Actinoplanes philippinensis
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9412 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I2IMI6|A0A1I2IMI6_9ACTN Uncharacterized protein OS=Actinoplanes philippinensis OX=35752 GN=SAMN05421541_110107 PE=4 SV=1
MM1 pKa = 7.47 GVQDD5 pKa = 4.45 EE6 pKa = 5.42 PIDD9 pKa = 4.19 PFNGDD14 pKa = 3.56 PTDD17 pKa = 3.89 PAAGLDD23 pKa = 4.1 DD24 pKa = 4.92 LNEE27 pKa = 4.47 DD28 pKa = 4.32 AEE30 pKa = 4.54 SEE32 pKa = 4.14 PLTEE36 pKa = 6.16 DD37 pKa = 2.91 EE38 pKa = 4.55 RR39 pKa = 11.84 QDD41 pKa = 3.73 VLEE44 pKa = 5.08 DD45 pKa = 3.7 LSDD48 pKa = 4.04 LEE50 pKa = 4.86 IYY52 pKa = 9.98 QALLSPTGIRR62 pKa = 11.84 GLVIEE67 pKa = 5.58 CEE69 pKa = 4.47 DD70 pKa = 3.68 CHH72 pKa = 6.56 EE73 pKa = 4.0 PHH75 pKa = 6.71 YY76 pKa = 10.76 FDD78 pKa = 4.51 WDD80 pKa = 3.86 LLRR83 pKa = 11.84 GNLRR87 pKa = 11.84 HH88 pKa = 6.15 LLSSGRR94 pKa = 11.84 PRR96 pKa = 11.84 VHH98 pKa = 6.65 EE99 pKa = 4.06 PAYY102 pKa = 10.8 DD103 pKa = 4.24 PDD105 pKa = 4.78 PDD107 pKa = 5.1 HH108 pKa = 6.67 YY109 pKa = 10.69 VTWEE113 pKa = 3.91 YY114 pKa = 11.54 ARR116 pKa = 11.84 GYY118 pKa = 10.77 ADD120 pKa = 3.94 GVHH123 pKa = 6.44 DD124 pKa = 4.42 TLTEE128 pKa = 4.31 GSDD131 pKa = 4.06 DD132 pKa = 3.81 EE133 pKa = 5.14 APP135 pKa = 3.58
Molecular weight: 15.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.735
IPC2_protein 3.821
IPC_protein 3.821
Toseland 3.617
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.745
Rodwell 3.656
Grimsley 3.528
Solomon 3.808
Lehninger 3.757
Nozaki 3.923
DTASelect 4.151
Thurlkill 3.668
EMBOSS 3.757
Sillero 3.948
Patrickios 1.888
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.833
Protein with the highest isoelectric point:
>tr|A0A1I2JG82|A0A1I2JG82_9ACTN Pantothenate synthetase OS=Actinoplanes philippinensis OX=35752 GN=panC PE=3 SV=1
MM1 pKa = 7.28 GSVVKK6 pKa = 10.49 KK7 pKa = 9.45 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.48 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.87 KK16 pKa = 9.7 HH17 pKa = 5.84 RR18 pKa = 11.84 KK19 pKa = 8.7 LLRR22 pKa = 11.84 KK23 pKa = 7.84 TRR25 pKa = 11.84 VQRR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 LGKK33 pKa = 10.04
Molecular weight: 4.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.184
IPC_protein 12.749
Toseland 12.925
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.735
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.457
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9412
0
9412
3122312
29
7909
331.7
35.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.094 ± 0.041
0.704 ± 0.008
6.06 ± 0.019
5.0 ± 0.023
2.751 ± 0.015
9.405 ± 0.024
2.124 ± 0.013
3.619 ± 0.018
1.767 ± 0.02
10.282 ± 0.034
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.682 ± 0.011
1.88 ± 0.017
6.177 ± 0.028
2.664 ± 0.015
8.114 ± 0.03
5.06 ± 0.021
6.374 ± 0.027
8.639 ± 0.025
1.586 ± 0.011
2.019 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here