Pseudoalteromonas luteoviolacea DSM 6061

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Pseudoalteromonadaceae; Pseudoalteromonas; Pseudoalteromonas luteoviolacea

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5008 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A166WEY1|A0A166WEY1_9GAMM 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsC OS=Pseudoalteromonas luteoviolacea DSM 6061 OX=1365250 GN=N475_16615 PE=3 SV=1
MM1 pKa = 7.68KK2 pKa = 10.07KK3 pKa = 10.01YY4 pKa = 10.3CLAAALSLACLTPAAHH20 pKa = 7.58ADD22 pKa = 3.9TLLGLYY28 pKa = 10.13LGAEE32 pKa = 5.15GWQSEE37 pKa = 4.64PEE39 pKa = 3.94GSFAEE44 pKa = 4.34KK45 pKa = 11.05GNLQEE50 pKa = 5.94FNFEE54 pKa = 4.31DD55 pKa = 3.49EE56 pKa = 5.19TYY58 pKa = 9.04TSLYY62 pKa = 10.72ASLEE66 pKa = 4.27HH67 pKa = 6.65PVPLVPNVKK76 pKa = 10.26LKK78 pKa = 9.44YY79 pKa = 9.21TEE81 pKa = 5.02LEE83 pKa = 4.1LTGNTTLTDD92 pKa = 3.34TFEE95 pKa = 4.69FGGSNFTVGTTANTVADD112 pKa = 5.03LSHH115 pKa = 7.35IDD117 pKa = 3.53YY118 pKa = 10.64ILYY121 pKa = 10.73YY122 pKa = 10.56EE123 pKa = 5.41LFDD126 pKa = 4.26NDD128 pKa = 4.13LVSFDD133 pKa = 4.16FGINAKK139 pKa = 10.08QFDD142 pKa = 4.32ADD144 pKa = 3.76VTVTGEE150 pKa = 3.98VNGAQVTEE158 pKa = 4.29TVDD161 pKa = 3.46FSGFVPLGYY170 pKa = 10.44LRR172 pKa = 11.84AEE174 pKa = 4.15VGLPLTGLSVFAEE187 pKa = 4.93GSLLAIDD194 pKa = 4.45DD195 pKa = 4.68SKK197 pKa = 11.29IQDD200 pKa = 3.84YY201 pKa = 10.95QIGIAWEE208 pKa = 4.8FIDD211 pKa = 4.93NLAVDD216 pKa = 3.94VALRR220 pKa = 11.84AGYY223 pKa = 9.95RR224 pKa = 11.84SLVLEE229 pKa = 5.21LDD231 pKa = 5.12DD232 pKa = 6.18IDD234 pKa = 6.37DD235 pKa = 4.33FDD237 pKa = 6.62SDD239 pKa = 4.07IDD241 pKa = 3.77ASGPFAGLQVHH252 pKa = 6.57FF253 pKa = 4.92

Molecular weight: 27.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A162ACJ6|A0A162ACJ6_9GAMM Uncharacterized protein OS=Pseudoalteromonas luteoviolacea DSM 6061 OX=1365250 GN=N475_06795 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.97RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37NGRR28 pKa = 11.84KK29 pKa = 8.96VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.11GRR39 pKa = 11.84KK40 pKa = 8.16EE41 pKa = 3.63LSAA44 pKa = 4.99

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5008

0

5008

1708250

30

6713

341.1

38.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.357 ± 0.036

1.059 ± 0.012

5.48 ± 0.032

6.279 ± 0.033

4.288 ± 0.024

6.463 ± 0.037

2.362 ± 0.017

6.223 ± 0.025

5.648 ± 0.035

10.313 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.279 ± 0.016

4.681 ± 0.026

3.68 ± 0.02

4.959 ± 0.036

4.101 ± 0.022

7.075 ± 0.029

5.411 ± 0.031

6.831 ± 0.027

1.192 ± 0.012

3.319 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski