Pseudaminobacter arsenicus
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4868 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A432V0V4|A0A432V0V4_9RHIZ Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase GatCAB subunit C OS=Pseudaminobacter arsenicus OX=1851146 GN=EET67_21200 PE=3 SV=1
MM1 pKa = 7.43 SKK3 pKa = 10.34 LARR6 pKa = 11.84 ILAIVFLAVFAASTAAQVGTATDD29 pKa = 3.65 MSLTTMDD36 pKa = 4.99 SGMADD41 pKa = 3.53 CQGCPGDD48 pKa = 4.39 DD49 pKa = 3.73 AQASTCDD56 pKa = 3.63 QFCVTVLAAICLPTATEE73 pKa = 4.05 LPYY76 pKa = 10.96 VAVIDD81 pKa = 3.9 SAPPGEE87 pKa = 4.29 QSDD90 pKa = 4.05 GRR92 pKa = 11.84 TGPPDD97 pKa = 4.29 PYY99 pKa = 10.02 PPRR102 pKa = 11.84 SIVV105 pKa = 2.97
Molecular weight: 10.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.783
IPC2_protein 3.795
IPC_protein 3.732
Toseland 3.516
ProMoST 3.948
Dawson 3.757
Bjellqvist 3.923
Wikipedia 3.757
Rodwell 3.567
Grimsley 3.427
Solomon 3.732
Lehninger 3.681
Nozaki 3.897
DTASelect 4.164
Thurlkill 3.605
EMBOSS 3.757
Sillero 3.859
Patrickios 0.401
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.793
Protein with the highest isoelectric point:
>tr|A0A432V9G6|A0A432V9G6_9RHIZ HTH luxR-type domain-containing protein OS=Pseudaminobacter arsenicus OX=1851146 GN=EET67_05835 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.27 QPSKK9 pKa = 9.73 LVRR12 pKa = 11.84 KK13 pKa = 9.15 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.25 GGRR28 pKa = 11.84 GVIAARR34 pKa = 11.84 RR35 pKa = 11.84 SRR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.96 RR41 pKa = 11.84 LSAA44 pKa = 4.03
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4868
0
4868
1502345
27
2854
308.6
33.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.209 ± 0.039
0.86 ± 0.012
5.551 ± 0.03
5.945 ± 0.032
3.87 ± 0.023
8.507 ± 0.031
2.061 ± 0.015
5.546 ± 0.025
3.565 ± 0.026
9.921 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.591 ± 0.016
2.676 ± 0.016
4.973 ± 0.024
3.056 ± 0.018
6.977 ± 0.035
5.566 ± 0.022
5.192 ± 0.021
7.366 ± 0.029
1.295 ± 0.015
2.273 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here