Methyloceanibacter caenitepidi

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3341 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A8K2A1|A0A0A8K2A1_9RHIZ ABC transporter domain-containing protein OS=Methyloceanibacter caenitepidi OX=1384459 GN=GL4_0671 PE=3 SV=1
MM1 pKa = 7.43KK2 pKa = 9.04HH3 pKa = 4.33TAYY6 pKa = 10.48VGSISTGTLLTEE18 pKa = 4.73DD19 pKa = 4.04LLEE22 pKa = 4.46AFVGEE27 pKa = 4.58LSFLADD33 pKa = 3.84SVDD36 pKa = 3.63HH37 pKa = 6.71PGNRR41 pKa = 11.84QTYY44 pKa = 10.2EE45 pKa = 4.3DD46 pKa = 4.37LCQEE50 pKa = 5.16AIDD53 pKa = 4.81ADD55 pKa = 4.33PEE57 pKa = 4.32SEE59 pKa = 4.23DD60 pKa = 3.51AQEE63 pKa = 4.46ILNSLIDD70 pKa = 4.27ALTEE74 pKa = 3.81LAPPYY79 pKa = 9.96CYY81 pKa = 10.26FGARR85 pKa = 11.84EE86 pKa = 3.89GDD88 pKa = 3.66GADD91 pKa = 3.22FGFWPDD97 pKa = 3.01TDD99 pKa = 5.09SIAEE103 pKa = 4.27LPRR106 pKa = 11.84VSDD109 pKa = 3.93PNEE112 pKa = 3.7VRR114 pKa = 11.84IADD117 pKa = 3.97HH118 pKa = 7.39DD119 pKa = 3.91WLFVNDD125 pKa = 4.6HH126 pKa = 6.22GNITIYY132 pKa = 10.59SGATAQPILEE142 pKa = 4.36LVV144 pKa = 3.37

Molecular weight: 15.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A8K5B1|A0A0A8K5B1_9RHIZ 50S ribosomal protein L25 OS=Methyloceanibacter caenitepidi OX=1384459 GN=rplY PE=3 SV=1
MM1 pKa = 7.39ATRR4 pKa = 11.84TTGKK8 pKa = 10.15KK9 pKa = 8.29RR10 pKa = 11.84TASKK14 pKa = 8.76KK15 pKa = 7.73TPARR19 pKa = 11.84RR20 pKa = 11.84TAARR24 pKa = 11.84RR25 pKa = 11.84TAARR29 pKa = 11.84RR30 pKa = 11.84PAARR34 pKa = 11.84KK35 pKa = 6.38TAARR39 pKa = 11.84KK40 pKa = 6.43TAARR44 pKa = 11.84KK45 pKa = 4.82TTARR49 pKa = 11.84KK50 pKa = 6.62TAARR54 pKa = 11.84KK55 pKa = 6.46TARR58 pKa = 11.84KK59 pKa = 7.64STARR63 pKa = 11.84KK64 pKa = 7.24STARR68 pKa = 11.84KK69 pKa = 6.32TAARR73 pKa = 11.84KK74 pKa = 9.6SPARR78 pKa = 11.84KK79 pKa = 6.81TAARR83 pKa = 11.84KK84 pKa = 6.46TAARR88 pKa = 11.84KK89 pKa = 6.46TARR92 pKa = 11.84KK93 pKa = 7.69STARR97 pKa = 11.84KK98 pKa = 8.3SAARR102 pKa = 11.84KK103 pKa = 6.18TAARR107 pKa = 11.84KK108 pKa = 9.6SPARR112 pKa = 11.84KK113 pKa = 6.81TAARR117 pKa = 11.84KK118 pKa = 6.46TAARR122 pKa = 11.84KK123 pKa = 6.46TARR126 pKa = 11.84KK127 pKa = 7.69STARR131 pKa = 11.84KK132 pKa = 8.3SAARR136 pKa = 11.84KK137 pKa = 6.19TAARR141 pKa = 11.84RR142 pKa = 11.84SPARR146 pKa = 11.84KK147 pKa = 6.76TAARR151 pKa = 11.84KK152 pKa = 6.44TAARR156 pKa = 11.84KK157 pKa = 7.78STARR161 pKa = 11.84KK162 pKa = 7.28STARR166 pKa = 11.84KK167 pKa = 8.27SAARR171 pKa = 11.84KK172 pKa = 7.34STARR176 pKa = 11.84KK177 pKa = 7.28STARR181 pKa = 11.84KK182 pKa = 8.04SAARR186 pKa = 11.84RR187 pKa = 11.84GTARR191 pKa = 11.84KK192 pKa = 9.15SAAKK196 pKa = 10.04KK197 pKa = 9.83SAAKK201 pKa = 10.16KK202 pKa = 9.01SARR205 pKa = 11.84KK206 pKa = 9.62ASARR210 pKa = 11.84KK211 pKa = 9.43APAKK215 pKa = 10.3KK216 pKa = 8.9RR217 pKa = 11.84TAAKK221 pKa = 10.05KK222 pKa = 8.71KK223 pKa = 7.61TAARR227 pKa = 11.84KK228 pKa = 8.37PAVKK232 pKa = 10.26KK233 pKa = 10.5SAAKK237 pKa = 10.24KK238 pKa = 8.48KK239 pKa = 7.79TAARR243 pKa = 11.84KK244 pKa = 6.69TAAKK248 pKa = 8.72KK249 pKa = 8.54TVAKK253 pKa = 10.1KK254 pKa = 9.2KK255 pKa = 8.77TVAKK259 pKa = 10.17KK260 pKa = 9.94AAPKK264 pKa = 9.94KK265 pKa = 10.07AAAKK269 pKa = 9.86KK270 pKa = 9.67AAPKK274 pKa = 9.99KK275 pKa = 9.89AAPKK279 pKa = 9.63KK280 pKa = 9.25AAPKK284 pKa = 9.99KK285 pKa = 10.0VEE287 pKa = 4.22TKK289 pKa = 10.18KK290 pKa = 10.91VEE292 pKa = 4.31PKK294 pKa = 9.78TVAPTPKK301 pKa = 9.53PVASPAPRR309 pKa = 11.84PPVAQPAQRR318 pKa = 11.84PPLAQPSPSPARR330 pKa = 11.84PSSPGAGGTPPRR342 pKa = 11.84HH343 pKa = 5.71VSPGSAIPGYY353 pKa = 9.48QPPRR357 pKa = 11.84PPMPGSTPGPTPTPMPYY374 pKa = 9.6RR375 pKa = 11.84PSWARR380 pKa = 11.84DD381 pKa = 3.24DD382 pKa = 4.42DD383 pKa = 4.0

Molecular weight: 40.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3341

0

3341

996689

37

3557

298.3

32.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.027 ± 0.064

0.874 ± 0.015

6.077 ± 0.045

6.214 ± 0.044

3.724 ± 0.031

8.491 ± 0.053

1.964 ± 0.021

4.944 ± 0.033

3.811 ± 0.037

9.882 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.335 ± 0.023

2.68 ± 0.029

5.286 ± 0.031

3.065 ± 0.024

6.612 ± 0.044

5.372 ± 0.03

5.421 ± 0.036

7.472 ± 0.041

1.339 ± 0.02

2.41 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski