Crassaminicella sp. SY095

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Crassaminicella; unclassified Crassaminicella

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2867 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C0SH31|A0A5C0SH31_9CLOT DUF3189 family protein OS=Crassaminicella sp. SY095 OX=2599308 GN=FQB35_08465 PE=4 SV=1
MM1 pKa = 7.45NYY3 pKa = 10.07LYY5 pKa = 10.85EE6 pKa = 4.33RR7 pKa = 11.84VSYY10 pKa = 10.79LRR12 pKa = 11.84GLADD16 pKa = 3.47GMEE19 pKa = 4.21LSEE22 pKa = 4.17EE23 pKa = 4.21SKK25 pKa = 10.76EE26 pKa = 4.52GKK28 pKa = 10.14LLLNMLDD35 pKa = 3.69VLEE38 pKa = 5.26DD39 pKa = 3.41FADD42 pKa = 5.11AINEE46 pKa = 4.0VHH48 pKa = 7.63DD49 pKa = 4.5DD50 pKa = 3.92VKK52 pKa = 11.48DD53 pKa = 3.63LDD55 pKa = 5.15DD56 pKa = 3.91YY57 pKa = 12.16VEE59 pKa = 4.88TIDD62 pKa = 4.0EE63 pKa = 4.32DD64 pKa = 3.9LAAVEE69 pKa = 4.95DD70 pKa = 4.4EE71 pKa = 4.32VFEE74 pKa = 4.99EE75 pKa = 4.5IDD77 pKa = 3.36TEE79 pKa = 4.3EE80 pKa = 4.24FNEE83 pKa = 4.34DD84 pKa = 3.94DD85 pKa = 3.6IDD87 pKa = 4.66FIEE90 pKa = 4.94VKK92 pKa = 10.62CPNCKK97 pKa = 10.12EE98 pKa = 4.0IIYY101 pKa = 10.4LDD103 pKa = 3.81EE104 pKa = 4.47NLLNNQDD111 pKa = 4.41DD112 pKa = 4.42EE113 pKa = 5.04TEE115 pKa = 4.45VICPSCHH122 pKa = 4.74EE123 pKa = 4.56KK124 pKa = 10.3IYY126 pKa = 10.59IEE128 pKa = 4.4EE129 pKa = 4.36NCDD132 pKa = 5.06HH133 pKa = 7.62DD134 pKa = 4.55NCCCNDD140 pKa = 3.29HH141 pKa = 6.97NEE143 pKa = 3.93

Molecular weight: 16.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C0SG96|A0A5C0SG96_9CLOT Glycine dehydrogenase (aminomethyl-transferring) OS=Crassaminicella sp. SY095 OX=2599308 GN=FQB35_05345 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.67QPKK8 pKa = 9.0KK9 pKa = 7.87RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.39KK14 pKa = 8.49EE15 pKa = 3.5HH16 pKa = 6.09GFRR19 pKa = 11.84KK20 pKa = 9.96RR21 pKa = 11.84MSSKK25 pKa = 10.25SGRR28 pKa = 11.84NVLKK32 pKa = 10.5RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.18GRR39 pKa = 11.84KK40 pKa = 8.99RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2867

0

2867

861284

20

2846

300.4

33.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.02 ± 0.054

1.056 ± 0.019

5.311 ± 0.032

7.586 ± 0.046

4.263 ± 0.032

6.611 ± 0.045

1.571 ± 0.016

10.299 ± 0.05

9.528 ± 0.055

8.941 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.799 ± 0.024

5.452 ± 0.038

3.042 ± 0.029

2.62 ± 0.025

3.612 ± 0.03

5.337 ± 0.032

4.752 ± 0.035

6.605 ± 0.041

0.684 ± 0.016

3.912 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski