Morus notabilis
Average proteome isoelectric point is 6.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 26760 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W9RVP0|W9RVP0_9ROSA Zinc finger A20 and AN1 domain-containing stress-associated protein 5 OS=Morus notabilis OX=981085 GN=L484_008234 PE=4 SV=1
MM1 pKa = 7.5 ASVATSEE8 pKa = 4.01 KK9 pKa = 10.58 CVFIDD14 pKa = 4.53 GDD16 pKa = 4.03 DD17 pKa = 3.64 GFEE20 pKa = 4.46 AVDD23 pKa = 3.28 IDD25 pKa = 4.38 SAFLMSLMEE34 pKa = 4.31 EE35 pKa = 3.91 LHH37 pKa = 6.63 DD38 pKa = 3.75 QVEE41 pKa = 4.68 VTDD44 pKa = 3.87 EE45 pKa = 4.02 EE46 pKa = 4.37 RR47 pKa = 11.84 LKK49 pKa = 11.2 SVIQSLEE56 pKa = 3.79 AEE58 pKa = 4.69 INSSTAVDD66 pKa = 4.4 LDD68 pKa = 3.53 HH69 pKa = 7.67 DD70 pKa = 5.14 SIYY73 pKa = 10.8 MMEE76 pKa = 4.93 PDD78 pKa = 4.11 HH79 pKa = 7.19 DD80 pKa = 4.57 RR81 pKa = 11.84 SISDD85 pKa = 3.8 RR86 pKa = 11.84 EE87 pKa = 4.0 DD88 pKa = 3.23 SQACSLGPMDD98 pKa = 5.38 DD99 pKa = 3.5 QDD101 pKa = 4.25 FSVSFDD107 pKa = 3.75 DD108 pKa = 5.79 LDD110 pKa = 3.81 MNRR113 pKa = 11.84 LINMEE118 pKa = 4.69 AVPCSPNHH126 pKa = 5.6 WDD128 pKa = 3.64 MYY130 pKa = 10.75 PFGDD134 pKa = 3.99 HH135 pKa = 7.38 DD136 pKa = 4.66 QITSNDD142 pKa = 3.17 YY143 pKa = 10.8 SADD146 pKa = 3.05 HH147 pKa = 5.6 FTYY150 pKa = 10.74 NRR152 pKa = 11.84 GVVLDD157 pKa = 3.44 QVYY160 pKa = 10.36 GYY162 pKa = 11.16 NSLWQEE168 pKa = 4.13 TTHH171 pKa = 7.02 DD172 pKa = 3.7 SVMYY176 pKa = 9.74 EE177 pKa = 3.79
Molecular weight: 20.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.702
IPC2_protein 3.719
IPC_protein 3.745
Toseland 3.516
ProMoST 3.897
Dawson 3.745
Bjellqvist 3.923
Wikipedia 3.694
Rodwell 3.567
Grimsley 3.427
Solomon 3.732
Lehninger 3.694
Nozaki 3.859
DTASelect 4.113
Thurlkill 3.579
EMBOSS 3.694
Sillero 3.872
Patrickios 0.871
IPC_peptide 3.732
IPC2_peptide 3.846
IPC2.peptide.svr19 3.786
Protein with the highest isoelectric point:
>tr|W9R0B7|W9R0B7_9ROSA Tyrosyl-DNA phosphodiesterase 1 OS=Morus notabilis OX=981085 GN=L484_014813 PE=3 SV=1
MM1 pKa = 7.49 AAAISAPQKK10 pKa = 10.66 LRR12 pKa = 11.84 IANHH16 pKa = 5.67 QPLLPNLSFSSHH28 pKa = 3.57 TRR30 pKa = 11.84 LRR32 pKa = 11.84 RR33 pKa = 11.84 KK34 pKa = 9.88 SKK36 pKa = 10.59 LGPAPKK42 pKa = 9.77 RR43 pKa = 11.84 WISAQGKK50 pKa = 8.66 KK51 pKa = 9.59 RR52 pKa = 11.84 EE53 pKa = 3.9 RR54 pKa = 11.84 RR55 pKa = 11.84 IKK57 pKa = 10.68 NVAKK61 pKa = 10.27 PLL63 pKa = 3.78
Molecular weight: 7.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.356
IPC2_protein 10.774
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.34
Grimsley 12.544
Solomon 12.983
Lehninger 12.881
Nozaki 12.501
DTASelect 12.486
Thurlkill 12.501
EMBOSS 12.998
Sillero 12.501
Patrickios 12.076
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.036
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
26760
0
26760
9697202
50
5097
362.4
40.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.834 ± 0.014
1.835 ± 0.008
5.296 ± 0.012
6.538 ± 0.016
4.246 ± 0.011
6.602 ± 0.016
2.381 ± 0.008
5.179 ± 0.011
6.046 ± 0.015
9.683 ± 0.022
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.328 ± 0.006
4.412 ± 0.01
4.934 ± 0.017
3.553 ± 0.011
5.622 ± 0.013
9.07 ± 0.02
4.852 ± 0.01
6.586 ± 0.011
1.299 ± 0.005
2.704 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here