Selenomonas sp. mPRGC5

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Selenomonadales; Selenomonadaceae; Selenomonas; unclassified Selenomonas

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2656 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5D6VYR5|A0A5D6VYR5_9FIRM DASS family sodium-coupled anion symporter OS=Selenomonas sp. mPRGC5 OX=2593411 GN=FZ040_11205 PE=3 SV=1
MM1 pKa = 7.36TVTTQGKK8 pKa = 8.87EE9 pKa = 3.64LACIDD14 pKa = 4.63KK15 pKa = 10.66LGTMCAMSHH24 pKa = 4.95WTDD27 pKa = 3.66DD28 pKa = 3.54QLQDD32 pKa = 3.67VLDD35 pKa = 3.75MLRR38 pKa = 11.84DD39 pKa = 3.72YY40 pKa = 11.01GQSCEE45 pKa = 4.05QDD47 pKa = 3.14GFEE50 pKa = 4.77AGYY53 pKa = 9.85EE54 pKa = 3.99AALNDD59 pKa = 3.48AVAAAEE65 pKa = 4.08RR66 pKa = 11.84TAMGG70 pKa = 3.76

Molecular weight: 7.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5D6W6I7|A0A5D6W6I7_9FIRM Beta-1 4-N-acetylgalactosaminyltransferase OS=Selenomonas sp. mPRGC5 OX=2593411 GN=FZ040_04965 PE=4 SV=1
MM1 pKa = 6.8QQLLGYY7 pKa = 9.53VRR9 pKa = 11.84VSTRR13 pKa = 11.84EE14 pKa = 3.81QNLDD18 pKa = 3.32RR19 pKa = 11.84QLAAMKK25 pKa = 10.62RR26 pKa = 11.84MGIKK30 pKa = 9.72PKK32 pKa = 10.37NIYY35 pKa = 8.62MDD37 pKa = 3.81KK38 pKa = 11.08LSGKK42 pKa = 10.01DD43 pKa = 3.59FSRR46 pKa = 11.84PQYY49 pKa = 9.29QKK51 pKa = 11.06LMRR54 pKa = 11.84RR55 pKa = 11.84INSEE59 pKa = 4.19TILCIHH65 pKa = 6.93SIDD68 pKa = 3.97RR69 pKa = 11.84LGRR72 pKa = 11.84NYY74 pKa = 10.81KK75 pKa = 10.03EE76 pKa = 4.86VLEE79 pKa = 4.13QWRR82 pKa = 11.84QITKK86 pKa = 10.21IKK88 pKa = 10.44GADD91 pKa = 3.05IVVLDD96 pKa = 4.51MPLLDD101 pKa = 3.66TRR103 pKa = 11.84KK104 pKa = 10.26GRR106 pKa = 11.84DD107 pKa = 3.3LLGTFVSDD115 pKa = 4.7VILQVLSFVAEE126 pKa = 3.9NEE128 pKa = 3.6RR129 pKa = 11.84RR130 pKa = 11.84NIRR133 pKa = 11.84KK134 pKa = 8.83RR135 pKa = 11.84QAEE138 pKa = 4.73GIAEE142 pKa = 3.88ARR144 pKa = 11.84KK145 pKa = 9.21RR146 pKa = 11.84GVRR149 pKa = 11.84FGRR152 pKa = 11.84PCKK155 pKa = 9.57PLPPGFLQAYY165 pKa = 6.57AAWRR169 pKa = 11.84EE170 pKa = 3.88GRR172 pKa = 11.84MSMNQAARR180 pKa = 11.84QINMPVSTFYY190 pKa = 11.23NKK192 pKa = 9.9CQQRR196 pKa = 11.84KK197 pKa = 8.52KK198 pKa = 10.62RR199 pKa = 11.84EE200 pKa = 3.76RR201 pKa = 3.41

Molecular weight: 23.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2656

0

2656

875554

25

6826

329.7

36.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.355 ± 0.057

1.18 ± 0.027

5.665 ± 0.046

6.894 ± 0.062

3.871 ± 0.051

7.483 ± 0.066

2.006 ± 0.022

6.498 ± 0.042

6.22 ± 0.045

9.221 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.139 ± 0.035

3.946 ± 0.062

3.637 ± 0.042

3.769 ± 0.034

4.879 ± 0.052

5.391 ± 0.047

5.276 ± 0.056

7.16 ± 0.041

0.984 ± 0.018

3.428 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski