Meleagris gallopavo (Wild turkey)
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 16533 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G1NFC5|G1NFC5_MELGA Tripartite motif containing 55 OS=Meleagris gallopavo OX=9103 GN=TRIM55 PE=4 SV=2
MM1 pKa = 7.52 AVFHH5 pKa = 7.31 DD6 pKa = 4.18 EE7 pKa = 4.41 VEE9 pKa = 4.39 IEE11 pKa = 4.0 DD12 pKa = 3.93 FEE14 pKa = 4.87 YY15 pKa = 11.07 DD16 pKa = 4.0 EE17 pKa = 4.26 EE18 pKa = 4.86 TEE20 pKa = 4.5 TYY22 pKa = 10.31 SYY24 pKa = 10.49 PCPCGDD30 pKa = 3.17 RR31 pKa = 11.84 FLITRR36 pKa = 11.84 EE37 pKa = 3.86 DD38 pKa = 3.83 LEE40 pKa = 4.55 NGEE43 pKa = 5.51 DD44 pKa = 3.76 VATCPSCSLILRR56 pKa = 11.84 VIYY59 pKa = 10.21 DD60 pKa = 3.18 QEE62 pKa = 4.01 QFMRR66 pKa = 11.84 DD67 pKa = 3.31 EE68 pKa = 4.88 VIAEE72 pKa = 3.93 PLTNKK77 pKa = 10.13 EE78 pKa = 4.0 LVKK81 pKa = 10.96 CC82 pKa = 4.18
Molecular weight: 9.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.811
IPC2_protein 3.948
IPC_protein 3.872
Toseland 3.694
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.986
Wikipedia 3.719
Rodwell 3.706
Grimsley 3.605
Solomon 3.808
Lehninger 3.77
Nozaki 3.948
DTASelect 4.075
Thurlkill 3.719
EMBOSS 3.732
Sillero 3.973
Patrickios 0.604
IPC_peptide 3.821
IPC2_peptide 3.961
IPC2.peptide.svr19 3.889
Protein with the highest isoelectric point:
>tr|H9H0M2|H9H0M2_MELGA Uncharacterized protein OS=Meleagris gallopavo OX=9103 PE=4 SV=1
KKK2 pKa = 9.31 RR3 pKa = 11.84 SRR5 pKa = 11.84 SRR7 pKa = 11.84 SRR9 pKa = 11.84 SRR11 pKa = 11.84 SRR13 pKa = 11.84 GRR15 pKa = 11.84 RR16 pKa = 11.84 YYY18 pKa = 9.62 RR19 pKa = 11.84 SRR21 pKa = 11.84 SRR23 pKa = 11.84 SRR25 pKa = 11.84 GRR27 pKa = 11.84 RR28 pKa = 11.84 SRR30 pKa = 11.84 SASYYY35 pKa = 9.96 RR36 pKa = 11.84 SRR38 pKa = 11.84 SVSPRR43 pKa = 11.84 RR44 pKa = 11.84 SRR46 pKa = 11.84 SISPRR51 pKa = 11.84 RR52 pKa = 11.84 SRR54 pKa = 11.84 SGSLKKK60 pKa = 9.79 SRR62 pKa = 11.84 SRR64 pKa = 11.84 SRR66 pKa = 11.84 SRR68 pKa = 11.84 SRR70 pKa = 11.84 SRR72 pKa = 11.84 SVTWPRR78 pKa = 11.84 SSRR81 pKa = 11.84 SKKK84 pKa = 10.75 RR85 pKa = 11.84 SPSPKKK91 pKa = 9.03 SRR93 pKa = 11.84 SPSGSP
Molecular weight: 11.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.525
IPC2_protein 11.462
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.588
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.296
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.252
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14164
2369
16533
8776270
19
8404
530.8
59.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.556 ± 0.019
2.301 ± 0.015
4.972 ± 0.012
7.227 ± 0.026
3.808 ± 0.014
5.947 ± 0.025
2.534 ± 0.009
4.869 ± 0.015
6.316 ± 0.023
9.708 ± 0.022
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.213 ± 0.008
4.063 ± 0.012
5.392 ± 0.022
4.758 ± 0.018
5.263 ± 0.013
8.385 ± 0.023
5.372 ± 0.014
6.202 ± 0.015
1.18 ± 0.007
2.902 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here