Mesoplasma florum (strain ATCC 33453 / NBRC 100688 / NCTC 11704 / L1) (Acholeplasma florum)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Entomoplasmatales; Entomoplasmataceae; Mesoplasma; Mesoplasma florum

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 683 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q6F101|YQGF_MESFL Putative pre-16S rRNA nuclease OS=Mesoplasma florum (strain ATCC 33453 / NBRC 100688 / NCTC 11704 / L1) OX=265311 GN=Mfl464 PE=3 SV=1
MM1 pKa = 7.56TKK3 pKa = 10.39FKK5 pKa = 11.47YY6 pKa = 10.11EE7 pKa = 3.8DD8 pKa = 2.99WLMQFINDD16 pKa = 3.53DD17 pKa = 3.38WYY19 pKa = 10.2IQINTSEE26 pKa = 3.98NNIIFDD32 pKa = 3.95EE33 pKa = 4.73VIQLHH38 pKa = 5.83EE39 pKa = 4.64KK40 pKa = 9.56WLDD43 pKa = 3.45SQDD46 pKa = 3.39YY47 pKa = 11.37NNFIKK52 pKa = 10.49EE53 pKa = 4.0NQEE56 pKa = 3.42AVAIDD61 pKa = 3.93NLPGFLEE68 pKa = 4.14NEE70 pKa = 4.52EE71 pKa = 4.16VCKK74 pKa = 9.66TDD76 pKa = 3.64EE77 pKa = 4.44YY78 pKa = 10.75IKK80 pKa = 10.98SFISGVFHH88 pKa = 7.13LRR90 pKa = 11.84IDD92 pKa = 3.58GLYY95 pKa = 10.43NIASDD100 pKa = 3.82YY101 pKa = 11.52VNAFNEE107 pKa = 4.29INEE110 pKa = 4.32HH111 pKa = 5.65SFNAVDD117 pKa = 3.59EE118 pKa = 4.81SGVDD122 pKa = 3.49VAINKK127 pKa = 9.36AFLEE131 pKa = 4.21LSEE134 pKa = 4.79KK135 pKa = 10.79YY136 pKa = 9.73YY137 pKa = 11.07EE138 pKa = 4.02EE139 pKa = 5.85LITVVRR145 pKa = 11.84NTEE148 pKa = 3.8VPDD151 pKa = 3.31EE152 pKa = 5.03FKK154 pKa = 11.26YY155 pKa = 10.58CWRR158 pKa = 11.84DD159 pKa = 3.36LIEE162 pKa = 4.13LVQRR166 pKa = 11.84FNSYY170 pKa = 8.54EE171 pKa = 4.02SRR173 pKa = 11.84EE174 pKa = 4.24DD175 pKa = 3.63KK176 pKa = 11.24LDD178 pKa = 3.39VAYY181 pKa = 10.77QLLDD185 pKa = 3.61YY186 pKa = 9.49LTTTIDD192 pKa = 3.55GFDD195 pKa = 4.48DD196 pKa = 4.72LSIDD200 pKa = 3.84LTDD203 pKa = 4.22EE204 pKa = 4.52MIEE207 pKa = 4.04SSNNFIALLIKK218 pKa = 10.1FEE220 pKa = 4.93IIFDD224 pKa = 3.85RR225 pKa = 11.84LILLKK230 pKa = 10.29EE231 pKa = 4.36HH232 pKa = 7.18IEE234 pKa = 4.04YY235 pKa = 10.59QYY237 pKa = 11.46VEE239 pKa = 4.28QKK241 pKa = 10.82GLPDD245 pKa = 3.06NFYY248 pKa = 11.31RR249 pKa = 11.84MNILDD254 pKa = 3.93RR255 pKa = 11.84YY256 pKa = 10.58KK257 pKa = 10.55EE258 pKa = 3.89IEE260 pKa = 4.04TFKK263 pKa = 10.99IMNEE267 pKa = 3.54EE268 pKa = 4.09DD269 pKa = 3.05

Molecular weight: 32.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6F0D7|Q6F0D7_MESFL Cof-like hydrolase HAD-superfamily OS=Mesoplasma florum (strain ATCC 33453 / NBRC 100688 / NCTC 11704 / L1) OX=265311 GN=Mfl680 PE=4 SV=1
MM1 pKa = 7.32KK2 pKa = 9.47RR3 pKa = 11.84TWQPSKK9 pKa = 10.02IKK11 pKa = 9.82HH12 pKa = 5.45ARR14 pKa = 11.84VHH16 pKa = 5.94GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.41NGRR28 pKa = 11.84KK29 pKa = 9.2VIKK32 pKa = 10.14ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.33GRR39 pKa = 11.84AKK41 pKa = 9.64LTAA44 pKa = 4.21

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

683

0

683

245043

37

1814

358.8

40.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.524 ± 0.083

0.613 ± 0.024

5.355 ± 0.07

7.282 ± 0.1

5.176 ± 0.079

5.374 ± 0.083

1.243 ± 0.035

9.964 ± 0.086

9.724 ± 0.096

9.02 ± 0.077

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.316 ± 0.046

7.252 ± 0.101

2.523 ± 0.044

3.045 ± 0.04

2.791 ± 0.057

6.52 ± 0.082

5.42 ± 0.079

6.062 ± 0.07

1.057 ± 0.031

3.739 ± 0.058
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski