Muricauda sp. ES.050
Average proteome isoelectric point is 6.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3184 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2N0GM34|A0A2N0GM34_9FLAO Uncharacterized protein OS=Muricauda sp. ES.050 OX=1798204 GN=AXZ78_3106 PE=4 SV=1
MM1 pKa = 7.66 KK2 pKa = 10.46 KK3 pKa = 10.01 NLPLKK8 pKa = 10.25 FSSFLFIFLSIMACSTEE25 pKa = 4.42 DD26 pKa = 3.28 GTNGLDD32 pKa = 4.09 GMDD35 pKa = 5.29 GIDD38 pKa = 4.9 GLDD41 pKa = 3.77 GSDD44 pKa = 4.72 GSDD47 pKa = 3.13 GLNSLVSTATEE58 pKa = 4.1 EE59 pKa = 4.63 PGGNCANGGFRR70 pKa = 11.84 LDD72 pKa = 4.5 FGLDD76 pKa = 3.19 SDD78 pKa = 4.78 NNGILDD84 pKa = 4.29 DD85 pKa = 4.5 NEE87 pKa = 4.3 VTASQYY93 pKa = 10.82 LCNVGPSEE101 pKa = 4.44 GLTSLISTVIEE112 pKa = 4.06 QPGDD116 pKa = 3.06 NCANGGYY123 pKa = 10.03 RR124 pKa = 11.84 LDD126 pKa = 3.75 VGLDD130 pKa = 3.39 NNSNGTLDD138 pKa = 3.49 VDD140 pKa = 3.92 EE141 pKa = 5.36 VSTSEE146 pKa = 4.2 YY147 pKa = 10.23 VCNGDD152 pKa = 4.19 SSDD155 pKa = 4.81 FSYY158 pKa = 11.11 QSYY161 pKa = 11.29 ASLISQTGTDD171 pKa = 4.39 DD172 pKa = 3.24 PTEE175 pKa = 4.0 QVLEE179 pKa = 4.03 NSLNLNISWARR190 pKa = 11.84 QSQGIYY196 pKa = 10.38 LGTLDD201 pKa = 3.68 TTIDD205 pKa = 3.07 ISKK208 pKa = 8.87 TVIFFSTPTTHH219 pKa = 5.93 TGVRR223 pKa = 11.84 GTLEE227 pKa = 3.81 SGNQIRR233 pKa = 11.84 LEE235 pKa = 4.02 LQNGINEE242 pKa = 4.16 FADD245 pKa = 3.38 NFEE248 pKa = 4.29 NLSFEE253 pKa = 4.0 LRR255 pKa = 11.84 EE256 pKa = 4.1 YY257 pKa = 10.48 EE258 pKa = 4.07
Molecular weight: 27.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.717
IPC2_protein 3.617
IPC_protein 3.63
Toseland 3.414
ProMoST 3.783
Dawson 3.63
Bjellqvist 3.808
Wikipedia 3.567
Rodwell 3.452
Grimsley 3.312
Solomon 3.617
Lehninger 3.567
Nozaki 3.745
DTASelect 3.973
Thurlkill 3.465
EMBOSS 3.567
Sillero 3.745
Patrickios 1.062
IPC_peptide 3.605
IPC2_peptide 3.732
IPC2.peptide.svr19 3.736
Protein with the highest isoelectric point:
>tr|A0A2N0GFJ4|A0A2N0GFJ4_9FLAO Uncharacterized protein DUF4270 OS=Muricauda sp. ES.050 OX=1798204 GN=AXZ78_0766 PE=4 SV=1
MM1 pKa = 7.84 PSGKK5 pKa = 9.32 KK6 pKa = 9.59 RR7 pKa = 11.84 KK8 pKa = 7.05 RR9 pKa = 11.84 HH10 pKa = 5.11 KK11 pKa = 10.59 VATHH15 pKa = 5.21 KK16 pKa = 10.3 RR17 pKa = 11.84 KK18 pKa = 9.62 KK19 pKa = 9.04 RR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 ANRR25 pKa = 11.84 HH26 pKa = 4.5 KK27 pKa = 10.78 KK28 pKa = 9.37 KK29 pKa = 10.83
Molecular weight: 3.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.474
IPC2_protein 11.082
IPC_protein 12.618
Toseland 12.808
ProMoST 13.276
Dawson 12.808
Bjellqvist 12.793
Wikipedia 13.261
Rodwell 12.705
Grimsley 12.837
Solomon 13.29
Lehninger 13.188
Nozaki 12.793
DTASelect 12.793
Thurlkill 12.793
EMBOSS 13.29
Sillero 12.793
Patrickios 12.427
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.035
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3184
0
3184
1083582
29
4729
340.3
38.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.496 ± 0.049
0.718 ± 0.015
5.89 ± 0.05
6.985 ± 0.042
5.072 ± 0.037
6.895 ± 0.049
1.835 ± 0.022
7.264 ± 0.04
7.339 ± 0.073
9.29 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.456 ± 0.023
5.614 ± 0.04
3.62 ± 0.025
3.439 ± 0.024
3.608 ± 0.032
6.309 ± 0.03
5.63 ± 0.065
6.487 ± 0.033
1.109 ± 0.018
3.944 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here