Streptocarpus flower break virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Virgaviridae; Tobamovirus

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q1XF43|Q1XF43_9VIRU Movement protein OS=Streptocarpus flower break virus OX=335187 GN=p31 PE=3 SV=1
MM1 pKa = 7.47MNLCSASLSQIFQTQAARR19 pKa = 11.84DD20 pKa = 3.66AVRR23 pKa = 11.84EE24 pKa = 4.02QFGNTLRR31 pKa = 11.84SVVTQTTRR39 pKa = 11.84FPEE42 pKa = 4.29AGFQVYY48 pKa = 10.48INDD51 pKa = 4.07AALKK55 pKa = 9.17PLWEE59 pKa = 4.38NLLKK63 pKa = 11.11SFDD66 pKa = 3.61TKK68 pKa = 11.27NRR70 pKa = 11.84ILEE73 pKa = 4.43TEE75 pKa = 4.12EE76 pKa = 3.49EE77 pKa = 4.54TRR79 pKa = 11.84PSTAEE84 pKa = 3.77VLNATQRR91 pKa = 11.84VDD93 pKa = 4.05DD94 pKa = 3.98STTAIRR100 pKa = 11.84GALQRR105 pKa = 11.84LSDD108 pKa = 3.73EE109 pKa = 4.6LNRR112 pKa = 11.84GSGYY116 pKa = 8.26MNRR119 pKa = 11.84TSFEE123 pKa = 4.41TILLWSAATAKK134 pKa = 10.65

Molecular weight: 15.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q1XF44|Q1XF44_9VIRU Methyltransferase/RNA helicase OS=Streptocarpus flower break virus OX=335187 GN=55 PE=4 SV=1
MM1 pKa = 7.7AIVLSKK7 pKa = 10.73PKK9 pKa = 10.41VSEE12 pKa = 3.86FLNLTKK18 pKa = 10.55AEE20 pKa = 4.35EE21 pKa = 4.08ILPKK25 pKa = 10.38FLTRR29 pKa = 11.84LKK31 pKa = 9.59TVAISTRR38 pKa = 11.84DD39 pKa = 3.53VVSVKK44 pKa = 9.7GTTDD48 pKa = 3.37LVDD51 pKa = 3.46IDD53 pKa = 4.7LLRR56 pKa = 11.84DD57 pKa = 3.49VPVNNWRR64 pKa = 11.84YY65 pKa = 8.87VGIVGIVASGEE76 pKa = 3.76WLLPDD81 pKa = 3.65NVSGGVAISFVDD93 pKa = 3.22KK94 pKa = 10.83RR95 pKa = 11.84LVDD98 pKa = 3.55SRR100 pKa = 11.84EE101 pKa = 4.45AILGTYY107 pKa = 9.34RR108 pKa = 11.84AAAVEE113 pKa = 4.04KK114 pKa = 10.27RR115 pKa = 11.84FQFKK119 pKa = 10.75LIPNYY124 pKa = 9.94FVSQEE129 pKa = 3.94DD130 pKa = 3.49ALRR133 pKa = 11.84RR134 pKa = 11.84PWQVQVSLKK143 pKa = 10.35GLKK146 pKa = 10.02FEE148 pKa = 5.12EE149 pKa = 5.17GFSPLTLEE157 pKa = 4.12FVSVVVCANSVVTKK171 pKa = 9.92GLRR174 pKa = 11.84EE175 pKa = 3.83RR176 pKa = 11.84LNNVGDD182 pKa = 4.04PNVEE186 pKa = 3.82VSEE189 pKa = 4.48VVVDD193 pKa = 3.89EE194 pKa = 4.7FVDD197 pKa = 4.48SISASQSLSRR207 pKa = 11.84ARR209 pKa = 11.84NKK211 pKa = 9.96YY212 pKa = 9.06VRR214 pKa = 11.84GNGKK218 pKa = 9.57VGNNSGGFSKK228 pKa = 10.94YY229 pKa = 9.77NRR231 pKa = 11.84HH232 pKa = 4.71QPEE235 pKa = 4.08RR236 pKa = 11.84FAGKK240 pKa = 10.53AMYY243 pKa = 9.98NSKK246 pKa = 10.61NVVRR250 pKa = 11.84GGTSEE255 pKa = 3.88PTAILHH261 pKa = 6.1KK262 pKa = 10.8RR263 pKa = 11.84MGGSNSIDD271 pKa = 3.06EE272 pKa = 4.67SLFSVAEE279 pKa = 4.05SDD281 pKa = 5.33LSDD284 pKa = 3.73SGCAA288 pKa = 3.53

Molecular weight: 31.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3133

134

1606

783.3

88.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.767 ± 0.467

1.564 ± 0.193

6.096 ± 0.306

6.192 ± 0.21

5.235 ± 0.201

4.915 ± 0.407

2.266 ± 0.414

4.692 ± 0.39

6.575 ± 0.48

9.863 ± 0.187

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.947 ± 0.136

4.852 ± 0.251

3.671 ± 0.199

3.288 ± 0.356

5.011 ± 0.532

7.533 ± 0.458

5.905 ± 0.703

9.129 ± 0.887

0.989 ± 0.071

3.479 ± 0.377
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski