Amino acid dipepetide frequency for Mesoplasma florum (strain ATCC 33453 / NBRC 100688 / NCTC 11704 / L1) (Acholeplasma florum)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.575AlaAla: 3.575 ± 0.183
0.351AlaCys: 0.351 ± 0.036
2.485AlaAsp: 2.485 ± 0.12
3.628AlaGlu: 3.628 ± 0.165
2.759AlaPhe: 2.759 ± 0.121
3.367AlaGly: 3.367 ± 0.134
0.792AlaHis: 0.792 ± 0.066
5.607AlaIle: 5.607 ± 0.186
5.554AlaLys: 5.554 ± 0.183
5.097AlaLeu: 5.097 ± 0.168
1.269AlaMet: 1.269 ± 0.078
3.35AlaAsn: 3.35 ± 0.12
1.388AlaPro: 1.388 ± 0.074
1.653AlaGln: 1.653 ± 0.091
1.824AlaArg: 1.824 ± 0.101
3.461AlaSer: 3.461 ± 0.126
3.265AlaThr: 3.265 ± 0.133
3.175AlaVal: 3.175 ± 0.153
0.584AlaTrp: 0.584 ± 0.05
2.053AlaTyr: 2.053 ± 0.1
0.0AlaXaa: 0.0 ± 0.0
Cys
0.282CysAla: 0.282 ± 0.036
0.082CysCys: 0.082 ± 0.021
0.392CysAsp: 0.392 ± 0.043
0.445CysGlu: 0.445 ± 0.042
0.38CysPhe: 0.38 ± 0.038
0.453CysGly: 0.453 ± 0.049
0.094CysHis: 0.094 ± 0.017
0.551CysIle: 0.551 ± 0.044
0.486CysLys: 0.486 ± 0.046
0.686CysLeu: 0.686 ± 0.054
0.155CysMet: 0.155 ± 0.022
0.326CysAsn: 0.326 ± 0.033
0.196CysPro: 0.196 ± 0.027
0.122CysGln: 0.122 ± 0.023
0.106CysArg: 0.106 ± 0.022
0.449CysSer: 0.449 ± 0.045
0.278CysThr: 0.278 ± 0.037
0.326CysVal: 0.326 ± 0.03
0.086CysTrp: 0.086 ± 0.02
0.241CysTyr: 0.241 ± 0.025
0.0CysXaa: 0.0 ± 0.0
Asp
2.84AspAla: 2.84 ± 0.11
0.351AspCys: 0.351 ± 0.037
2.714AspAsp: 2.714 ± 0.145
4.416AspGlu: 4.416 ± 0.163
3.493AspPhe: 3.493 ± 0.135
2.902AspGly: 2.902 ± 0.129
0.71AspHis: 0.71 ± 0.058
4.966AspIle: 4.966 ± 0.161
5.036AspLys: 5.036 ± 0.146
5.546AspLeu: 5.546 ± 0.165
1.134AspMet: 1.134 ± 0.085
3.065AspAsn: 3.065 ± 0.141
1.436AspPro: 1.436 ± 0.086
1.787AspGln: 1.787 ± 0.083
1.322AspArg: 1.322 ± 0.089
3.216AspSer: 3.216 ± 0.121
2.212AspThr: 2.212 ± 0.116
3.342AspVal: 3.342 ± 0.121
0.653AspTrp: 0.653 ± 0.052
2.404AspTyr: 2.404 ± 0.109
0.0AspXaa: 0.0 ± 0.0
Glu
4.187GluAla: 4.187 ± 0.151
0.359GluCys: 0.359 ± 0.04
3.281GluAsp: 3.281 ± 0.157
6.146GluGlu: 6.146 ± 0.238
3.595GluPhe: 3.595 ± 0.139
2.959GluGly: 2.959 ± 0.114
0.857GluHis: 0.857 ± 0.066
8.129GluIle: 8.129 ± 0.241
7.574GluLys: 7.574 ± 0.23
7.036GluLeu: 7.036 ± 0.192
1.885GluMet: 1.885 ± 0.095
5.766GluAsn: 5.766 ± 0.175
1.351GluPro: 1.351 ± 0.064
2.481GluGln: 2.481 ± 0.118
2.016GluArg: 2.016 ± 0.118
3.624GluSer: 3.624 ± 0.134
3.51GluThr: 3.51 ± 0.141
4.424GluVal: 4.424 ± 0.149
0.849GluTrp: 0.849 ± 0.054
2.787GluTyr: 2.787 ± 0.117
0.0GluXaa: 0.0 ± 0.0
Phe
2.934PheAla: 2.934 ± 0.113
0.294PheCys: 0.294 ± 0.033
3.432PheAsp: 3.432 ± 0.134
3.869PheGlu: 3.869 ± 0.148
2.493PhePhe: 2.493 ± 0.123
2.999PheGly: 2.999 ± 0.134
0.486PheHis: 0.486 ± 0.047
5.26PheIle: 5.26 ± 0.209
4.926PheLys: 4.926 ± 0.16
4.713PheLeu: 4.713 ± 0.175
1.147PheMet: 1.147 ± 0.069
3.575PheAsn: 3.575 ± 0.133
0.971PhePro: 0.971 ± 0.067
1.086PheGln: 1.086 ± 0.068
1.257PheArg: 1.257 ± 0.074
3.71PheSer: 3.71 ± 0.142
2.771PheThr: 2.771 ± 0.116
3.306PheVal: 3.306 ± 0.139
0.567PheTrp: 0.567 ± 0.063
1.963PheTyr: 1.963 ± 0.098
0.0PheXaa: 0.0 ± 0.0
Gly
3.069GlyAla: 3.069 ± 0.154
0.416GlyCys: 0.416 ± 0.051
2.547GlyAsp: 2.547 ± 0.123
3.171GlyGlu: 3.171 ± 0.123
2.861GlyPhe: 2.861 ± 0.129
3.199GlyGly: 3.199 ± 0.158
0.869GlyHis: 0.869 ± 0.074
5.562GlyIle: 5.562 ± 0.183
4.514GlyLys: 4.514 ± 0.16
5.183GlyLeu: 5.183 ± 0.167
1.506GlyMet: 1.506 ± 0.088
2.8GlyAsn: 2.8 ± 0.108
1.11GlyPro: 1.11 ± 0.074
1.694GlyGln: 1.694 ± 0.085
1.734GlyArg: 1.734 ± 0.113
3.314GlySer: 3.314 ± 0.164
3.424GlyThr: 3.424 ± 0.132
3.873GlyVal: 3.873 ± 0.161
0.669GlyTrp: 0.669 ± 0.057
2.228GlyTyr: 2.228 ± 0.106
0.0GlyXaa: 0.0 ± 0.0
His
0.833HisAla: 0.833 ± 0.057
0.094HisCys: 0.094 ± 0.02
0.726HisAsp: 0.726 ± 0.055
0.93HisGlu: 0.93 ± 0.058
0.747HisPhe: 0.747 ± 0.056
0.808HisGly: 0.808 ± 0.058
0.224HisHis: 0.224 ± 0.031
1.106HisIle: 1.106 ± 0.061
1.057HisLys: 1.057 ± 0.073
0.979HisLeu: 0.979 ± 0.062
0.241HisMet: 0.241 ± 0.029
0.796HisAsn: 0.796 ± 0.057
0.412HisPro: 0.412 ± 0.043
0.404HisGln: 0.404 ± 0.05
0.408HisArg: 0.408 ± 0.041
0.804HisSer: 0.804 ± 0.053
0.535HisThr: 0.535 ± 0.049
0.694HisVal: 0.694 ± 0.056
0.147HisTrp: 0.147 ± 0.027
0.49HisTyr: 0.49 ± 0.042
0.0HisXaa: 0.0 ± 0.0
Ile
5.738IleAla: 5.738 ± 0.189
0.898IleCys: 0.898 ± 0.076
5.909IleAsp: 5.909 ± 0.155
7.28IleGlu: 7.28 ± 0.208
4.95IlePhe: 4.95 ± 0.179
5.256IleGly: 5.256 ± 0.187
0.959IleHis: 0.959 ± 0.073
9.48IleIle: 9.48 ± 0.269
9.725IleLys: 9.725 ± 0.203
9.072IleLeu: 9.072 ± 0.242
2.061IleMet: 2.061 ± 0.104
7.582IleAsn: 7.582 ± 0.188
2.677IlePro: 2.677 ± 0.109
2.771IleGln: 2.771 ± 0.106
2.608IleArg: 2.608 ± 0.115
7.542IleSer: 7.542 ± 0.204
5.048IleThr: 5.048 ± 0.171
5.901IleVal: 5.901 ± 0.172
0.747IleTrp: 0.747 ± 0.05
3.436IleTyr: 3.436 ± 0.12
0.0IleXaa: 0.0 ± 0.0
Lys
4.86LysAla: 4.86 ± 0.143
0.441LysCys: 0.441 ± 0.052
5.456LysAsp: 5.456 ± 0.169
8.558LysGlu: 8.558 ± 0.178
4.105LysPhe: 4.105 ± 0.143
4.191LysGly: 4.191 ± 0.15
1.273LysHis: 1.273 ± 0.075
9.48LysIle: 9.48 ± 0.229
9.309LysLys: 9.309 ± 0.32
7.586LysLeu: 7.586 ± 0.222
2.836LysMet: 2.836 ± 0.097
8.072LysAsn: 8.072 ± 0.207
2.628LysPro: 2.628 ± 0.119
3.714LysGln: 3.714 ± 0.114
2.722LysArg: 2.722 ± 0.111
5.244LysSer: 5.244 ± 0.146
5.852LysThr: 5.852 ± 0.143
5.787LysVal: 5.787 ± 0.179
1.045LysTrp: 1.045 ± 0.075
4.077LysTyr: 4.077 ± 0.136
0.0LysXaa: 0.0 ± 0.0
Leu
4.889LeuAla: 4.889 ± 0.165
0.563LeuCys: 0.563 ± 0.045
4.779LeuAsp: 4.779 ± 0.152
5.864LeuGlu: 5.864 ± 0.208
4.877LeuPhe: 4.877 ± 0.195
4.709LeuGly: 4.709 ± 0.17
0.975LeuHis: 0.975 ± 0.068
9.092LeuIle: 9.092 ± 0.249
9.007LeuLys: 9.007 ± 0.201
7.611LeuLeu: 7.611 ± 0.184
2.04LeuMet: 2.04 ± 0.101
7.407LeuAsn: 7.407 ± 0.202
2.461LeuPro: 2.461 ± 0.09
2.514LeuGln: 2.514 ± 0.097
2.6LeuArg: 2.6 ± 0.109
6.395LeuSer: 6.395 ± 0.16
5.428LeuThr: 5.428 ± 0.153
5.644LeuVal: 5.644 ± 0.154
0.82LeuTrp: 0.82 ± 0.056
2.53LeuTyr: 2.53 ± 0.114
0.0LeuXaa: 0.0 ± 0.0
Met
1.514MetAla: 1.514 ± 0.098
0.155MetCys: 0.155 ± 0.028
1.0MetAsp: 1.0 ± 0.071
1.2MetGlu: 1.2 ± 0.068
1.298MetPhe: 1.298 ± 0.098
1.363MetGly: 1.363 ± 0.088
0.322MetHis: 0.322 ± 0.042
2.347MetIle: 2.347 ± 0.116
2.302MetLys: 2.302 ± 0.106
2.024MetLeu: 2.024 ± 0.099
0.526MetMet: 0.526 ± 0.054
1.69MetAsn: 1.69 ± 0.081
0.869MetPro: 0.869 ± 0.066
0.747MetGln: 0.747 ± 0.057
0.669MetArg: 0.669 ± 0.052
1.8MetSer: 1.8 ± 0.105
1.143MetThr: 1.143 ± 0.072
1.257MetVal: 1.257 ± 0.079
0.245MetTrp: 0.245 ± 0.029
0.69MetTyr: 0.69 ± 0.047
0.0MetXaa: 0.0 ± 0.0
Asn
3.293AsnAla: 3.293 ± 0.141
0.322AsnCys: 0.322 ± 0.036
4.126AsnAsp: 4.126 ± 0.134
5.934AsnGlu: 5.934 ± 0.186
3.918AsnPhe: 3.918 ± 0.159
3.616AsnGly: 3.616 ± 0.123
0.837AsnHis: 0.837 ± 0.061
6.84AsnIle: 6.84 ± 0.193
8.027AsnLys: 8.027 ± 0.194
6.734AsnLeu: 6.734 ± 0.215
1.334AsnMet: 1.334 ± 0.083
5.754AsnAsn: 5.754 ± 0.222
1.996AsnPro: 1.996 ± 0.098
2.6AsnGln: 2.6 ± 0.125
1.673AsnArg: 1.673 ± 0.087
4.705AsnSer: 4.705 ± 0.166
3.036AsnThr: 3.036 ± 0.135
3.852AsnVal: 3.852 ± 0.133
0.935AsnTrp: 0.935 ± 0.079
2.991AsnTyr: 2.991 ± 0.125
0.0AsnXaa: 0.0 ± 0.0
Pro
1.343ProAla: 1.343 ± 0.089
0.11ProCys: 0.11 ± 0.02
1.053ProAsp: 1.053 ± 0.067
1.943ProGlu: 1.943 ± 0.1
1.465ProPhe: 1.465 ± 0.08
1.502ProGly: 1.502 ± 0.089
0.412ProHis: 0.412 ± 0.042
2.685ProIle: 2.685 ± 0.112
2.114ProLys: 2.114 ± 0.099
2.293ProLeu: 2.293 ± 0.099
0.551ProMet: 0.551 ± 0.061
1.763ProAsn: 1.763 ± 0.076
0.416ProPro: 0.416 ± 0.037
0.604ProGln: 0.604 ± 0.047
0.604ProArg: 0.604 ± 0.055
1.738ProSer: 1.738 ± 0.078
1.579ProThr: 1.579 ± 0.079
1.771ProVal: 1.771 ± 0.094
0.286ProTrp: 0.286 ± 0.035
0.996ProTyr: 0.996 ± 0.07
0.0ProXaa: 0.0 ± 0.0
Gln
1.841GlnAla: 1.841 ± 0.085
0.065GlnCys: 0.065 ± 0.015
1.359GlnAsp: 1.359 ± 0.086
2.216GlnGlu: 2.216 ± 0.105
1.359GlnPhe: 1.359 ± 0.068
1.583GlnGly: 1.583 ± 0.075
0.335GlnHis: 0.335 ± 0.033
3.428GlnIle: 3.428 ± 0.124
3.089GlnLys: 3.089 ± 0.115
2.567GlnLeu: 2.567 ± 0.096
0.694GlnMet: 0.694 ± 0.051
2.42GlnAsn: 2.42 ± 0.112
0.751GlnPro: 0.751 ± 0.058
1.183GlnGln: 1.183 ± 0.082
0.939GlnArg: 0.939 ± 0.065
1.665GlnSer: 1.665 ± 0.092
1.738GlnThr: 1.738 ± 0.085
1.82GlnVal: 1.82 ± 0.086
0.306GlnTrp: 0.306 ± 0.037
1.094GlnTyr: 1.094 ± 0.064
0.0GlnXaa: 0.0 ± 0.0
Arg
1.473ArgAla: 1.473 ± 0.095
0.175ArgCys: 0.175 ± 0.029
1.555ArgAsp: 1.555 ± 0.102
1.938ArgGlu: 1.938 ± 0.101
1.343ArgPhe: 1.343 ± 0.073
1.457ArgGly: 1.457 ± 0.099
0.347ArgHis: 0.347 ± 0.034
2.795ArgIle: 2.795 ± 0.1
2.816ArgLys: 2.816 ± 0.127
2.334ArgLeu: 2.334 ± 0.104
0.841ArgMet: 0.841 ± 0.064
1.914ArgAsn: 1.914 ± 0.114
0.808ArgPro: 0.808 ± 0.061
0.849ArgGln: 0.849 ± 0.072
1.106ArgArg: 1.106 ± 0.099
1.445ArgSer: 1.445 ± 0.092
1.645ArgThr: 1.645 ± 0.103
1.632ArgVal: 1.632 ± 0.091
0.249ArgTrp: 0.249 ± 0.029
1.188ArgTyr: 1.188 ± 0.085
0.0ArgXaa: 0.0 ± 0.0
Ser
3.448SerAla: 3.448 ± 0.131
0.363SerCys: 0.363 ± 0.038
3.493SerAsp: 3.493 ± 0.14
4.212SerGlu: 4.212 ± 0.139
3.624SerPhe: 3.624 ± 0.148
3.759SerGly: 3.759 ± 0.15
0.869SerHis: 0.869 ± 0.062
6.607SerIle: 6.607 ± 0.162
6.632SerLys: 6.632 ± 0.184
5.758SerLeu: 5.758 ± 0.194
1.306SerMet: 1.306 ± 0.076
4.75SerAsn: 4.75 ± 0.169
1.359SerPro: 1.359 ± 0.076
1.755SerGln: 1.755 ± 0.084
1.779SerArg: 1.779 ± 0.089
4.64SerSer: 4.64 ± 0.176
3.824SerThr: 3.824 ± 0.145
3.901SerVal: 3.901 ± 0.116
0.649SerTrp: 0.649 ± 0.055
2.469SerTyr: 2.469 ± 0.104
0.0SerXaa: 0.0 ± 0.0
Thr
2.71ThrAla: 2.71 ± 0.116
0.261ThrCys: 0.261 ± 0.031
2.848ThrAsp: 2.848 ± 0.178
3.477ThrGlu: 3.477 ± 0.137
3.085ThrPhe: 3.085 ± 0.118
3.563ThrGly: 3.563 ± 0.133
0.702ThrHis: 0.702 ± 0.055
5.444ThrIle: 5.444 ± 0.162
4.836ThrLys: 4.836 ± 0.13
5.007ThrLeu: 5.007 ± 0.13
1.134ThrMet: 1.134 ± 0.076
3.914ThrAsn: 3.914 ± 0.143
1.812ThrPro: 1.812 ± 0.093
1.51ThrGln: 1.51 ± 0.084
1.441ThrArg: 1.441 ± 0.082
3.852ThrSer: 3.852 ± 0.135
3.212ThrThr: 3.212 ± 0.131
3.008ThrVal: 3.008 ± 0.175
0.522ThrTrp: 0.522 ± 0.067
1.865ThrTyr: 1.865 ± 0.095
0.0ThrXaa: 0.0 ± 0.0
Val
3.836ValAla: 3.836 ± 0.145
0.49ValCys: 0.49 ± 0.051
3.477ValAsp: 3.477 ± 0.141
4.322ValGlu: 4.322 ± 0.16
2.808ValPhe: 2.808 ± 0.123
3.452ValGly: 3.452 ± 0.146
0.69ValHis: 0.69 ± 0.055
5.852ValIle: 5.852 ± 0.191
5.868ValLys: 5.868 ± 0.162
5.432ValLeu: 5.432 ± 0.168
1.416ValMet: 1.416 ± 0.077
3.975ValAsn: 3.975 ± 0.137
1.465ValPro: 1.465 ± 0.086
1.473ValGln: 1.473 ± 0.081
1.632ValArg: 1.632 ± 0.088
4.248ValSer: 4.248 ± 0.142
3.367ValThr: 3.367 ± 0.181
4.167ValVal: 4.167 ± 0.167
0.543ValTrp: 0.543 ± 0.05
2.106ValTyr: 2.106 ± 0.101
0.0ValXaa: 0.0 ± 0.0
Trp
0.498TrpAla: 0.498 ± 0.052
0.033TrpCys: 0.033 ± 0.011
0.579TrpAsp: 0.579 ± 0.064
0.669TrpGlu: 0.669 ± 0.06
0.612TrpPhe: 0.612 ± 0.055
0.494TrpGly: 0.494 ± 0.046
0.11TrpHis: 0.11 ± 0.02
1.183TrpIle: 1.183 ± 0.083
0.82TrpLys: 0.82 ± 0.054
0.951TrpLeu: 0.951 ± 0.063
0.363TrpMet: 0.363 ± 0.04
0.959TrpAsn: 0.959 ± 0.085
0.192TrpPro: 0.192 ± 0.026
0.249TrpGln: 0.249 ± 0.033
0.302TrpArg: 0.302 ± 0.036
0.702TrpSer: 0.702 ± 0.064
0.653TrpThr: 0.653 ± 0.052
0.616TrpVal: 0.616 ± 0.055
0.208TrpTrp: 0.208 ± 0.029
0.375TrpTyr: 0.375 ± 0.039
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.073TyrAla: 2.073 ± 0.093
0.31TyrCys: 0.31 ± 0.033
2.334TyrAsp: 2.334 ± 0.101
2.6TyrGlu: 2.6 ± 0.108
1.987TyrPhe: 1.987 ± 0.097
2.11TyrGly: 2.11 ± 0.103
0.571TyrHis: 0.571 ± 0.049
3.224TyrIle: 3.224 ± 0.114
3.546TyrLys: 3.546 ± 0.127
3.595TyrLeu: 3.595 ± 0.131
0.714TyrMet: 0.714 ± 0.053
2.644TyrAsn: 2.644 ± 0.121
0.93TyrPro: 0.93 ± 0.059
1.257TyrGln: 1.257 ± 0.071
1.167TyrArg: 1.167 ± 0.071
2.649TyrSer: 2.649 ± 0.124
1.685TyrThr: 1.685 ± 0.094
2.122TyrVal: 2.122 ± 0.093
0.461TyrTrp: 0.461 ± 0.041
1.412TyrTyr: 1.412 ± 0.083
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 683 proteins (245043 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski