Arthrobacter sp. YC-RL1
Average proteome isoelectric point is 6.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3439 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0H0ZTS0|A0A0H0ZTS0_9MICC Phosphomannomutase OS=Arthrobacter sp. YC-RL1 OX=1652545 GN=AA310_04950 PE=3 SV=1
MM1 pKa = 7.04 YY2 pKa = 10.23 RR3 pKa = 11.84 LSATAAALALGLTACAPTSGEE24 pKa = 4.13 DD25 pKa = 3.95 GEE27 pKa = 5.62 GNAADD32 pKa = 3.66 ATKK35 pKa = 10.52 TEE37 pKa = 4.35 VPALQQYY44 pKa = 7.4 YY45 pKa = 7.6 TQQVDD50 pKa = 3.25 WEE52 pKa = 4.66 KK53 pKa = 10.98 CGSTIEE59 pKa = 4.33 CADD62 pKa = 3.28 IEE64 pKa = 4.71 VPLDD68 pKa = 3.3 YY69 pKa = 10.77 AAPEE73 pKa = 4.24 KK74 pKa = 10.82 DD75 pKa = 4.06 SIMLALNRR83 pKa = 11.84 RR84 pKa = 11.84 AVDD87 pKa = 4.0 GAQGNLLVNPGGPGGSGLDD106 pKa = 3.36 MVAEE110 pKa = 4.64 SVQLMLSNDD119 pKa = 3.32 LQRR122 pKa = 11.84 AYY124 pKa = 10.94 NIIGFDD130 pKa = 3.55 PRR132 pKa = 11.84 GVGQSTPVTCQTDD145 pKa = 3.62 KK146 pKa = 11.34 EE147 pKa = 4.15 IDD149 pKa = 3.18 ASRR152 pKa = 11.84 QEE154 pKa = 4.31 NLKK157 pKa = 10.64 AWDD160 pKa = 3.62 PADD163 pKa = 4.3 RR164 pKa = 11.84 DD165 pKa = 3.65 EE166 pKa = 5.27 VEE168 pKa = 5.55 ADD170 pKa = 3.34 TEE172 pKa = 5.23 DD173 pKa = 3.87 YY174 pKa = 11.5 AKK176 pKa = 10.87 DD177 pKa = 3.5 CAEE180 pKa = 4.04 NTGDD184 pKa = 4.78 LLGHH188 pKa = 6.49 VDD190 pKa = 3.68 TVSAAKK196 pKa = 10.85 DD197 pKa = 3.23 MDD199 pKa = 4.35 IIRR202 pKa = 11.84 AVLGDD207 pKa = 3.63 TQLDD211 pKa = 3.92 YY212 pKa = 11.55 LGYY215 pKa = 10.5 SYY217 pKa = 10.66 GTFLGSTYY225 pKa = 11.39 ADD227 pKa = 4.32 LFPQKK232 pKa = 10.25 VGRR235 pKa = 11.84 FVLDD239 pKa = 3.52 GAMDD243 pKa = 4.77 PLATAQQMTLAQAQGFEE260 pKa = 4.34 GEE262 pKa = 3.71 IDD264 pKa = 3.4 AWLQNCLEE272 pKa = 4.5 GEE274 pKa = 4.21 NCPFTGNLDD283 pKa = 3.59 EE284 pKa = 6.57 AKK286 pKa = 9.12 TQLQDD291 pKa = 3.04 FFARR295 pKa = 11.84 IEE297 pKa = 4.06 EE298 pKa = 4.21 QPLEE302 pKa = 4.32 SSDD305 pKa = 3.16 GRR307 pKa = 11.84 TVPIIDD313 pKa = 5.43 FINGFIIPLYY323 pKa = 10.99 DD324 pKa = 4.12 DD325 pKa = 4.32 MNWPYY330 pKa = 9.76 LTQGMAAAMQGDD342 pKa = 4.33 VDD344 pKa = 4.12 QILYY348 pKa = 10.61 FSDD351 pKa = 4.25 LSADD355 pKa = 3.9 RR356 pKa = 11.84 QEE358 pKa = 4.58 DD359 pKa = 3.48 GSYY362 pKa = 11.23 GSNSTDD368 pKa = 2.62 AFTAVNCLDD377 pKa = 3.65 RR378 pKa = 11.84 PMDD381 pKa = 4.01 ASEE384 pKa = 3.96 QAMEE388 pKa = 4.56 ADD390 pKa = 3.36 AKK392 pKa = 10.86 ALEE395 pKa = 4.49 EE396 pKa = 4.14 ASPTIGKK403 pKa = 9.11 YY404 pKa = 9.66 LAYY407 pKa = 10.5 GEE409 pKa = 4.39 LTCDD413 pKa = 2.57 KK414 pKa = 9.76 WSYY417 pKa = 10.5 KK418 pKa = 9.91 ATGQPEE424 pKa = 4.37 SLDD427 pKa = 3.66 APGSNQILVVGTTGDD442 pKa = 3.59 PATPYY447 pKa = 10.75 AWSQSLAEE455 pKa = 4.06 QLQNATLLTYY465 pKa = 10.06 EE466 pKa = 4.37 GHH468 pKa = 6.0 GHH470 pKa = 4.9 TAYY473 pKa = 10.3 GRR475 pKa = 11.84 SNQCITDD482 pKa = 3.41 AVDD485 pKa = 4.58 GYY487 pKa = 11.15 LIDD490 pKa = 4.31 GKK492 pKa = 10.83 VPEE495 pKa = 5.32 AGTVCC500 pKa = 4.44
Molecular weight: 53.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.711
IPC2_protein 3.834
IPC_protein 3.859
Toseland 3.63
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.77
Rodwell 3.681
Grimsley 3.541
Solomon 3.834
Lehninger 3.795
Nozaki 3.948
DTASelect 4.19
Thurlkill 3.681
EMBOSS 3.783
Sillero 3.973
Patrickios 1.024
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.855
Protein with the highest isoelectric point:
>tr|A0A0H0ZND4|A0A0H0ZND4_9MICC TfoX_N domain-containing protein OS=Arthrobacter sp. YC-RL1 OX=1652545 GN=AA310_09315 PE=4 SV=1
MM1 pKa = 7.42 DD2 pKa = 3.12 QQMRR6 pKa = 11.84 RR7 pKa = 11.84 NPTRR11 pKa = 11.84 TRR13 pKa = 11.84 RR14 pKa = 11.84 HH15 pKa = 5.12 NRR17 pKa = 11.84 PNRR20 pKa = 11.84 QRR22 pKa = 11.84 ATTQQRR28 pKa = 11.84 RR29 pKa = 11.84 QQRR32 pKa = 11.84 NQKK35 pKa = 9.18 RR36 pKa = 11.84 RR37 pKa = 11.84 QLSTTQPRR45 pKa = 11.84 RR46 pKa = 11.84 NAVEE50 pKa = 3.84 NQPAKK55 pKa = 9.58 HH56 pKa = 5.91 HH57 pKa = 6.11 PNRR60 pKa = 11.84 RR61 pKa = 11.84 TLRR64 pKa = 11.84 QRR66 pKa = 11.84 PTRR69 pKa = 11.84 SRR71 pKa = 11.84 HH72 pKa = 5.65 RR73 pKa = 11.84 LDD75 pKa = 3.36 NPKK78 pKa = 10.09 LVHH81 pKa = 6.24 QSRR84 pKa = 11.84 TTHH87 pKa = 4.36 QRR89 pKa = 11.84 TRR91 pKa = 11.84 NRR93 pKa = 11.84 THH95 pKa = 7.41 RR96 pKa = 11.84 PPSQPRR102 pKa = 11.84 KK103 pKa = 10.07 DD104 pKa = 3.45 RR105 pKa = 11.84 TDD107 pKa = 3.35 RR108 pKa = 11.84 ATNAHH113 pKa = 6.59 ASTRR117 pKa = 11.84 ALATRR122 pKa = 11.84 KK123 pKa = 9.56 RR124 pKa = 3.68
Molecular weight: 15.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.415
IPC2_protein 10.891
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.13
Rodwell 12.223
Grimsley 12.691
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 11.945
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.136
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3439
0
3439
1069183
41
2163
310.9
33.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.529 ± 0.06
0.64 ± 0.011
5.454 ± 0.037
5.995 ± 0.045
3.332 ± 0.029
8.545 ± 0.04
2.114 ± 0.021
4.867 ± 0.029
3.157 ± 0.032
10.526 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.262 ± 0.018
2.681 ± 0.017
4.858 ± 0.027
3.669 ± 0.024
6.137 ± 0.038
6.107 ± 0.029
5.7 ± 0.037
7.872 ± 0.038
1.391 ± 0.016
2.162 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here