Egaro virus
Average proteome isoelectric point is 8.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B2RVN9|A0A1B2RVN9_9VIRU RNA-dependent RNA polymerase OS=Egaro virus OX=1888318 PE=4 SV=1
MM1 pKa = 7.32 MNMRR5 pKa = 11.84 LNPSSEE11 pKa = 4.02 PLDD14 pKa = 3.81 VQQLDD19 pKa = 3.95 FLTEE23 pKa = 4.04 LSPFPVFNLQNAQKK37 pKa = 9.01 MQIHH41 pKa = 6.37 PPKK44 pKa = 10.86 VNMTGGFVSVARR56 pKa = 11.84 SFGKK60 pKa = 9.87 EE61 pKa = 3.29 AARR64 pKa = 11.84 DD65 pKa = 3.53 RR66 pKa = 11.84 RR67 pKa = 11.84 LLGGALAYY75 pKa = 7.6 FTQNEE80 pKa = 4.23 VAEE83 pKa = 4.37 ILQKK87 pKa = 9.64 TVFCYY92 pKa = 9.35 GTAKK96 pKa = 10.67 GFLTRR101 pKa = 11.84 LKK103 pKa = 10.59 EE104 pKa = 3.92 LTTRR108 pKa = 11.84 DD109 pKa = 3.62 CKK111 pKa = 10.48 TPYY114 pKa = 10.25 SISQTVSILNKK125 pKa = 9.95 RR126 pKa = 11.84 MPVLAEE132 pKa = 4.4 EE133 pKa = 4.46 LPSWEE138 pKa = 4.9 DD139 pKa = 3.38 FLSLLEE145 pKa = 4.37 NTDD148 pKa = 3.38 LTYY151 pKa = 10.67 SASAGPPYY159 pKa = 9.71 WRR161 pKa = 11.84 SKK163 pKa = 10.95 LMSLEE168 pKa = 4.09 DD169 pKa = 3.68 MEE171 pKa = 6.18 LEE173 pKa = 4.13 VLPLVLEE180 pKa = 4.17 HH181 pKa = 6.36 MKK183 pKa = 10.82 AGTLNQLYY191 pKa = 9.64 HH192 pKa = 6.29 LHH194 pKa = 7.14 PEE196 pKa = 4.29 LFLVEE201 pKa = 4.67 IKK203 pKa = 10.98 NKK205 pKa = 9.72 LDD207 pKa = 3.42 RR208 pKa = 11.84 YY209 pKa = 7.93 EE210 pKa = 3.95 TAKK213 pKa = 10.61 FDD215 pKa = 4.16 VKK217 pKa = 10.47 CRR219 pKa = 11.84 PYY221 pKa = 11.27 GNVPFHH227 pKa = 6.41 MGFLASMIVQPFCKK241 pKa = 10.01 KK242 pKa = 10.4 LKK244 pKa = 9.58 LWHH247 pKa = 6.9 NSDD250 pKa = 2.71 SWNAYY255 pKa = 8.48 GFSFAEE261 pKa = 4.41 GSYY264 pKa = 10.95 LEE266 pKa = 4.52 LYY268 pKa = 9.91 EE269 pKa = 4.2 RR270 pKa = 11.84 LKK272 pKa = 10.7 KK273 pKa = 10.58 FKK275 pKa = 10.71 RR276 pKa = 11.84 GDD278 pKa = 3.45 EE279 pKa = 4.18 PLVGVYY285 pKa = 10.75 GDD287 pKa = 3.72 DD288 pKa = 3.68 VEE290 pKa = 5.05 IYY292 pKa = 10.15 FYY294 pKa = 11.6 ANKK297 pKa = 10.09 KK298 pKa = 10.07 LYY300 pKa = 10.79 AVDD303 pKa = 4.13 PDD305 pKa = 4.4 FKK307 pKa = 11.52 QMDD310 pKa = 3.95 GSVDD314 pKa = 3.94 LVTVKK319 pKa = 10.57 AVCGYY324 pKa = 10.3 INYY327 pKa = 9.82 AFEE330 pKa = 4.57 RR331 pKa = 11.84 KK332 pKa = 8.87 WGEE335 pKa = 3.64 NQLWRR340 pKa = 11.84 EE341 pKa = 3.97 VLKK344 pKa = 11.0 LLSKK348 pKa = 10.73 LATSPPMIIHH358 pKa = 6.53 GSKK361 pKa = 10.2 VFRR364 pKa = 11.84 KK365 pKa = 9.28 KK366 pKa = 10.67 SSGGIISGVVGTTLFDD382 pKa = 3.48 TAKK385 pKa = 10.71 SIVAYY390 pKa = 10.02 HH391 pKa = 6.6 HH392 pKa = 6.59 LLEE395 pKa = 5.07 HH396 pKa = 5.75 VQHH399 pKa = 7.55 DD400 pKa = 4.3 YY401 pKa = 11.06 TLLLDD406 pKa = 5.24 AGFCKK411 pKa = 10.25 QFLKK415 pKa = 10.15 QHH417 pKa = 6.4 HH418 pKa = 6.26 ALEE421 pKa = 4.63 VKK423 pKa = 9.95 QDD425 pKa = 2.79 SWNPVEE431 pKa = 5.43 VNMNPVHH438 pKa = 7.01 LEE440 pKa = 3.69 PFVEE444 pKa = 4.4 NKK446 pKa = 9.99 FLGVMRR452 pKa = 11.84 YY453 pKa = 7.89 WYY455 pKa = 9.85 EE456 pKa = 4.03 LDD458 pKa = 4.1 DD459 pKa = 3.73 STFIIVPYY467 pKa = 9.99 LHH469 pKa = 6.37 EE470 pKa = 5.5 HH471 pKa = 6.88 DD472 pKa = 3.8 WLTNILAPRR481 pKa = 11.84 TVDD484 pKa = 3.64 SISGPTRR491 pKa = 11.84 KK492 pKa = 9.61 PSDD495 pKa = 3.42 MTSEE499 pKa = 4.65 RR500 pKa = 11.84 ISFDD504 pKa = 3.07 RR505 pKa = 11.84 ARR507 pKa = 11.84 GLLVTGGIFNKK518 pKa = 10.11 KK519 pKa = 9.56 IKK521 pKa = 10.24 DD522 pKa = 3.71 ALYY525 pKa = 10.44 CAIDD529 pKa = 4.82 KK530 pKa = 10.73 IPSLPIVMHH539 pKa = 5.43 VQAGGGRR546 pKa = 11.84 GEE548 pKa = 4.84 KK549 pKa = 10.04 PGEE552 pKa = 4.14 TNVVGNNFSFTDD564 pKa = 3.13 SHH566 pKa = 6.77 MIPSVGFVEE575 pKa = 6.06 DD576 pKa = 3.78 IYY578 pKa = 11.33 TPIHH582 pKa = 6.26 RR583 pKa = 11.84 RR584 pKa = 11.84 KK585 pKa = 9.53 GHH587 pKa = 6.13 PPVYY591 pKa = 10.13 LYY593 pKa = 7.6 PTLVEE598 pKa = 3.94 QLNLSRR604 pKa = 11.84 NKK606 pKa = 10.03 NRR608 pKa = 11.84 LVEE611 pKa = 3.99 AFKK614 pKa = 10.9 CFKK617 pKa = 10.89 VVDD620 pKa = 3.91 NTTTRR625 pKa = 11.84 HH626 pKa = 5.09 VKK628 pKa = 8.93 GQYY631 pKa = 9.12 VKK633 pKa = 10.17 PVEE636 pKa = 4.28 RR637 pKa = 11.84 NLEE640 pKa = 4.35 SKK642 pKa = 10.88 VPEE645 pKa = 3.9 HH646 pKa = 7.28 VNFSKK651 pKa = 11.12 LNLPFAFGKK660 pKa = 9.39 TSGAKK665 pKa = 9.64 PLQQLSDD672 pKa = 3.75 YY673 pKa = 9.83 AYY675 pKa = 8.76 HH676 pKa = 6.13 HH677 pKa = 5.81 TVEE680 pKa = 5.12 FGPMPKK686 pKa = 10.02 SFVSQFVPIPQNLAPKK702 pKa = 10.5 APILQAYY709 pKa = 8.46 AKK711 pKa = 10.39 LNLVPDD717 pKa = 4.34 GWNEE721 pKa = 3.75 TNIRR725 pKa = 11.84 KK726 pKa = 9.7 SKK728 pKa = 10.68 LSSLYY733 pKa = 11.04 NKK735 pKa = 10.42 DD736 pKa = 2.55 ITYY739 pKa = 10.1 KK740 pKa = 10.58 HH741 pKa = 6.08 AVEE744 pKa = 5.33 KK745 pKa = 10.16 IDD747 pKa = 3.97 RR748 pKa = 11.84 LIKK751 pKa = 10.86 ARR753 pKa = 11.84 DD754 pKa = 3.53 TPPAVDD760 pKa = 3.58 VTPQLLIEE768 pKa = 4.45 GVVDD772 pKa = 2.9 IHH774 pKa = 7.67 VEE776 pKa = 3.92 NVKK779 pKa = 10.37 RR780 pKa = 11.84 AFVVYY785 pKa = 9.64 PLYY788 pKa = 10.85 EE789 pKa = 4.19 DD790 pKa = 4.36 TGLHH794 pKa = 5.45 WLSSIFQQNSLKK806 pKa = 10.55 KK807 pKa = 8.68 RR808 pKa = 11.84 WSTEE812 pKa = 3.4 VTEE815 pKa = 4.44 SKK817 pKa = 10.42 KK818 pKa = 10.38 VQLSAEE824 pKa = 4.24 SLQGAVIPLARR835 pKa = 11.84 VTGKK839 pKa = 10.34 DD840 pKa = 3.02 LAASLKK846 pKa = 10.8 LKK848 pKa = 10.19 IAQEE852 pKa = 3.88 LFNRR856 pKa = 11.84 LRR858 pKa = 11.84 AEE860 pKa = 4.38 PDD862 pKa = 3.27 ITWTPVGKK870 pKa = 9.83 KK871 pKa = 9.51 GKK873 pKa = 10.28 RR874 pKa = 11.84 IGEE877 pKa = 4.39 SVANTSDD884 pKa = 3.27 NVNVAEE890 pKa = 5.03 GPIKK894 pKa = 10.51 DD895 pKa = 3.78 WSNMIEE901 pKa = 4.39 TINPSDD907 pKa = 3.64 MVVEE911 pKa = 4.27 DD912 pKa = 4.6 FKK914 pKa = 11.65 KK915 pKa = 10.81 LEE917 pKa = 4.22 YY918 pKa = 10.61 YY919 pKa = 11.16 SNLLKK924 pKa = 10.21 TKK926 pKa = 10.15 LKK928 pKa = 10.14 QFEE931 pKa = 4.59 EE932 pKa = 4.26 INKK935 pKa = 9.74 KK936 pKa = 10.5 DD937 pKa = 3.46 EE938 pKa = 4.48 NKK940 pKa = 10.74 NKK942 pKa = 10.04 TEE944 pKa = 3.82 FDD946 pKa = 3.05 QTRR949 pKa = 11.84 RR950 pKa = 11.84 NFGGVNEE957 pKa = 5.09 SSNQSKK963 pKa = 10.24 NRR965 pKa = 11.84 LSGARR970 pKa = 11.84 KK971 pKa = 8.26 KK972 pKa = 8.91 TSKK975 pKa = 10.42 KK976 pKa = 9.31 KK977 pKa = 7.7 EE978 pKa = 3.75 QRR980 pKa = 11.84 EE981 pKa = 4.01 VSTGGNANASTEE993 pKa = 4.39 FRR995 pKa = 11.84 FNWII999 pKa = 3.36
Molecular weight: 113.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.427
IPC2_protein 8.375
IPC_protein 8.229
Toseland 8.96
ProMoST 8.799
Dawson 9.282
Bjellqvist 9.063
Wikipedia 9.428
Rodwell 9.531
Grimsley 9.326
Solomon 9.311
Lehninger 9.268
Nozaki 9.165
DTASelect 8.99
Thurlkill 9.151
EMBOSS 9.428
Sillero 9.282
Patrickios 4.724
IPC_peptide 9.311
IPC2_peptide 7.6
IPC2.peptide.svr19 7.628
Protein with the highest isoelectric point:
>tr|A0A1B2RVN9|A0A1B2RVN9_9VIRU RNA-dependent RNA polymerase OS=Egaro virus OX=1888318 PE=4 SV=1
MM1 pKa = 6.92 KK2 pKa = 9.99 TKK4 pKa = 10.06 TKK6 pKa = 10.68 QNLIKK11 pKa = 10.22 QDD13 pKa = 3.46 GTLEE17 pKa = 4.11 EE18 pKa = 4.35 LMKK21 pKa = 10.93 AQIKK25 pKa = 10.43 AKK27 pKa = 9.5 IASLAPVKK35 pKa = 10.23 RR36 pKa = 11.84 RR37 pKa = 11.84 RR38 pKa = 11.84 KK39 pKa = 9.39 RR40 pKa = 11.84 RR41 pKa = 11.84 NKK43 pKa = 10.34 GKK45 pKa = 9.85 FPPVAMPMPQRR56 pKa = 11.84 SFALTGFNKK65 pKa = 10.55 DD66 pKa = 3.23 EE67 pKa = 4.26 GVDD70 pKa = 3.97 RR71 pKa = 11.84 IFHH74 pKa = 6.45 IPDD77 pKa = 2.93 ISKK80 pKa = 10.79 DD81 pKa = 3.46 IVDD84 pKa = 3.88 KK85 pKa = 11.19 QILIDD90 pKa = 3.97 IPVLPTLGSRR100 pKa = 11.84 LGIIASAWQRR110 pKa = 11.84 IVYY113 pKa = 9.75 KK114 pKa = 10.35 KK115 pKa = 10.8 LEE117 pKa = 3.98 FSITPKK123 pKa = 10.23 TSTAVSGGYY132 pKa = 9.01 IAAFIPDD139 pKa = 3.73 VTDD142 pKa = 3.27 VLTGTSPDD150 pKa = 3.41 FALDD154 pKa = 3.64 KK155 pKa = 11.07 LSSQPSAVEE164 pKa = 3.98 AKK166 pKa = 7.96 WWEE169 pKa = 4.14 SSTVRR174 pKa = 11.84 VPSLPDD180 pKa = 3.32 LFYY183 pKa = 10.52 TSPSPEE189 pKa = 3.98 SPRR192 pKa = 11.84 FSSPGRR198 pKa = 11.84 FVLIVDD204 pKa = 4.71 GAASQAGSLTIKK216 pKa = 8.08 MHH218 pKa = 5.77 WSVLLSEE225 pKa = 5.17 PSLEE229 pKa = 4.37 GNNAPADD236 pKa = 3.52 QKK238 pKa = 11.46 EE239 pKa = 4.08 FVIKK243 pKa = 10.7 DD244 pKa = 4.02 GLWMQAKK251 pKa = 10.32 AGGIFVNTSSDD262 pKa = 3.34 NTKK265 pKa = 10.54 KK266 pKa = 10.74 VLNSNVLLVLPDD278 pKa = 3.41 AKK280 pKa = 10.62 EE281 pKa = 3.8 NQIYY285 pKa = 10.65 RR286 pKa = 11.84 MATTASYY293 pKa = 10.37 LRR295 pKa = 11.84 CADD298 pKa = 3.63 TVTLDD303 pKa = 4.03 NIWLRR308 pKa = 11.84 SFWYY312 pKa = 9.39 FIIVKK317 pKa = 8.8 TSSGGLQMYY326 pKa = 8.69 PYY328 pKa = 9.57 TPEE331 pKa = 3.7 NKK333 pKa = 9.95 EE334 pKa = 3.78 ILKK337 pKa = 10.79 DD338 pKa = 3.61 NSAGDD343 pKa = 4.07 TEE345 pKa = 4.61 VLPAGHH351 pKa = 6.48 EE352 pKa = 4.2 VLLEE356 pKa = 3.86 STAQVDD362 pKa = 4.48 FPRR365 pKa = 11.84 GSEE368 pKa = 4.31 FICASDD374 pKa = 3.47 RR375 pKa = 11.84 KK376 pKa = 10.38 SLLHH380 pKa = 6.57 HH381 pKa = 7.11 FYY383 pKa = 11.25 QMSLSLNKK391 pKa = 9.65 NRR393 pKa = 11.84 QQYY396 pKa = 7.31 QTASSNKK403 pKa = 9.34 CLTFLPLYY411 pKa = 10.55 SKK413 pKa = 10.57 FISSLKK419 pKa = 10.37 RR420 pKa = 11.84 EE421 pKa = 4.08 DD422 pKa = 3.52 SFGEE426 pKa = 4.05 EE427 pKa = 3.57 KK428 pKa = 10.66 ALRR431 pKa = 11.84 WRR433 pKa = 11.84 AGSQASFLDD442 pKa = 3.95 WEE444 pKa = 4.49 TLPEE448 pKa = 4.15 KK449 pKa = 9.98 LTRR452 pKa = 11.84 LEE454 pKa = 4.14 VSDD457 pKa = 4.0 VQSLKK462 pKa = 11.09
Molecular weight: 51.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.689
IPC2_protein 8.536
IPC_protein 8.434
Toseland 9.311
ProMoST 9.063
Dawson 9.545
Bjellqvist 9.238
Wikipedia 9.677
Rodwell 9.926
Grimsley 9.604
Solomon 9.589
Lehninger 9.56
Nozaki 9.399
DTASelect 9.194
Thurlkill 9.399
EMBOSS 9.736
Sillero 9.502
Patrickios 5.258
IPC_peptide 9.589
IPC2_peptide 7.702
IPC2.peptide.svr19 7.779
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1461
462
999
730.5
82.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.955 ± 0.658
0.753 ± 0.057
5.065 ± 0.312
6.023 ± 0.579
4.791 ± 0.136
5.407 ± 0.238
2.19 ± 0.614
4.86 ± 0.545
8.487 ± 0.265
10.062 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.053 ± 0.058
4.928 ± 0.811
5.613 ± 0.128
3.628 ± 0.269
4.517 ± 0.135
8.008 ± 1.439
5.544 ± 0.286
7.255 ± 0.781
1.643 ± 0.169
3.217 ± 0.463
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here