Tortoise microvirus 23
Average proteome isoelectric point is 6.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W5R3|A0A4P8W5R3_9VIRU DNA pilot protein OS=Tortoise microvirus 23 OX=2583125 PE=4 SV=1
MM1 pKa = 7.65 ANHH4 pKa = 6.28 EE5 pKa = 4.49 PDD7 pKa = 4.17 YY8 pKa = 10.19 EE9 pKa = 4.37 TLDD12 pKa = 3.85 SVSVTVPDD20 pKa = 3.55 MALSVSEE27 pKa = 3.72 IVRR30 pKa = 11.84 RR31 pKa = 11.84 SRR33 pKa = 11.84 AGIPVSVSQRR43 pKa = 11.84 NPTNIPEE50 pKa = 4.11 NRR52 pKa = 11.84 DD53 pKa = 3.39 FFDD56 pKa = 4.32 LCDD59 pKa = 3.59 SLPYY63 pKa = 9.76 YY64 pKa = 10.75 DD65 pKa = 4.84 KK66 pKa = 11.06 TIADD70 pKa = 4.35 FKK72 pKa = 11.01 KK73 pKa = 10.0 SQQKK77 pKa = 10.18 PSEE80 pKa = 4.4 TPQTPNPAPTSMVEE94 pKa = 3.98 RR95 pKa = 11.84 SEE97 pKa = 4.22 EE98 pKa = 3.85
Molecular weight: 11.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.671
IPC2_protein 4.647
IPC_protein 4.507
Toseland 4.342
ProMoST 4.546
Dawson 4.469
Bjellqvist 4.673
Wikipedia 4.355
Rodwell 4.342
Grimsley 4.266
Solomon 4.457
Lehninger 4.406
Nozaki 4.571
DTASelect 4.749
Thurlkill 4.368
EMBOSS 4.368
Sillero 4.622
Patrickios 4.05
IPC_peptide 4.457
IPC2_peptide 4.609
IPC2.peptide.svr19 4.561
Protein with the highest isoelectric point:
>tr|A0A4V1FVX3|A0A4V1FVX3_9VIRU Replication initiation protein OS=Tortoise microvirus 23 OX=2583125 PE=4 SV=1
MM1 pKa = 7.47 ACISPILIKK10 pKa = 10.91 SPLTPDD16 pKa = 2.76 SHH18 pKa = 8.88 IYY20 pKa = 9.22 VPCGRR25 pKa = 11.84 CAWCRR30 pKa = 11.84 RR31 pKa = 11.84 LMQTDD36 pKa = 4.14 WYY38 pKa = 10.99 CRR40 pKa = 11.84 FLEE43 pKa = 4.1 EE44 pKa = 4.43 RR45 pKa = 11.84 KK46 pKa = 9.8 EE47 pKa = 4.07 NPVVSFLTLTYY58 pKa = 10.59 SPEE61 pKa = 4.1 NLPLMCDD68 pKa = 3.73 DD69 pKa = 3.94 LTGEE73 pKa = 4.45 IKK75 pKa = 10.59 SVAQKK80 pKa = 9.94 TDD82 pKa = 2.54 IQKK85 pKa = 10.09 YY86 pKa = 8.24 IKK88 pKa = 10.22 RR89 pKa = 11.84 LRR91 pKa = 11.84 KK92 pKa = 8.82 EE93 pKa = 3.85 NKK95 pKa = 6.46 TFKK98 pKa = 10.22 YY99 pKa = 10.12 FVVSEE104 pKa = 4.11 HH105 pKa = 6.14 GKK107 pKa = 10.25 KK108 pKa = 7.94 ITKK111 pKa = 9.44 RR112 pKa = 11.84 VHH114 pKa = 4.59 YY115 pKa = 9.09 HH116 pKa = 6.38 ALMFSKK122 pKa = 8.55 THH124 pKa = 6.58 YY125 pKa = 10.14 DD126 pKa = 3.72 YY127 pKa = 11.35 QSQWTKK133 pKa = 10.38 GFSMQLPATEE143 pKa = 3.96 GAMRR147 pKa = 11.84 YY148 pKa = 4.75 VTKK151 pKa = 10.87 YY152 pKa = 9.31 ILKK155 pKa = 9.8 GSEE158 pKa = 3.78 RR159 pKa = 11.84 DD160 pKa = 3.7 TEE162 pKa = 4.65 IMLCSKK168 pKa = 10.35 KK169 pKa = 10.02 PAIGSSYY176 pKa = 9.66 LTSQMIDD183 pKa = 3.28 YY184 pKa = 9.98 QLQKK188 pKa = 11.07 DD189 pKa = 4.64 EE190 pKa = 4.6 PCYY193 pKa = 8.63 THH195 pKa = 7.38 PGGAVSPLPRR205 pKa = 11.84 YY206 pKa = 8.79 YY207 pKa = 10.34 RR208 pKa = 11.84 KK209 pKa = 10.45 KK210 pKa = 9.33 IWNEE214 pKa = 3.56 STLPHH219 pKa = 6.27 YY220 pKa = 11.11 LEE222 pKa = 4.19 THH224 pKa = 6.36 KK225 pKa = 10.52 NTILEE230 pKa = 4.32 TMEE233 pKa = 4.91 SSIKK237 pKa = 10.66 HH238 pKa = 4.19 NTLIRR243 pKa = 11.84 RR244 pKa = 11.84 YY245 pKa = 8.65 KK246 pKa = 9.7 EE247 pKa = 3.92 KK248 pKa = 10.94 YY249 pKa = 9.55 GSDD252 pKa = 2.86 EE253 pKa = 5.28 GFYY256 pKa = 11.12 DD257 pKa = 3.59 WLNAHH262 pKa = 6.38 YY263 pKa = 10.41 EE264 pKa = 4.02 RR265 pKa = 11.84 DD266 pKa = 3.38 LKK268 pKa = 10.69 IQHH271 pKa = 6.95 KK272 pKa = 10.15 INNSS276 pKa = 3.16
Molecular weight: 32.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.58
IPC2_protein 8.521
IPC_protein 8.39
Toseland 8.843
ProMoST 8.858
Dawson 9.238
Bjellqvist 9.18
Wikipedia 9.414
Rodwell 9.385
Grimsley 9.253
Solomon 9.311
Lehninger 9.268
Nozaki 9.253
DTASelect 9.063
Thurlkill 9.136
EMBOSS 9.37
Sillero 9.326
Patrickios 4.177
IPC_peptide 9.297
IPC2_peptide 7.966
IPC2.peptide.svr19 7.73
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1250
98
524
250.0
28.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.36 ± 0.98
1.12 ± 0.431
5.28 ± 0.429
6.72 ± 0.516
5.28 ± 1.614
5.04 ± 0.857
2.08 ± 0.647
4.96 ± 0.426
6.4 ± 1.123
8.64 ± 0.606
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.4 ± 0.347
6.32 ± 1.129
5.36 ± 0.743
4.96 ± 1.014
5.28 ± 0.384
7.6 ± 1.041
5.12 ± 1.043
5.2 ± 0.818
1.44 ± 0.173
5.44 ± 0.628
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here