Ictalurid herpesvirus 1 (strain Auburn) (IcHV-1) (Channel catfish herpesvirus)
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q00115|VG03_ICHVA Uncharacterized protein ORF3 OS=Ictalurid herpesvirus 1 (strain Auburn) OX=766178 GN=ORF3 PE=4 SV=1
MM1 pKa = 7.55 SATQGPLTEE10 pKa = 4.73 AANGCDD16 pKa = 3.4 DD17 pKa = 3.86 TGLGKK22 pKa = 8.7 TKK24 pKa = 10.33 NHH26 pKa = 6.59 WDD28 pKa = 3.41 GAVPRR33 pKa = 11.84 TTGEE37 pKa = 3.89 PRR39 pKa = 11.84 SLLGEE44 pKa = 3.98 NRR46 pKa = 11.84 ASSVLTTGLGPKK58 pKa = 9.48 PGPVWVDD65 pKa = 2.67 PRR67 pKa = 11.84 IRR69 pKa = 11.84 FHH71 pKa = 6.76 GVSEE75 pKa = 4.36 SLPLTDD81 pKa = 4.8 TPWGPPPSPMDD92 pKa = 3.98 ALGHH96 pKa = 5.8 GVMTPSGLKK105 pKa = 8.78 WCPPPPPKK113 pKa = 10.17 TEE115 pKa = 3.7 SRR117 pKa = 11.84 SFLEE121 pKa = 4.42 LLAMHH126 pKa = 6.51 QKK128 pKa = 10.33 AQADD132 pKa = 3.83 RR133 pKa = 11.84 DD134 pKa = 3.96 DD135 pKa = 4.09 TVTGEE140 pKa = 4.38 TEE142 pKa = 3.79 TTARR146 pKa = 11.84 EE147 pKa = 3.84 EE148 pKa = 4.17 DD149 pKa = 3.51 DD150 pKa = 4.1 VIFVEE155 pKa = 4.56 EE156 pKa = 4.4 TNVNNPDD163 pKa = 3.54 VIEE166 pKa = 4.46 VIEE169 pKa = 4.54 LKK171 pKa = 10.83 DD172 pKa = 3.53 VTEE175 pKa = 4.56 TGHH178 pKa = 5.5 GTLKK182 pKa = 10.03 RR183 pKa = 11.84 RR184 pKa = 11.84 YY185 pKa = 7.82 PMRR188 pKa = 11.84 VRR190 pKa = 11.84 RR191 pKa = 11.84 APKK194 pKa = 9.99 RR195 pKa = 11.84 LVLDD199 pKa = 3.73 EE200 pKa = 4.48 QVVDD204 pKa = 5.49 DD205 pKa = 5.08 YY206 pKa = 11.63 PADD209 pKa = 3.99 SDD211 pKa = 4.93 DD212 pKa = 4.25 DD213 pKa = 3.95 TDD215 pKa = 4.99 AEE217 pKa = 4.56 SDD219 pKa = 3.57 DD220 pKa = 3.93 AMSVLSDD227 pKa = 3.39 TCRR230 pKa = 11.84 TDD232 pKa = 4.67 DD233 pKa = 4.27 DD234 pKa = 4.85 ASSVSSCGSFITDD247 pKa = 3.61 GSGSEE252 pKa = 4.17 EE253 pKa = 4.57 SEE255 pKa = 4.44 DD256 pKa = 3.84 SASDD260 pKa = 3.47 EE261 pKa = 4.41 TDD263 pKa = 4.19 DD264 pKa = 5.59 SDD266 pKa = 5.17 FDD268 pKa = 4.12 TDD270 pKa = 3.53 EE271 pKa = 4.36 LTSEE275 pKa = 4.28 SEE277 pKa = 4.23 EE278 pKa = 4.11 EE279 pKa = 4.06 EE280 pKa = 4.36 SEE282 pKa = 4.39 SEE284 pKa = 4.25 SEE286 pKa = 4.37 SEE288 pKa = 4.35 SEE290 pKa = 4.55 SEE292 pKa = 4.63 SEE294 pKa = 4.76 SEE296 pKa = 4.06
Molecular weight: 32.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.288
IPC2_protein 3.973
IPC_protein 3.973
Toseland 3.77
ProMoST 4.101
Dawson 3.948
Bjellqvist 4.113
Wikipedia 3.846
Rodwell 3.808
Grimsley 3.681
Solomon 3.935
Lehninger 3.897
Nozaki 4.05
DTASelect 4.253
Thurlkill 3.808
EMBOSS 3.859
Sillero 4.088
Patrickios 3.757
IPC_peptide 3.948
IPC2_peptide 4.075
IPC2.peptide.svr19 4.007
Protein with the highest isoelectric point:
>sp|Q00146|VG36_ICHVA Uncharacterized protein ORF36 OS=Ictalurid herpesvirus 1 (strain Auburn) OX=766178 GN=ORF36 PE=4 SV=1
MM1 pKa = 7.28 TPVPPSSLRR10 pKa = 11.84 SIAPLSTVFLFTDD23 pKa = 3.87 NVRR26 pKa = 11.84 SNSWFVCKK34 pKa = 10.2 IVYY37 pKa = 9.81 RR38 pKa = 11.84 CLSPRR43 pKa = 11.84 RR44 pKa = 11.84 IISPSICTAFSCWANHH60 pKa = 5.15 GLRR63 pKa = 11.84 LSSTAAMRR71 pKa = 11.84 RR72 pKa = 11.84 SSSSTFLFMASTRR85 pKa = 11.84 MSSSTFVCFIFVFCVSRR102 pKa = 11.84 TAMVFWSFSEE112 pKa = 4.35 STSLLWCIISSSMRR126 pKa = 11.84 LCFSITSGSTT136 pKa = 2.85
Molecular weight: 15.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.291
IPC2_protein 9.18
IPC_protein 9.97
Toseland 9.984
ProMoST 9.94
Dawson 10.233
Bjellqvist 10.233
Wikipedia 10.452
Rodwell 10.189
Grimsley 10.248
Solomon 10.409
Lehninger 10.379
Nozaki 10.482
DTASelect 10.072
Thurlkill 10.16
EMBOSS 10.452
Sillero 10.321
Patrickios 10.116
IPC_peptide 10.394
IPC2_peptide 10.145
IPC2.peptide.svr19 8.257
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
76
0
76
33924
82
1556
446.4
49.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.464 ± 0.271
1.816 ± 0.149
5.636 ± 0.158
5.769 ± 0.241
4.021 ± 0.182
7.116 ± 0.206
2.273 ± 0.09
5.545 ± 0.175
4.345 ± 0.256
9.114 ± 0.24
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.803 ± 0.112
3.44 ± 0.164
6.143 ± 0.321
2.411 ± 0.165
6.624 ± 0.241
6.367 ± 0.176
7.623 ± 0.498
7.502 ± 0.146
1.197 ± 0.087
2.792 ± 0.14
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here