Streptococcus satellite phage Javan469
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 24 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZPS1|A0A4D5ZPS1_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan469 OX=2558717 GN=JavanS469_0013 PE=4 SV=1
MM1 pKa = 7.72 AKK3 pKa = 10.2 NSPQDD8 pKa = 3.86 LINRR12 pKa = 11.84 EE13 pKa = 3.91 IFSTNLNMLMAKK25 pKa = 10.2 KK26 pKa = 10.1 NIKK29 pKa = 10.21 QIDD32 pKa = 3.46 IHH34 pKa = 6.66 NKK36 pKa = 9.74 LGIPKK41 pKa = 8.5 STITGYY47 pKa = 10.91 VKK49 pKa = 10.57 GRR51 pKa = 11.84 SLPTAGNVQKK61 pKa = 10.8 LADD64 pKa = 3.93 FFGVLKK70 pKa = 10.84 SDD72 pKa = 3.19 IDD74 pKa = 3.86 PRR76 pKa = 11.84 FDD78 pKa = 4.03 SNNIEE83 pKa = 4.29 TNSNIIPSTLQKK95 pKa = 9.47 VTSTLSQLEE104 pKa = 4.51 HH105 pKa = 6.54 KK106 pKa = 10.36 RR107 pKa = 11.84 QLNVLDD113 pKa = 3.87 YY114 pKa = 11.66 AEE116 pKa = 4.47 TQLEE120 pKa = 4.14 QQNTVEE126 pKa = 4.75 EE127 pKa = 4.39 PQATYY132 pKa = 8.87 YY133 pKa = 9.48 TYY135 pKa = 11.16 NYY137 pKa = 9.02 YY138 pKa = 11.1 DD139 pKa = 3.61 HH140 pKa = 7.34 AASAGTGQYY149 pKa = 11.03 LNDD152 pKa = 3.69 VQVEE156 pKa = 4.58 TIEE159 pKa = 5.25 LPVDD163 pKa = 3.45 YY164 pKa = 10.6 DD165 pKa = 3.94 ADD167 pKa = 3.98 FVIPVYY173 pKa = 10.34 GDD175 pKa = 3.3 SMEE178 pKa = 4.56 PEE180 pKa = 4.48 YY181 pKa = 11.08 HH182 pKa = 6.24 SGDD185 pKa = 3.53 YY186 pKa = 11.17 VFIKK190 pKa = 10.62 LSINLSDD197 pKa = 3.52 GDD199 pKa = 3.54 IGVFEE204 pKa = 4.94 YY205 pKa = 11.34 YY206 pKa = 10.64 GDD208 pKa = 4.18 AYY210 pKa = 9.96 IKK212 pKa = 10.44 QLVINDD218 pKa = 3.53 SGAFLHH224 pKa = 6.49 SLNDD228 pKa = 3.42 KK229 pKa = 10.89 YY230 pKa = 11.69 DD231 pKa = 5.24 DD232 pKa = 3.68 ILIDD236 pKa = 3.7 RR237 pKa = 11.84 DD238 pKa = 3.23 SDD240 pKa = 3.49 FRR242 pKa = 11.84 IIGEE246 pKa = 4.32 VIGSFTSKK254 pKa = 10.46 SS255 pKa = 3.33
Molecular weight: 28.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.421
IPC2_protein 4.482
IPC_protein 4.444
Toseland 4.266
ProMoST 4.584
Dawson 4.431
Bjellqvist 4.571
Wikipedia 4.342
Rodwell 4.279
Grimsley 4.177
Solomon 4.418
Lehninger 4.38
Nozaki 4.533
DTASelect 4.774
Thurlkill 4.291
EMBOSS 4.355
Sillero 4.571
Patrickios 3.478
IPC_peptide 4.418
IPC2_peptide 4.558
IPC2.peptide.svr19 4.5
Protein with the highest isoelectric point:
>tr|A0A4D5ZNR6|A0A4D5ZNR6_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan469 OX=2558717 GN=JavanS469_0012 PE=4 SV=1
MM1 pKa = 7.57 SGGGAYY7 pKa = 9.43 VCKK10 pKa = 10.2 SQKK13 pKa = 9.01 PKK15 pKa = 9.91 EE16 pKa = 4.06 RR17 pKa = 11.84 RR18 pKa = 11.84 RR19 pKa = 11.84 QGLSVYY25 pKa = 7.65 EE26 pKa = 4.27 TLTLMIAFGTLIVAIMNNKK45 pKa = 8.89 NKK47 pKa = 10.56
Molecular weight: 5.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.214
IPC2_protein 9.589
IPC_protein 9.692
Toseland 10.365
ProMoST 10.058
Dawson 10.496
Bjellqvist 10.131
Wikipedia 10.628
Rodwell 11.082
Grimsley 10.54
Solomon 10.54
Lehninger 10.526
Nozaki 10.35
DTASelect 10.116
Thurlkill 10.365
EMBOSS 10.745
Sillero 10.409
Patrickios 10.906
IPC_peptide 10.54
IPC2_peptide 8.873
IPC2.peptide.svr19 8.516
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
24
0
24
3545
47
464
147.7
17.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.444 ± 0.28
0.846 ± 0.248
6.065 ± 0.44
7.109 ± 0.508
3.808 ± 0.262
5.106 ± 0.29
1.693 ± 0.218
6.488 ± 0.36
9.111 ± 0.478
9.647 ± 0.62
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.228 ± 0.264
5.388 ± 0.343
3.385 ± 0.564
4.908 ± 0.242
5.19 ± 0.332
5.557 ± 0.319
6.206 ± 0.305
5.416 ± 0.265
1.044 ± 0.217
5.36 ± 0.269
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here