Streptococcus satellite phage Javan756

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 17 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A176|A0A4D6A176_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan756 OX=2558851 GN=JavanS756_0016 PE=4 SV=1
MM1 pKa = 7.03LTEE4 pKa = 4.97LFNLSPLFVTLYY16 pKa = 10.81VFSAYY21 pKa = 10.75LNLIVCEE28 pKa = 4.16EE29 pKa = 4.04IKK31 pKa = 10.89LDD33 pKa = 4.49LEE35 pKa = 4.42QAA37 pKa = 3.63

Molecular weight: 4.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZW01|A0A4D5ZW01_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan756 OX=2558851 GN=JavanS756_0013 PE=4 SV=1
MM1 pKa = 7.84KK2 pKa = 9.12ITEE5 pKa = 4.39VIKK8 pKa = 10.77KK9 pKa = 10.02DD10 pKa = 3.19GSKK13 pKa = 10.5VYY15 pKa = 10.16RR16 pKa = 11.84ANIYY20 pKa = 10.65LGVDD24 pKa = 2.99QVTGKK29 pKa = 10.2KK30 pKa = 10.32VKK32 pKa = 9.81TKK34 pKa = 9.67VTGRR38 pKa = 11.84TQKK41 pKa = 10.03EE42 pKa = 4.46VKK44 pKa = 10.09QKK46 pKa = 9.49ATQEE50 pKa = 4.02KK51 pKa = 10.11IAFQKK56 pKa = 10.81AGSTRR61 pKa = 11.84QKK63 pKa = 11.11ASTIKK68 pKa = 10.57NYY70 pKa = 10.28QEE72 pKa = 4.69LANLWLEE79 pKa = 4.39SYY81 pKa = 11.14KK82 pKa = 10.15NTVKK86 pKa = 10.74PNTQDD91 pKa = 3.13NVRR94 pKa = 11.84KK95 pKa = 10.11LIDD98 pKa = 3.43NHH100 pKa = 6.27ILPIFGAYY108 pKa = 9.61KK109 pKa = 10.19LDD111 pKa = 3.67KK112 pKa = 10.12LTTPLIQSIINEE124 pKa = 4.36LADD127 pKa = 3.28KK128 pKa = 10.13TNRR131 pKa = 11.84GEE133 pKa = 4.1KK134 pKa = 10.44GAFLHH139 pKa = 6.19YY140 pKa = 10.82DD141 pKa = 4.02KK142 pKa = 11.05IHH144 pKa = 6.59ALNKK148 pKa = 10.18RR149 pKa = 11.84ILQYY153 pKa = 10.89GVTMQAIPSNPARR166 pKa = 11.84DD167 pKa = 3.48VVLPRR172 pKa = 11.84NTQKK176 pKa = 11.2AKK178 pKa = 9.91RR179 pKa = 11.84KK180 pKa = 8.83KK181 pKa = 9.6VKK183 pKa = 10.32HH184 pKa = 5.92FEE186 pKa = 3.96NQEE189 pKa = 3.98LKK191 pKa = 10.93KK192 pKa = 10.66FLAYY196 pKa = 10.52LDD198 pKa = 4.54NLDD201 pKa = 3.52SDD203 pKa = 4.32RR204 pKa = 11.84YY205 pKa = 8.96RR206 pKa = 11.84YY207 pKa = 10.17YY208 pKa = 11.31YY209 pKa = 8.28EE210 pKa = 3.62TTLYY214 pKa = 10.88KK215 pKa = 10.46FLLATGCRR223 pKa = 11.84INEE226 pKa = 4.0ALALSWSDD234 pKa = 2.88IDD236 pKa = 4.45LDD238 pKa = 3.82NAVVHH243 pKa = 5.2VTKK246 pKa = 10.18TLNRR250 pKa = 11.84DD251 pKa = 3.49LEE253 pKa = 4.53INSPKK258 pKa = 10.62SKK260 pKa = 10.68ASYY263 pKa = 10.37RR264 pKa = 11.84DD265 pKa = 2.95IDD267 pKa = 3.41IDD269 pKa = 3.65QATVSMLKK277 pKa = 9.91QYY279 pKa = 11.27KK280 pKa = 9.69LRR282 pKa = 11.84QTKK285 pKa = 7.97EE286 pKa = 3.19AWKK289 pKa = 9.48IGQRR293 pKa = 11.84EE294 pKa = 4.21SVVFSDD300 pKa = 6.46FIHH303 pKa = 7.06EE304 pKa = 4.42YY305 pKa = 9.91PSSSRR310 pKa = 11.84LKK312 pKa = 10.58RR313 pKa = 11.84RR314 pKa = 11.84LQTHH318 pKa = 6.76FKK320 pKa = 10.46RR321 pKa = 11.84ADD323 pKa = 3.23VPNIGFHH330 pKa = 6.1GFRR333 pKa = 11.84HH334 pKa = 4.87THH336 pKa = 6.58ASLLLNSGIPYY347 pKa = 10.03KK348 pKa = 10.3EE349 pKa = 3.58LQYY352 pKa = 11.41RR353 pKa = 11.84LGHH356 pKa = 5.67STLSMTMDD364 pKa = 3.58IYY366 pKa = 11.68SHH368 pKa = 6.98LSKK371 pKa = 11.0EE372 pKa = 4.25NAKK375 pKa = 10.37KK376 pKa = 10.37AVSFYY381 pKa = 9.42EE382 pKa = 4.24TALKK386 pKa = 10.67ALL388 pKa = 4.15

Molecular weight: 44.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

17

0

17

2696

34

388

158.6

18.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.341 ± 0.417

0.668 ± 0.162

5.749 ± 0.516

9.088 ± 0.663

3.932 ± 0.271

4.154 ± 0.35

1.966 ± 0.336

7.493 ± 0.703

9.792 ± 0.461

10.312 ± 0.593

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.634 ± 0.274

5.378 ± 0.369

2.596 ± 0.321

4.228 ± 0.435

4.97 ± 0.38

5.861 ± 0.289

6.269 ± 0.532

4.636 ± 0.386

0.556 ± 0.104

4.377 ± 0.339
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski