pKa = 4.65IMLCSKK168 pKa = 10.35KK169 pKa = 10.02PAIGSSYY176 pKa = 9.66LTSQMIDD183 pKa = 3.28YY184 pKa = 9.98QLQKK188 pKa = 11.07DD189 pKa = 4.64EE190 pKa = 4.6PCYY193 pKa = 8.63THH195 pKa = 7.38PGGAVSPLPRR205 pKa = 11.84YY206 pKa = 8.79YY207 pKa = 10.34RR208 pKa = 11.84KK209 pKa = 10.45KK210 pKa = 9.33IWNEE214 pKa = 3.56STLPHH219 pKa = 6.27YY220 pKa = 11.11LEE222 pKa = 4.19THH224 pKa = 6.36KK225 pKa = 10.52NTILEE230 pKa = 4.32TMEE233 pKa = 4.91SSIKK237 pKa = 10.66HH238 pKa = 4.19NTLIRR243 pKa = 11.84RR244 pKa = 11.84YY245 pKa = 8.65KK246 pKa = 9.7EE247 pKa = 3.92KK248 pKa = 10.94YY249 pKa = 9.55GSDD252 pKa = 2.86EE253 pKa = 5.28GFYY256 pKa = 11.12DD257 pKa = 3.59WLNAHH262 pKa = 6.38YY263 pKa = 10.41EE264 pKa = 4.02RR265 pKa = 11.84DD266 pKa = 3.38LKK268 pKa = 10.69IQHH271 pKa = 6.95KK272 pKa = 10.15INNSS276 pKa = 3.16

Molecular weight:
32.58 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1250

98

524

250.0

28.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.36 ± 0.98

1.12 ± 0.431

5.28 ± 0.429

6.72 ± 0.516

5.28 ± 1.614

5.04 ± 0.857

2.08 ± 0.647

4.96 ± 0.426

6.4 ± 1.123

8.64 ± 0.606

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.4 ± 0.347

6.32 ± 1.129

5.36 ± 0.743

4.96 ± 1.014

5.28 ± 0.384

7.6 ± 1.041

5.12 ± 1.043

5.2 ± 0.818

1.44 ± 0.173

5.44 ± 0.628

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski