Streptococcus phage Javan477

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AI54|A0A4D6AI54_9CAUD Holin OS=Streptococcus phage Javan477 OX=2548194 GN=Javan477_0052 PE=4 SV=1
MM1 pKa = 7.96AILEE5 pKa = 4.13IKK7 pKa = 8.59ITNDD11 pKa = 2.92AGEE14 pKa = 4.42KK15 pKa = 9.49VVKK18 pKa = 9.51EE19 pKa = 4.32CKK21 pKa = 10.09SLTVRR26 pKa = 11.84DD27 pKa = 3.73YY28 pKa = 11.53RR29 pKa = 11.84DD30 pKa = 3.49YY31 pKa = 11.62LVLQQEE37 pKa = 4.97LSDD40 pKa = 3.94SDD42 pKa = 3.66APEE45 pKa = 3.86YY46 pKa = 11.26AKK48 pKa = 10.62LDD50 pKa = 3.64RR51 pKa = 11.84QLEE54 pKa = 4.69FIVGLFDD61 pKa = 4.32GLTVDD66 pKa = 4.54MMYY69 pKa = 11.09DD70 pKa = 3.34KK71 pKa = 11.68LNMYY75 pKa = 8.94EE76 pKa = 4.49LNNILADD83 pKa = 3.82VYY85 pKa = 11.36VKK87 pKa = 10.88LIGGDD92 pKa = 3.47PDD94 pKa = 4.28DD95 pKa = 5.28PKK97 pKa = 11.84DD98 pKa = 3.6NAA100 pKa = 4.25

Molecular weight: 11.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AG68|A0A4D6AG68_9CAUD Uncharacterized protein OS=Streptococcus phage Javan477 OX=2548194 GN=Javan477_0021 PE=4 SV=1
MM1 pKa = 7.57PRR3 pKa = 11.84YY4 pKa = 9.53LKK6 pKa = 10.81YY7 pKa = 10.56ILIIVALLNGKK18 pKa = 8.71MSLNVIAKK26 pKa = 9.69RR27 pKa = 11.84KK28 pKa = 8.98GYY30 pKa = 10.4EE31 pKa = 3.8YY32 pKa = 10.59DD33 pKa = 3.65RR34 pKa = 11.84KK35 pKa = 9.41STKK38 pKa = 9.77CSYY41 pKa = 10.64LFSHH45 pKa = 7.67PIFNSIFMM53 pKa = 5.12

Molecular weight: 6.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

11558

38

1366

210.1

23.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.766 ± 0.628

0.51 ± 0.096

6.42 ± 0.313

7.951 ± 0.396

3.98 ± 0.218

5.961 ± 0.335

1.497 ± 0.151

7.147 ± 0.261

9.465 ± 0.303

8.929 ± 0.346

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.492 ± 0.138

5.607 ± 0.242

2.457 ± 0.128

4.136 ± 0.231

4.092 ± 0.23

6.402 ± 0.263

5.684 ± 0.312

5.823 ± 0.278

1.073 ± 0.118

3.608 ± 0.301
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski